Ask about this productRelated genes to: TEKT1 antibody
- Gene:
- TEKT1 NIH gene
- Name:
- tektin 1
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 17p13.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-04-25
- Date modifiied:
- 2016-10-05
Related products to: TEKT1 antibody
Related articles to: TEKT1 antibody
- Rotenone is a widely used environmental pesticide, and epidemiological studies suggest that exposure is associated with an increased risk of Parkinson's disease (PD); however, the molecular toxicological basis of this association remains incompletely defined. Ferroptosis is an iron-dependent, lipid peroxidation-driven form of regulated cell death that is relevant to PD and other neurodegenerative disorders. In this study, we provide disease-contextual functional evidence linking ferroptosis to rotenone-induced PD-like neurotoxicity. We combined network toxicology, human PD substantia nigra transcriptomic analysis using GSE7621, and SH-SY5Y cell-based validation. Rotenone-associated targets were predicted and analyzed for ferroptosis-related enrichment, PD transcriptomic signatures were used for disease-contextual candidate prioritization, and selected findings were validated using qPCR, CCK-8, Western blotting, C11-BODIPY lipid peroxidation staining, and transmission electron microscopy. : By further integrating a human PD substantia nigra transcriptomic dataset (GSE7621), we prioritized an 11-gene, PD-contextualized ferroptosis-associated candidate module (, , , , , , , , , , and ). In SH-SY5Y cells, rotenone exposure consistently upregulated this candidate transcriptional module, and this induction was broadly attenuated by the ferroptosis inhibitor ferrostatin-1 (Fer-1). In parallel, orthogonal functional assays supported an iron- and lipid peroxidation-driven injury state under rotenone exposure that was suppressible by ferroptosis inhibition and iron chelation. Finally, we further performed an exploratory drug-gene association screen to prioritize clinically available candidates, and a limited qPCR check suggested that several selected compounds partially attenuated representative hub-gene induction under rotenone exposure. : Collectively, these findings provide disease-contextual and experimentally supported evidence linking rotenone exposure to ferroptosis-associated neurotoxicity, and identify a ferroptosis-responsive transcriptional module for future hypothesis-driven mechanistic investigation. - Source: PubMed
Publication date: 2026/05/22
Chen YimengZhang DingMa JiajiaLi HuixinXu JingrongMa CuixiaLiu YuqianZhao ZhenbingDuffy Garry PMa JunCui Huixian - Deoxynivalenol (DON) is a common mycotoxin linked to ovarian oxidative stress, toxicity, and reduced reproductive performance. Fermented Chinese chive is known for its antioxidant properties and potential reproductive benefits, but their individual and combined effects on ovarian function remain unclear in post-pubertal mice. In this study, a 21-day oral gavage model in female Kunming mice was used to evaluate the effects of DON (2 mg/kg/day), fermented Chinese chive extract (LEEK; 0.2 mL/day), and their combined exposure (LKDON) on ovarian physiology, oocyte quality, and ovarian transcriptomic responses. The results showed that DON exposure significantly reduced the zygote cleavage rate, increased intracellular reactive oxygen species levels, and disrupted oocyte mitochondrial membrane potential. While histological examination revealed disturbed follicular architecture. Transcriptomic hub gene analysis showed that DON exposure down-regulate the key associated with innate immune responses and motile cilia/axonemal structure, including , , , , and . In contrast, LEEK alone was associated with immunomodulatory upregulated genes, including , , and . Interestingly, LKDON and DON comparison revealed upregulation of a motile cilia/axoneme gene network (, , , , , and ), rather than a global reversal of DON-induced changes. Overall, finding suggest that DON disrupts ovarian immune and structural pathways, while fermented Chinese chive provides partial protection by modulating specific biological processes. Further studies are needed to confirm the underlying mechanisms. - Source: PubMed
Publication date: 2026/04/01
Zou HongQin Chun-YanSuthar Teerath KumarXie YupengAbednicco Koroloso PhomaneWang Chun-FengKim Min KyuZhang Shu-MinSun Wu-Sheng - Doublet microtubule (DMT)-associated proteins assemble and drive sperm flagella, which are essential for successful fertilization. However, the exact roles of different DMT-associated proteins in sperm function and the underlying molecular mechanisms remain elusive. Here, we generate four gene-knockout mice based on high-resolution structures targeting distinct DMT components: two intermediate filament-like tektins (TEKT1, TEKT5) and two enzymes (TSSK6, DUSP21). The depletion of TEKT1, shared by sperm flagella and motile cilia, causes male infertility characterized by impaired sperm motility and loss of the tektin bundle, whereas sperm-specific Tekt5 knockout (KO) mice remain fertile with largely normal flagellar function, indicating functional divergence within the tektin family. Tssk6 KO spermatozoa exhibit severely disturbed morphology and motility, resulting in homozygote infertility and heterozygote subfertility. Phosphoproteomics reveals dysregulated phosphorylation of axonemal proteins, highlighting the critical role of kinase-mediated signaling in regulating sperm motility. Conversely, Dusp21 KO mice display no fertility or sperm motility defects, suggesting compensatory phosphatase activity. Phenotypic comparisons between Tekt1 and Tssk6 KO mice suggest their involvement in distinct subtypes of asthenozoospermia. Overall, this study elucidates how filamentous and enzymatic DMT proteins govern sperm function through divergent mechanisms, which have implications for molecular diagnosis of male infertility. - Source: PubMed
Publication date: 2026/02/28
Liu QiZhou LunniLiang XiaochenChen PengyuLi BoYang ShuoLiu YuqiZhao HaiboHu JinFeng ShanXie ShanshanWu JianpingGui Miao - Non-small cell lung cancer (NSCLC) is responsible for inducing approximately 85% of lung cancers and remains a major cause of worldwide cancer deaths. This study employed an exhaustive bioinformatics pipeline for comprehensive analysis of high-throughput RNA-sequencing data from The Cancer Genome Atlas (TCGA) to discover important molecular signatures and probable therapeutic targets in NSCLC. A total of 8,536 differential expression genes (DEGs) were identified, of which 6,241 were upregulated and 2,295 were downregulated gene expressions. Protein-protein interaction network analysis and CytoHubba-based prioritization for discovery of hub genes identified twenty core genes, out of which, CYP2B6, TEKT1, UGT1A1, UGT1A7, UGT1A8 RPTN, and AOX1 presented exceptional network centrality. Functional enrichment implicated drug metabolism, xenobiotic degradation, and chemical carcinogenesis. Mutational profiling identified frequent mutations in AOX1, RPTN, and CYP2B6. Kaplan-Meier survival analysis linked multiple expressions of hub genes to unfavorable survival. miRNA-mRNA interaction analysis uncovered conserved regulatory partnerships between miR-203 and miR-141/200a families and implicated post-transcriptional control in NSCLC pathogenesis. The drug-gene interaction analysis nominated CYP2B6 to be a key druggable target exhibitting interaction to multiple FDA drugs. The molecular docking and 500 ns molecular dynamics simulation between CYP2B6 and Pazopanib, Nilotinib, and Sorafenib validated directional and high-affinity interaction and verified RMSD, RMSF, and contact profiling. This all-encompassing study shortlists NSCLC biomarker candidates and druggable targets and gives mechanism-based understanding to disease development and drug resistance. This study gives impetus for precision medicine therapies development and suggests CYP2B6 to be a potential drug prospect. - Source: PubMed
Publication date: 2025/12/17
Tripathi VarshaKhare AishwaryaDwivedi Vivek DharRanjan Vandana - Bone Morphogenetic Proteins have been reported to play important roles in developmental biology across the species. The present study investigates the role of BMP4 in inducing germ lineage development. For this purpose, buffalo ES cells were subjected to in vitro differentiation under suspension as well as adherent cultures, at different BMP4 doses and time periods. ES cell colonies were differentiated as EBs as well as adherent monolayers under different BMP4 concentrations (20, 50 and 100 ngml) for different time intervals (4, 8 and 14 days). The differentiated cultures were subjected to transcriptional profiling for germ lineage associated genes. qPCR data analysis revealed that BMP4 at a concentration of 50-100 ngml and for a period of 14 days led to maximum induction of such germ lineage associated genes like DAZL, VASA, PLZF (PGC-specific); SYCP3, MLH1, TNP1/2 and PRM2 (Meiotic genes); BOULE and TEKT1 (Spermatocyte markers); and GDF9, ZP2 and 3 (Oocyte markers). Immunocytochemical analysis of the differentiated cultures revealed positive expression of PGC-markers (c-KIT, DAZL and VASA), Meiotic-markers (SYCP3, MLH1 and PROTAMINE1), Spermatocyte-markers (ACROSIN and HAPRIN) and Oocyte-markers (GDF9 and ZP4), marking thereby the differentiation towards germ lineage cells. Oocyte-like structures (OLS) obtained in monolayer differentiated cultures harbored a big nucleus covered with a ZP4 coat, so typical of an oocyte. These OLS, in extended cultures, showed embryonic development and progressed through different embryonic-like structures. Global DNA methylation analysis of the optimally differentiated cultures showed significantly (p < 0.05) decreased levels of 5-methyl-2-deoxycytidine, a mimic of the methylation erasure process typical of gametogenesis. Expression of PGC markers, methylation erasure, and meiotic markers together with expression of spermatocyte and oocyte markers is suggestive of post-meiotic progression into spermatogenesis and oogenesis, respectively- thus reflecting onset of in vitro gametogenesis. - Source: PubMed
Publication date: 2025/09/26
Shah Syed MohmadChauhan Manmohan Singh