Ask about this productRelated genes to: SPERT antibody
- Gene:
- SPERT NIH gene
- Name:
- spermatid associated
- Previous symbol:
- -
- Synonyms:
- NURIT, CBY2
- Chromosome:
- 13q14.13
- Locus Type:
- gene with protein product
- Date approved:
- 2006-03-14
- Date modifiied:
- 2016-06-07
Related products to: SPERT antibody
Related articles to: SPERT antibody
- Feathers are valuable by-products of the poultry industry, and their growth and development markedly have significant impact on economic returns. This study used Landes geese as a model to analyze the expression of core genes involved in feather regeneration and inflammation through comparative transcriptomic analyzes between plucked and unplucked skin tissues. In Landes geese aged 180 days (LD180), 624 differentially expressed genes (DEGs) were identified at 5 days post-plucking (481 upregulated and 143 downregulated), whereas 1851 DEGs were identified at 14 days (1350 upregulated and 501 downregulated). In Landes geese aged 90 days (LD90), 694 DEGs were detected at five days post-plucking (513 upregulated and 181 downregulated), with 1154 DEGs identified at 14 days (887 upregulated and 267 downregulated). These DEGs were significantly enriched in GO terms, KEGG pathways, and protein-protein interaction networks associated with inflammatory responses (e.g., leukocyte-mediated immunity), and feather regeneration (e.g., epidermis development). Notably, signatures of both innate and adaptive immunity were detected at the early post-plucking stage. Compared with younger geese, the LD180 group showed faster inflammatory resolution than the LD90 group, as demonstrated by a more pronounced decrease in proinflammatory gene expression levels in LD180. Comparative analysis revealed that mechanical damage (such as feather plucking) induces immediate inflammatory and anti-inflammatory responses. In conclusion, the mechanical damage caused by feather plucking triggers a renewed cycle of feather growth, which requires overcoming the harm inflicted by the subsequent inflammatory responses. The ability to recover from inflammatory damage varies significantly with age. - Source: PubMed
Liu HaodongYang YunzhouZhai ShaojiaWang HuiyingHe Daqian - BACKGROUND: LCORL is a crucial gene that regulates body weight and size in several organisms, including the graylag geese (Anser anser). However, the effect of this gene in swan geese (Anser cygnoides) has been deemed negligible. As various indigenous goose breeds (A. cygnoides) are systemically selected for their growth performance, a comprehensive understanding of the genetic basis of growth performance-related traits is imperative for goose breeding. RESULTS: A goose double-digest genotyping-by-sequencing (ddGBS) protocol was established by implementing dual restriction enzyme (EcoRI and BfaI) digestion of genomic DNA, followed by the ligation of adapters with paired-end index sequences. The ddGBS method was used to perform genome-wide association studies on body weight in Zhedong white goose, with the aim to identify candidate genes. The novel genome-wide genotyping method detected > 100 K bi-allelic markers per sample and exhibited a high genotyping accuracy demonstrated by the Sanger sequencing method. Moreover, this strategy was used to conduct a longitudinal genome-wide association study (GWAS) on body-weight traits (0–10 weeks), which identified 19 significant loci. These loci exerted significant effects on body weight, but could only explain a small proportion of the phenotypic variation (≤ 0.51%). Nine candidate genes, including growth-related genes (e.g., THADA), were identified in the proximity of significant GWAS markers. The GBS-based GWAS on body size detected candidate variants associated with shank circumference. Each of the four significant single-nucleotide polymorphisms explained 0.57%–0.58% of the phenotypic variance. Furthermore, screening analyses of genomic selection signals between high- and low-body-weight groups at 10 weeks of age detected 754 potential loci under selection. However, no extremely divergent regions were identified in the genome. CONCLUSIONS: The modified goose ddGBS approach provided an alternative genotyping workflow for geese, facilitating GWAS on quantitative traits. The preliminary findings of this study revealed that several genetic loci with minor effects, rather than single major genes, regulated body-weight and body-size traits in Zhedong white goose. Furthermore, short-term artificial selection pressure on body-weight traits could improve phenotypes. However, it mildly reshaped the genome on a large scale. - Source: PubMed
Publication date: 2025/12/12
Yang YunzhouZhai ShaojiaLiu HaodongWang HuiyingHe Daqian - Dysregulation of microRNAs (miRNAs) is closely linked to the progression of diverse human diseases. However, the lack of a standardized, fine-grained dataset of miRNA-disease regulatory interactions and the limited ability of existing methods to capture multi-level regulatory relations hinder full understanding of these mechanisms. - Source: PubMed
Publication date: 2025/11/27
Shi JiangchengWang LijunLiu LixueSu Jinhao - Intracytoplasmic spermatid injection into oocytes has limited efficiency in cattle, with no offspring generated so far, partly due to ambiguous spermatid identification. This study aimed to develop and validate a method for isolating and characterizing bovine spermatids to improve the efficiency of spermatid intracytoplasmic injection. First, we optimized a protocol for spermatid isolation from bull testis using a discontinuous Percoll gradient and 10 μm mesh cell strainers. Next, we established a stage-specific separation strategy based on DNA content, size, and granularity using flow cytometry to distinguish round and elongating/elongated spermatids suitable for molecular analysis. Morphological assessment confirmed that 72.5% of isolated cells were at the spermatid stage, supported by a high haploidy rate, spermatid-specific transcript expression (, , , ), and SPERT protein detection. Viability assays showed that spermatids maintained intact DNA at 0 and 24 h at 4°C and 37°C, though mitochondrial activity and ROS levels increased over time, suggesting oxidative stress. When spermatids were injected into oocytes (n = 82), only 13.4% formed two pronuclei, whereas 46.3% exhibited a single pronucleus and a condensed chromatin spot, indicating incomplete activation or fertilization failure. This work contributes to refining bovine intracytoplasmic injection protocols. Future applications of this approach, particularly if functional spermatids can be derived from spermatogonia or embryonic cells, could help shorten the generational interval in cattle breeding. - Source: PubMed
Publication date: 2025/05/16
Pasquariello RolandoDi Filippo FrancescaBonumallu Sai Kamal NagFagali Franchi FernandaPistucci RamonaFranciosi FedericaLodde ValentinaIannuzzi AlessandraLuciano Alberto MariaBrevini Tiziana A LGandolfi Fulvio - Biological parenthood in men with azoospermia can be achieved only by successful surgical spermatozoa retrieval and utilising these spermatozoa to perform intracytoplasmic spermatozoon injection. Spermatozoa retrieval from testes (SPERT) involves removing seminiferous tubules in an attempt to retrieve spermatozoa in men with non-obstructive azoospermia (NOA). Testicular volume and serum follicle-stimulating hormone (FSH) levels are considered to be markers for predicting the success of spermatozoa retrieval from the testes. - Source: PubMed
Publication date: 2025/03/29
Thanikachalam PuvithraRavikuppan GayatriArunkumar SowbarnikaPandiyan RadhaNatarajan Pandiyan