Ask about this productRelated genes to: RPS8 antibody
- Gene:
- RPS8 NIH gene
- Name:
- ribosomal protein S8
- Previous symbol:
- -
- Synonyms:
- S8
- Chromosome:
- 1p34.1
- Locus Type:
- gene with protein product
- Date approved:
- 1992-07-20
- Date modifiied:
- 2016-10-05
Related products to: RPS8 antibody
Related articles to: RPS8 antibody
- BACKGROUND Acute myocardial infarction (AMI) reflects inherited susceptibility and inflammatory remodeling, but the cellular contexts linking genetic risk to disease remain unclear. MATERIAL AND METHODS We integrated a meta-transcriptome-wide association study (TWAS) with a human cardiac single-nucleus RNA-sequencing atlas contained 11 individuals (5 AMI and 6 donor) to identify genetics-informed cellular programs. Composite program states were defined by global score quartiles. A fixed 5-gene panel was evaluated for nucleus-level endocardial low-transcriptional-state (Endo_LTS) vs endocardial high-transcriptional-state (Endo_HTS) discrimination within the AMI endocardium using 5-fold leave-1-patient-out cross-validation. Functional follow-up used CRIM1 silencing in hypoxia-treated human induced pluripotent stem cell (hiPSC)-derived endocardial endothelial-like cells and complementary peripheral blood analyses. RESULTS The endocardium exhibited the most prominent infarction-associated increase in TWAS-anchored program activity, with expansion of program-high states and higher CytoTRACE scores. A consensus 5-gene panel (RPS8, PLEC, CFDP1, CRIM1, TNS2) was identified. Among 2163 AMI endocardial nuclei from 5 patients, the state classifier included 364 Endo_LTS and 751 Endo_HTS nuclei; 1048 Endo_MTS nuclei were excluded. Pooled out-of-fold ROC-AUCs ranged from 0.665 to 0.831. The panel also showed discriminatory value in an independent peripheral-blood AMI-vs-control cohort. CRIM1 was prioritized as a candidate linked to the remodeling program. CRIM1 silencing attenuated ACTA2/alpha-SMA, vimentin, LDHA, CCL2, and VEGFA and partially restored CD31, whereas TGF-ß remained elevated. CONCLUSIONS These findings identify a genetics-informed endocardial inflammatory remodeling state in AMI and define a 5-gene surrogate of its activated state. CRIM1 is prioritized as a candidate linked to selected inflammatory, metabolic, and structural outputs. Persistent TGF-b elevation after CRIM1 silencing argues against a simple linear regulatory model and indicates that further mechanistic validation is required. - Source: PubMed
Publication date: 2026/08/28
Ruan RuyiXu XiaofengLiu QuanXiang FuLuo LanShi LijieYang Quankun - The genus Zehneria (Cucurbitaceae) presents significant taxonomic challenges due to morphological similarities among species and a lack of resolution in molecular studies. In this study, we sequenced and assembled the chloroplast genomes of seven African Zehneria species collected from Kenya, complementing a previously published accession (Zehneria sp. MZ427944.1), to establish a comprehensive plastome-based framework for the genus. The plastomes ranged from 157,150 to 157,365 bp and exhibited a conserved quadripartite structure, with 130-132 annotated genes, including 86-87 protein-coding genes, 36-37 tRNAs, and 8 rRNAs. Structural variation, particularly in non-coding regions and inverted repeat boundaries, was observed, with eight hypervariable loci providing potential targets for molecular marker development. Simple sequence repeat (SSR) analysis revealed a predominance of A/T mononucleotides across species. Codon usage analysis revealed a strong AT-bias across all species, with codons ending in A or U accounting for 72.2% of synonymous codon preferences. Selective pressure analysis identified eight protein-coding genes with Ka/Ks ratios exceeding 1.0, including psaJ and rps8, proposed as candidate molecular barcodes pending further validation. Phylogenomic analysis of 79 protein-coding genes strongly supported the monophyly of Zehneria (ML bootstrap = 96-100%; PP = 1.0), with one node showing slightly reduced support (BS = 96%), and enhanced resolution of interspecific relationships compared to prior studies using fewer loci. This work offers novel insights into genomic diversity and evolutionary history of sampled Zehneria species, providing a critical molecular resource for future taxonomic and phylogenetic studies within Cucurbitaceae. Broader taxon sampling, particularly from Asian lineages, will be necessary to fully resolve interspecific relationships across the genus. - Source: PubMed
Publication date: 2026/08/18
Karendi Michael GichuruNdungu Caroline NjambiMbadi Elijah MkalaWanga Vincent OkeloNyamvula Harriet MelanyKaigongi Magrate MHu Guang-Wan - Oxidized low-density lipoprotein (ox-LDL) and inflammation cause endothelial dysfunction in coronary heart disease (CHD). Although ribosomal protein S5 (RPS5) has been linked to cardiovascular disease, the mechanism by which it functions in the endothelium and its relationship to NF-κB signaling remain unknown. RPS5 was chosen as the hub gene for CHD after differential expression analysis was done on the GSE68506 dataset. The human coronary artery endothelial cells (HCAECs) injury model was established by ox-LDL. In vitro experiments were performed to evaluate the changes in RPS5 expression, apoptosis, inflammatory markers, vascular protective factors, cell proliferation, and Endothelial-to-Mesenchymal Transition (EndMT). Combining the dual luciferase reporter gene assay and the NF-κB nuclear translocation immunofluorescence assay, the effect of RPS5 on the NF-κB pathway was evaluated. Seven ribosomal genes (RPL11, RPL8, RPS13, RPS20, RPS3, RPS5, RPS8) were downregulated in coronary artery disease (CAD) samples in the GSE68506 dataset. In vitro, ox-LDL injury similarly reduced RPS5 expression in HCAECs. Functionally, RPS5 knockdown inhibits cell proliferation and enhances apoptosis and EndMT, while RPS5 overexpression has the opposite effect. Mechanistically, RPS5 overexpression diminished NF-κB nuclear accumulation, lowered κB-WT reporter activity, and reduced the phosphorylation levels of IKKα/β, IκBα, and p65. It also reduced pro-inflammatory mediators and decreased VEGF and HSP70. The pathway dependency was confirmed when LPS-induced NF-κB activation reversed these effects. Through the NF-κB signaling pathway, RPS5 plays a crucial role in controlling inflammation, apoptosis, EndMT, and vascular protection factors in injured HCAECs. Endothelial damage in CHD may be treatable by focusing on the RPS5-NF-κB axis. - Source: PubMed
Publication date: 2026/07/13
Han SuxiaLi WanqiuHuang YanXu YifeiSong HaihanQi Weigang - The reliability and accuracy of relative quantification of transcripts through quantitative real-time PCR (qPCR) is dependent on the use of stable reference genes for normalization. However, the expression stability of various reference genes may differ across the tissues and patho-physiological conditions. The present study investigated the stability of multiple candidate reference genes in Japanese quail (Coturnix japonica) embryos, subjected to embryonic thermal conditioning (ETC). Fertilized quail eggs (n = 200) were incubated under standard conditions and heat exposure (ETC) of incubated eggs was undertaken at 39.5 °C for 10 h daily in the embryonic days 6-8 and 12-14, maintaining constant humidity (55%). Four embryos were randomly selected from each group for experimentation. The expression levels of eight candidate reference genes i.e., 18 S rRNA, β-ACTIN, 28 S rRNA, HPRT1, GAPDH, PGK1, RPS8, and TBP were assessed across four tissues (liver, intestine, brain, and heart) under ETC. Gene expression stability was assessed using five different algorithms: geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder (Comprehensive gene stability). Variations in gene stability were evident across different tissues and algorithms. The HPRT1, GAPDH and RPS8 emerged as the most stable reference genes for qPCR analyses in liver tissue, making them suitable internal controls. Similarly, PGK1, RPS8, and HPRT1 genes exhibited higher expression stability in intestine. On the other hand, TBP and HPRT1 were identified as the most reliable reference genes for normalization in heart tissue, whereas HPRT1 and GAPDH were found to be the most reliable reference genes in brain tissue. On considering all tissues combined (liver, intestine, brain, and heart), TBP and RPS8 emerged as the most suitable gene set for normalization. This present study provides crucial insights into selecting appropriate reference genes for relative expression quantification studies in quail embryos under heat treatment (ETC), thereby improving the reliability and accuracy of qPCR data in avian developmental and stress physiology research. - Source: PubMed
Publication date: 2026/07/01
Ansari Mohd MatinMartina KKolluri GauthamTyagi Jagbir SinghRokade JaydipBhutekar PrashantNaim AdnanGangwar MunishAhmad Sheikh Firdous - Aphids are serious sap-sucking pests that damage okra () and reduce yield through feeding and virus transmission. Conventional insecticides create resistance, environmental pollution, and health hazards. Eco-friendly nanotechnology offers a new direction for insect control using safe, plant-based materials. - Source: PubMed
Publication date: 2026/05/31
Habeeb Akmam Ali