Ask about this productRelated genes to: SPIRE1 antibody
- Gene:
- SPIRE1 NIH gene
- Name:
- spire type actin nucleation factor 1
- Previous symbol:
- -
- Synonyms:
- spir-1, KIAA1135
- Chromosome:
- 18p11.21
- Locus Type:
- gene with protein product
- Date approved:
- 2004-04-28
- Date modifiied:
- 2018-11-19
Related products to: SPIRE1 antibody
Related articles to: SPIRE1 antibody
- Cellular uptake and intracellular distribution of phosphorothioate-modified antisense oligonucleotides (PS-ASOs) are mediated by protein interactions. While several PS-ASOs-binding proteins have been identified, mainly using gapmer designs with 2'-O-methoxyethyl (2'MOE) modifications, less is known about protein partners of splice-switching oligonucleotides (SSOs) with alternative ribose modifications. Here, using affinity purification mass spectrometry (AP-MS), we identified the intracellular protein partners of PS-SSOs of the same sequence with three distinct ribose modifications: tricyclo-DNA (tcDNA), locked nucleic acid (LNA), and 2'MOE. Interestingly, we found previously reported PS interactors, such as GRSF1, NONO, and NCL, as well as uncharacterized protein partners. Four shared interactors identified in this study, ERC1, SPIRE1, THRAP3, and GOLGA2, were selected based on functional relevance and tested for their impact on exon skipping efficacy using PS-SSOs targeting the human Duchenne muscular dystrophy (DMD) transcript. RNA interference-mediated knockdown of each protein led to a reduction of exon skipping efficiency, suggesting that these proteins may contribute to PS-ASOs activity regardless of their sugar modifications. Overall, our results provide a set of intracellular protein interactors of different PS-ASOs, representing a valuable resource to explore mechanisms underlying their activity and offering potential leads for the optimization of oligonucleotide therapeutics. - Source: PubMed
Publication date: 2026/01/12
Gaci ArisMenchon GrégoryBruce JohannaSalnot VirginiePedeux RémyGoyenvalle Aurélie - Moyamoya disease (MMD) is a rare chronic cerebrovascular disorder, yet the role of mitochondria in its pathogenesis remains unclear, hindering progress in understanding pathological mechanisms and identifying therapeutic targets. However, mitochondria-related genes (MiRGs) have not been systematically investigated in MMD, and their pathogenic relevance remains unclear. This study aimed to identify MMD-associated MiRGs, evaluate their diagnostic potential through integrative bioinformatics and machine learning, and validate key findings experimentally. We retrieved two MMD transcriptomic datasets (GSE157628, GSE141025) from the Gene Expression Omnibus (GEO) and obtained MiRGs from MitoCarta 3.0. Three machine learning methods were used to screen hub genes, with validation via an independent cohort (GSE141025) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Three hub MiRGs (SLC25A20, METAP1D, and SPIRE1) were confirmed to be associated with MMD, as supported by transcriptomic analysis, machine learning, and RT-qPCR validation. As the first comprehensive study to systematically explore MiRGs in MMD-distinct from prior work focused on mitochondrial function or clinical observations-our findings provide potential diagnostic biomarkers for early MMD detection and guidance for developing therapies targeting mitochondrial function restoration. - Source: PubMed
Publication date: 2025/11/14
Yu JianshuLi XiuyunLiu Wenqiang - PCSK9 inhibitors are a novel class of medications that lower LDL cholesterol (LDL-C) by increasing LDL receptor activity, promoting clearance of LDL-C from the bloodstream. Over the years, PCSK9 inhibitors have been explored as adjunct therapies to statins or as monotherapy in high-risk cardiovascular patients. - Source: PubMed
Publication date: 2024/10/30
Jeswani Bijay MukeshSharma ShubhangiRathore Sawai SinghNazir AbubakarBhatheja RohitKapoor Kapil - The genetic basis underlying spawning abilities in the Pacific white shrimp, Penaeus vannamei, remains largely unexplored. To investigate genetic variations potentially related to reproductive performance, a systematic bioinformatic analysis was conducted to identify structural variations (SVs) with different polymorphic spectra in P. vannamei with high fertility (HF) and low fertility (LF). - Source: PubMed
Publication date: 2024/10/07
Huang YongyuWang HaoXu ShengyuLiu JinliZeng QifanHu JingjieBao Zhenmin - An important question in cell biology is how cytoskeletal proteins evolved and drove the development of novel structures and functions. Here we address the origin of SPIRE actin nucleators. Mammalian SPIREs work with RAB GTPases, formin (FMN)-subgroup actin assembly proteins and class-5 myosin (MYO5) motors to transport organelles along actin filaments towards the cell membrane. However, the origin and extent of functional conservation of SPIRE among species is unknown. Our sequence searches show that SPIRE exist throughout holozoans (animals and their closest single-celled relatives), but not other eukaryotes. SPIRE from unicellular holozoans (choanoflagellate), interacts with RAB, FMN and MYO5 proteins, nucleates actin filaments and complements mammalian SPIRE function in organelle transport. Meanwhile SPIRE and MYO5 proteins colocalise to organelles in Salpingoeca rosetta choanoflagellates. Based on these observations we propose that SPIRE originated in unicellular ancestors of animals providing an actin-myosin driven exocytic transport mechanism that may have contributed to the evolution of complex multicellular animals. - Source: PubMed
Publication date: 2024/07/08
Kollmar MartinWelz TobiasRavi AishwaryaKaufmann ThomasAlzahofi NouraHatje KlasAlghamdi AsmahanKim JiyuBriggs Deborah ASamol-Wolf AnnettePylypenko OlenaHume Alistair NBurkhardt PawelFaix JanKerkhoff Eugen