Ask about this productRelated genes to: SLC5A8 antibody
- Gene:
- SLC5A8 NIH gene
- Name:
- solute carrier family 5 member 8
- Previous symbol:
- -
- Synonyms:
- AIT
- Chromosome:
- 12q23.1-q23.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-05-12
- Date modifiied:
- 2018-02-13
Related products to: SLC5A8 antibody
Related articles to: SLC5A8 antibody
- SLC5A8 is a protein coded by the gene, and has been proposed as a tumor suppressor and iodide transporter. Its expression is reduced in papillary thyroid carcinoma (PTC), yet the mechanisms underlying this phenomenon are largely unknown. We hypothesized that expression in PTC is reduced by microRNAs and can be modulated by their inhibition. We used real-time PCR to analyze the expression of and the microRNAs of interest in a set of 49 PTC/normal tissue pairs. We used an in silico approach to identify microRNAs upregulated in PTC and putatively binding to the transcript. Luciferase assays were performed to confirm the direct binding of synthetic microRNAs to the 3'UTR of . Subsequently, using mir-expressing plasmids and microRNA sponges, including a microRNA sponge designed to simultaneously inhibit three selected microRNAs, we checked the impact of the modulation of microRNAs on endogenous . Finally, we investigated if modulation of SLC5A8 induces changes in transcriptomes. We confirmed the downregulation of in PTC. In silico analysis revealed microRNAs potentially targeting . Luciferase assay confirmed direct binding between the 3'UTR of and miR-181a-5p, miR-182-5p, and miR-494-3p. MiR-181a-5p and miR-182-5p were upregulated in PTC. In HEK293 cell lines, transfection with mir-181a- and mir-182-expressing plasmids decreased endogenous mRNA, while silencing of miR-181a-5p, miR-182-5p, miR-494-3p, and all three microRNAs simultaneously increased expression; however, only simultaneous inhibition was able to induce changes visible for SLC5A8 protein. Changes in expression did not alter the whole transcriptome significantly. This study shows microRNA-dependent regulation of expression and underlines the potential effectiveness of simultaneous inhibition of a few microRNAs to derepress their common target. - Source: PubMed
Publication date: 2025/08/15
Gierlikowski WojciechGrzędzicka JowitaKonieczek KatarzynaKotlarek-Łysakowska Marta - The goal of this study was to evaluate effects of short-term acute salinity challenges on the hemocyte physiology in northern quahogs, Mercenaria mercenaria, an important aquaculture species in the U.S. The objectives were to: 1) challenge adult northern quahogs with salinities of 5, 15, 25 (control), 35, and 45 ppt; 2) evaluate cellular responses, including hemocyte concentration, viability, phagocytosis rate, ROS production, and lysosomal presence at 12 h, 24 h, 48 h, 72 h, 144 h, and 21 days post-challenge; and 3) evaluate the molecular response of hemocytes after 72 h challenge. M. mercenaria at 5, 15, 25, 35 ppt exposure showed no mortality, while at 45 ppt showed a 100 % mortality at day 7, reflecting better tolerance to hyposalinity. Changes of hemolymph osmolality at different salinities and exposure periods indicated M. mercenaria equilibrates with the environmental seawater within its tolerance range (15-35 ppt) as an osmoconformer. Hemocyte concentration increased significantly at 5, 15, and 45 ppt stress. Hemocyte immune functions exhibited a varied pattern in response to different salinity levels, enabling them to resolve stresses. The RNAseq of hemocytes revealed DEGs in response to salinity challenges, specifically enrichment of amino acid transporters (SLC2A5, SLC6A1, SLC5A8, SLC1A3, SLC25A38), immune response (CHAC2, MGST3, IRF1, and IRFD2) and cell signaling (Wnt, TRAIL-activated and Toll) pathway in hyper/hypo salinity groups, indicated their importance in salinity stress responses. This study provided critical insights into the cellular and molecular responses of M. mercenaria hemocytes to salinity challenges. - Source: PubMed
Publication date: 2025/07/31
Zeng YangqingZeng XianyuanYee Jayme CYang Huiping - To date, there is no effective treatment for glioblastoma (GBM). This study aimed to compare the effectiveness of sodium dichloroacetate (NaDCA), a valproic acid and NaDCA combination (VPA-NaDCA), or temozolomide (TMZ) on U87 and T98G cell tumors on the chick embryo chorioallantoic membrane (CAM), and on the expression of proliferating cell nuclear antigen (PCNA), polycomb inhibitory complex catalytic subunit 2 (EZH2), and gene-encoded p53 protein (p53) in tumors on the CAM, and (gene encoding Na-K-2Cl (NKCC1) co-tarnsporter), (gene encoding K-Cl (KCC2) co-transporter), (gene encoding Na-dependent monocarboxylate transporter) and (gene encoding the E-cadherin protein) and (gene encoding the N-cadherin protein) in cells. VPA-NaDCA and TMZ reduced the invasion of U87 and T98G tumors, as well as the expression of PCNA and EZH2 in the tumor. TMZ reduced p53 expression in tumors from both cell lines, whereas VPA-NaDCA did not affect the expression of this marker. VPA-NaDCA, but not TMZ, reduced expression in T98G cells. However, VPA-NaDCA and TMZ did not affect expression in U87 cells. VPA-NaDCA increased expression only in U87 cells, and TMZ did not affect gene expression in either cell line. Only VPA-NaDCA increased expression and decreased expression in T98G cells, whereas TMZ had no effect on gene expression in the study cells. This study demonstrated that VPA-NaDCA exhibits a more effective anticancer effect than NaDCA. The data suggest that VPA-NaDCA has a more effective impact than TMZ; however, the effect of investigational medicines on carcinogenesis varies depending on the cell line. The study of the efficacy of drugs used to treat tumors on the CAM and cells demonstrates that it is essential to assess the effectiveness of treatment, which should be personalized, before administering chemotherapy. - Source: PubMed
Publication date: 2025/07/15
Skredėnienė RūtaStakišaitis DonatasValančiūtė AngelijaBalnytė Ingrida - Solute carrier family-5 member-8 (SLC5A8) serves as a plasma membrane transporter for monocarboxylates, such as lactate, butyrate, pyruvate, acetate, propionate, nicotinate, and β-hydroxybutyrate. SLC5A8 can suppress colorectal cancer (CRC), and its tumor-suppressive function is mainly associated with butyrate, propionate, and pyruvate, which inhibit histone deacetylase. Although SLC5A8 is an important tumor suppressor, the impact of variants on its tumor-suppressive function have not been reported. In this study, we investigated the effects of missense variants on the expression and tumor-suppressive function of the transporter using various in vitro assays. - Source: PubMed
Publication date: 2025/07/14
Ha Seung YeonSong Hyo SookKim Jin-YoungChoi Youn-HeeChoi Ji Ha - Intestinal diseases in nursery pigs harm health and performance and drive antimicrobial resistance. This study evaluated whether early probiotic inoculation helps piglets to cope with weaning-related gut challenges. The probiotic, containing , , subsp. , and , was given orally to newborn piglets daily until day 4 and then every other day until weaning at day 28 (at 4 × 10 CFU/dose). The control piglets received a placebo. The results showed that the probiotic pigs had reduced fecal alpha-diversity on day 7 but greater Shannon diversity on day 28 (feces) and day 23 (intestinal contents) compared to those of the control pigs. Beta-diversity analysis showed microbial differences between the groups on day 35. Most zOTUs (zero-radius operational taxonomic units) found to significantly differentiate the two treatment groups were found pre weaning. , , as well as were significantly more abundant in the feces of the probiotic pigs more than once. The probiotic pigs had higher expression levels of mucin 2 (MUC2); solute carrier family 5, member 8 (SLC5A8); and interleukin 8 (IL-8) post weaning. In the early post-weaning period, the probiotic pigs had less diarrhea as well as lower cadaverine levels in digesta than the control pigs. In conclusion, early probiotic inoculation may induce lasting immunomodulation via microbial antigen changes, enhancing resilience during challenges, like weaning. Notably, the effects persisted beyond weaning and probiotic cessation. - Source: PubMed
Publication date: 2025/05/31
Hansen Lea Hübertz BirchLauridsen CharlotteNielsen BeaJørgensen LisbethSchönherz AnnaCanibe Nuria