Ask about this productRelated genes to: SNX7 antibody
- Gene:
- SNX7 NIH gene
- Name:
- sorting nexin 7
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 1p21.3
- Locus Type:
- gene with protein product
- Date approved:
- 2001-04-10
- Date modifiied:
- 2016-10-05
Related products to: SNX7 antibody
Related articles to: SNX7 antibody
- Establishment of canine acanthomatous ameloblastoma (CAA) cell lines has profound importance in pre-clinical in vitro testing. The goal of this study was to develop, authenticate, and characterize three CAA cell lines. Cell lines were developed with standard cell culture techniques from dogs with naturally occurring CAA. Each cell line was authenticated as canine with a validated PCR panel. Epithelial cell origin was confirmed with pan-CK flow cytometry. Next generation DNA and RNA sequencing characterized features of the cell lines and compared them to the parent tumor. We confirmed that all cell lines maintained mutations seen in the parent tumors including a missense HRAS mutation and RTK-RAS pathway upregulation across all samples. Other common mutations (2/3 cell lines and their parent tumors) included ADGRA3, DNAH7, F10, KIAA1671, OGFR, SLC6A17, SNX7, SPTBN5, TENM4, and YEATS2. We compared the transcriptional profiles from our parent tumors and established cell lines to historical transcriptomic analysis of CAA and healthy gingiva. We confirmed the same canonical CAA molecular features of primary tumors across studies with distinct clustering from healthy gingiva. Further, we documented that the cell lines maintained upregulated genes that are seen within in vivo CAA across both studies including upregulated GPC4 and ETV5 as well as gene sets associated with the presence of epithelial-mesenchymal transformation, KRAS, Pi3K-AKT signaling, and hedgehog signaling pathways. Thorough characterization of the mutational and transcriptional profiles of the established cell lines, especially in context to how they differ from the parent tumors, sets the platform for translational in vitro testing. - Source: PubMed
Publication date: 2026/07/21
Goldschmidt StephanieVapniarsky NataliaMcPherson John DRivas IrisLy ChristineMorales AbrahamYork DanielRebhun RobertSoltero-Rivera Maria - Glaucoma trabecular meshwork (GTM) cells cultured in vitro retain many characteristics of their in situ phenotype. Here, we used isobaric tandem mass tags (TMTpro) to label peptides from glaucomatous and non-glaucomatous TM (NTM) cells to identify differentially regulated proteins. Confluent NTM (n = 5) and GTM (n = 5) cells were lysed, proteins were trypsin digested, and peptides were labeled with 18-plex TMTpro. TMT-labeled peptides were fractionated on an Orbitrap Fusion mass spectrometer and data were processed using the PAW/Comet pipeline and EdgeR with Benjami–Hochberg multiple correction testing. Isobaric multiplexed quantitative proteomics identified 206 proteins that were significantly (FDR < 0.1) upregulated in GTM cells, 42 proteins that were downregulated, with 5270 non-candidates. Significant regulated pathways included extracellular matrix (DCN, COL4A1, CHI3L1), Wnt signaling (FZD1, FZD7, GSK3B), cytoskeletal regulation (ROCK2, MSN, TPM2, VIM, NF2), protein degradation (USP9X, LAMP1, SYNV1, UBE2L3), and nuclear proteins (LMNA, DFFA, CHMP3, RAD21). Western immunoblotting studies confirmed the TMTpro data. Immunofluorescence showed that the SNX7-stained nucleoli of GTM cells were significantly (p < 0.05) larger, and the DIAPH2 immunostaining was more distended into the cytosol than in NTM cells. This study identified many significantly regulated proteins in cultured GTM cells, and the results revealed several new avenues for developing clinical therapies for glaucoma patients. - Source: PubMed
Publication date: 2026/03/18
Holden PaulSun Ying YingZientek KeithWilmarth Phillip AReddy Ashok PKeller Kate E - Radiotherapy is a primary treatment for intermediate and advanced cervical cancer (CC). Resistance to radiotherapy is a principal reason for treatment failure in synchronous applications, yet the molecular mechanisms remain poorly understood. Identifying reliable prognostic markers to predict and evaluate patient outcomes is essential for advancing therapeutic strategies. This study aims to address this need by developing a prognostic prediction model for concurrent radiotherapy in CC, utilizing both single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing data. - Source: PubMed
Publication date: 2025/10/31
Yang SiqiLiu LitingSu QiuyueWang JiananXia JingqiZhao XinyaoSun YajuanYang Shanshan - BACKGROUND: Prostate cancer (PC) is a major health concern among men worldwide, yet its underlying molecular mechanisms remain incompletely understood. Identifying key regulatory genes and signaling pathways involved in PC progression is essential for improving diagnosis and developing targeted therapies. METHODS: Prostate adenocarcinoma (PRAD) samples from The Cancer Genome Atlas (TCGA) and the GSE46602 dataset were analyzed using bioinformatics techniques in order to determine the hub gene linked to PC. Through cell experiments, we studied the effects of SNX7 and its related genes on PC cell proliferation, migration, invasion, and autophagy-related protein binding. Additionally, we looked into the connection between SNX7 expression and other immune cells. RESULTS: We discovered that a favorable prognosis for patients with PC was linked to increased expression of SNX7. The function of SNX7 as a tumor suppressor gene in PC was further demonstrated by in vitro experiments, and its overexpression may successfully stop PC cell proliferation. CFLIP was positively correlated with SNX7, and its overexpression significantly reduced PC cell viability, migration, and invasion. Rescue experiments showed that SNX7 overexpression reversed the proliferative and invasive effects induced by CFLIP knockdown. Additionally, CFLIP knockdown enhanced the interaction between ATG3 and LC3A, whereas overexpression of SNX7 or CFLIP weakened this binding. SNX7 was also found to be associated with various immune cells, hinting at a prospective immunomodulatory role in PC. CONCLUSION: The research results showed that SNX7 activates the expression of CFLIP, inhibited the binding of ATG3 and LC3, and inhibited the occurrence of autophagy, emphasizing the potential diagnostic value of SNX7 in PC. - Source: PubMed
Publication date: 2025/08/29
Qin LiangYang FanGuo ZhuifengLu XuweiWu JiawenJiang DongzhenYang Ning - Glioblastoma is a highly aggressive and invasive brain tumor with an extremely poor prognosis. The aims of the present study are to investigate the pathogenesis of glioblastoma and identify potential therapeutic targets. - Source: PubMed
Publication date: 2024/12/28
Zhang YuanlongChen BinghongLiu RenfuMei WenzhongLin Yuanxiang