Ask about this productRelated genes to: SPATA22 antibody
- Gene:
- SPATA22 NIH gene
- Name:
- spermatogenesis associated 22
- Previous symbol:
- -
- Synonyms:
- NYD-SP20
- Chromosome:
- 17p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 2005-12-19
- Date modifiied:
- 2019-03-19
Related products to: SPATA22 antibody
Related articles to: SPATA22 antibody
- The spotted scat (Scatophagus argus), an economically valuable aquaculture species in southern China, exhibits a pronounced sexual dimorphism in growth performance, with females growing significantly faster than males. Nevertheless, progress in sex-controlled breeding remains limited due to limited understanding of its sex determination and differentiation mechanisms. To investigate sex-biased gene expression in this species, a comprehensive transcriptomic analysis was performed. A total of 62 transcriptomic libraries were analyzed, comprising 18 newly sequenced libraries derived from female gill, muscle, stomach, heart, and adipose tissue, and male adipose tissue, integrated with 44 publicly available libraries covering the brain, pituitary gland, liver, kidney, gonads, and other tissues. The analysis identified 33,214 unigenes, including 8958 novel genes, with 1724 receiving functional annotation. Principal component analysis (PCA) and heatmap clustering revealed distinct expression profiles in gonadal (ovary and testis) and somatic tissues. Differential expression analysis identified 2951 ovary-highly expressed and 1660 testis-highly expressed genes. Functional annotation revealed genes essential for folliculogenesis, spermatogenesis, and meiosis, including figla, gdf9, mos, amhr2, spata22, and dmc1. Additionally, 23 ovary-specific and 75 testis-specific genes were identified. KEGG enrichment analysis revealed significantly enriched pathways in the gonads, including oocyte meiosis, cell cycle, and DNA replication. RT-PCR and qRT-PCR validations confirmed the RNA-seq results, demonstrating consistent tissue-specific expression patterns of these genes. These findings advance the understanding of the molecular mechanisms of sex differentiation and gametogenesis in spotted scat and provided a foundation for studies of reproductive regulation and gonadal development in this species. - Source: PubMed
Publication date: 2026/01/28
Lin XinghuaWu TianliJiang DongnengShi HongjuanTian ChangxuChen HuapuLi GuangliDeng Siping - Genome-wide association studies (GWASs) have been performed on multiple epigenetic clocks to understand the genetic underpinnings of epigenetic age acceleration (EAA). To explore ethnic differences and individual variability in Asian populations, we conducted a GWAS of EAA using multiple epigenetic clocks in the Korean population. Genome-wide single-nucleotide polymorphism (SNP) and methylation data were obtained from 1962 Korean individuals. Five epigenetic clocks (Hannum, DNAm PhenoAge, GrimAge, Zhang2019, and Horvath-Skin&Blood) were considered. GWASs, as well as gene-based, pathway enrichment, quantitative real-time polymerase chain reaction, and quantitative trait loci analyses, were performed to investigate the biological mechanisms. Pleiotropy analyses and drug repurposing were used to explore clinical implications. Six SNPs were independently associated with EAAs (P < 5 × 10). The DNAm PhenoAge-EAA was associated with ASPA and SPATA22 (P < 2.66 × 10), involved in Canavan disease and apoptosis, and the Zhang2019 was associated with ENO2, GNB3, USP5, TPI1, and CDCA3 (P < 2.66 × 10), which are implicated in monosaccharide catabolism. Particularly, the expression levels of SPATA22, ENO2, USP5, and TPI1 increased in senescent cells. EAA-associated genes showed strong affinities with GW0742, a peroxisome proliferator-activated receptor-β/δ (PPAR-β/δ) agonist. In addition, the Zhang2019-EAA-associated SNP showed pleiotropy in liver function biomarkers, including iron and bilirubin. We identified six Asian-specific EAA-associated genetic variants, including five novel SNPs. The novel SNPs, which are rare in European populations, could implicate ancestry-related genetic differences in epigenetic aging. Furthermore, the genetic implications of the DNAm PhenoAge-EAA and Zhang2019-EAA on apoptosis and liver function may contribute to a better understanding of EAA in East Asian populations. - Source: PubMed
Publication date: 2025/09/15
Oh KwangyeonYun DabinYang SoyounYuk MinjuLee ChanggyuKim BoyoungHeo SuyeonDong QianWang ZhaomingPark HanseulSong Nan - The male hybrid of cattle and yak is sterile, while the female is fertile. This is caused by aberrations with the process of spermatogenesis. SPATA22 plays a critical role in the pairing of homologous chromosomes, synapsis, DNA repair, and meiotic recombination; dysregulation of which can disrupt these processes, resulting in meiotic arrest and male sterility in mammals. The expression and molecular characterization of have not been studied in the sterile hybrid cattle-yak. This study isolated, sequenced and characterised the coding sequence of the gene of the cattle-yak, and compared the relative mRNA and protein expression. There was a remarkable reduction in mRNA and protein expression in the cattle-yak compared to the yak and cattle. The SPATA22 protein was located in the adluminal compartment of the tubules. Thirteen mutation sites consisting of ten amino changes and 3 insertions were found in the cattle-yak sequence. The cattle-yak protein sequence contains motifs which are potential binding sites for genes that play a crucial role in meiotic recombination and DNA repair. Histological evaluation revealed no advanced-stage germ cells, except a few spermatogonia, spermatocytes and Sertoli cells. These findings give an insight into the role of in the hybrid sterility of cattle-yak and pave the way for more future research into genetic engineering involving the construction of a functional gene copy into the genome of sterile male cattle-yak to restore normal spermatogenesis in the sterile hybrid. This will help improve desirable traits in the yak through hybridization. - Source: PubMed
Publication date: 2025/06/17
Niayale RobertCui YanYu Si-JiuWang LibinZhang JianLiao BoLi ShijieZhang QianXu HongweiZhao PengfeiWei Pengqiang - MEIOB and SPATA22 are gonad-specific proteins that function in meiosis recombination. Mutations in these two proteins cause oligospermia or azoospermia in human males. It has been reported that the heterodimer composed of MEIOB and SPATA22 recognizes and binds to the single-strand DNA (ssDNA) protected by the replication protein A (RPA) complex to promote DNA damage repair during homologous recombination. However, the amino acid sequences of the two proteins are inconsistent in humans and rodents, which leads to functional differences in meiosis. In this study, human-derived MEIOB (hMEIOB) and SPATA22 (hSPATA22) are expressed and purified for electrophoretic mobility shift assay (EMSA), magnetic tweezer (MT) assay and bio-layer interferometry (BLI) assay to analyze the ssDNA binding patterns. The results show that hMEIOB has low ssDNA-binding affinity and stability alone, but hSPATA22 binds to ssDNA faster and more stably and promotes ssDNA condensation. Strong binding affinity and stability to ssDNA are present when the hMEIOB-hSPATA22 heterodimer is formed. Moreover, we find that multiple hMEIOB-hSPATA22 heterodimers spontaneously aggregate . hRPA complex weakens the binding affinity of hMEIOB, hSPATA22 and hMEIOB-hSPATA22 heterodimer to ssDNA, and it can also bind to hSPATA22 and hMEIOB-hSPATA22 heterodimer , which might be related to the proven function of RPA complex to protect ssDNA and recruit proteins related to DNA damage repair during meiosis. Overall, this study is the first time to elucidate the binding patterns of the hMEIOB and hSPATA22 to ssDNA , and to verify the relationship between the RPA complex and meiosis-related proteins, MEIOB and SPATA22, from single-molecule perspective. - Source: PubMed
Xu YatingQu WeiZhou ErchiSun QiGong WeihaoXu LeiLei YaokeJia ZhangyingShi HanqingZhang XinghuaLuo Mengcheng - Non-obstructive azoospermia (NOA) is the most severe form of human male infertility, and the genetic causes of NOA with meiotic arrest remain largely unclear. In this study, we identified novel compound heterozygous MEIOB variants (c.814C > T: p.R272X and c.976G > A: p.A326T) and a previously undescribed homozygous non-canonical splicing variant of MEIOB (c.528 + 3A > C) in two NOA-affected individuals from two irrelevant Chinese families. MEIOB missense variant (p.A326T) significantly reduced protein abundance and nonsense variant (p.R272X) produced a truncated protein. Both of two variants impaired the MEIOB-SPATA22 interaction. The MEIOB non-canonical splicing variant resulted in whole Exon 6 skipping by minigene assay, which was predicted to produce a frameshift truncated protein (p.S111Rfs*32). Histological and immunostaining analysis indicated that both patients exhibited a similar phenotype as we previously reported in Meiob mutant mice, that is, absence of spermatids in seminiferous tubules and meiotic arrest. Our study identified three novel pathogenic variants of MEIOB in NOA patients, extending the mutation spectrum of the MEIOB and highlighting the contribution of meiotic recombination related genes in human fertility. - Source: PubMed
Publication date: 2023/09/16
Zhu XiaoyuHu KaiqinCheng HuiruWu HuanLi KuokuoGao YangLv MingrongXu ChuanGeng HaoShen QunshanCao YunxiaHe XiaojinTang DongdongGuo Rui