Ask about this productRelated genes to: RPS13 antibody
- Gene:
- RPS13 NIH gene
- Name:
- ribosomal protein S13
- Previous symbol:
- -
- Synonyms:
- S13
- Chromosome:
- 11p15.1
- Locus Type:
- gene with protein product
- Date approved:
- 1993-11-05
- Date modifiied:
- 2016-10-05
Related products to: RPS13 antibody
Related articles to: RPS13 antibody
- The present study investigated the effects of different intensities of blue light on papillae number growth and associated molecular mechanisms in the sea cucumber Apostichopus japonicus. The sea cucumber were exposed for 60 days under three blue light intensities (1500 lx, 3000 lx, and 4500 lx). Papillae numbers and body weight were measured regularly. Transcriptomic sequencing was performed to analyze gene expression differences in spine tissues. The results showed that the number of papillae was significantly highest in the sea cucumbers exposed to 1500 lx. Body weight growth was not significantly affected by any light intensity treatment. Compared with the control group, the 1500 lx group showed differentially expressed genes (DEGs) significantly enriched in pathways including the ras signaling pathway, ascorbate and aldarate metabolism, and fatty acid elongation. Seven key genes potentially related to the growth of papillae number were identified: Survivin, PHB2, SoxB, traf6, TRPML3, TGF-β1, and Rps13. In contrast, compared with the 1500 lx group, both the 3000 lx group and the 4500 lx group showed differentially expressed genes (DEGs) mainly enriched in pathways including axon regeneration, regulation of actin cytoskeleton, fc gamma R-mediated phagocytosis, and chemokine signaling. Six genes potentially inhibiting the growth of papillae number were identified: CRK, Smad4, Rac1, Arf6, ASAP2, and Wnt7. In conclusion, the light intensity of 1500 lx effectively increased the papillae number in A. japonicus. This study provides molecular evidence for enhancing papillae number in cultured sea cucumbers through light intensity regulation. - Source: PubMed
Publication date: 2026/04/22
Li WeiyanLiu ZiyuDeng YajieLiu JiaqiYu JingeXiao HaoranTian FenglinHan LingshuZhao ChongDing Jun - Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors worldwide. This necessitates the development of innovative drugs with high efficiency, low toxicity, and good tolerance. Bitter melon extract has been reported to have potent anticancer activity against OSCC. We evaluated the effects of nine triterpenoids from bitter melon extract on OSCC using cell counting kit-8 (CCK-8) proliferation and Transwell migration assays. Among the nine triterpenoids, momordicine I (MI) exhibited the strongest anticancer activity against OSCC. Animal experiments also showed that MI inhibited OSCC cell growth in vivo. Additionally, MI decreased the mitochondrial membrane potential and promoted apoptosis in OSCC. RNA-sequencing (RNA-seq) analysis revealed that MI induced an unfolded protein response (UPR) and endoplasmic reticulum (ER) stress, which was confirmed by western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cellular thermal shift assay (CETSA) and mass spectrometry (MS) analysis, combined with molecular docking, identified ribosomal proteins (ribosomal protein L7 (RPL7), RPL11, RPL12, RPL18, RPL30, RPL38, RPS13, and RPS25) as MI targets. By targeting ribosomal proteins, MI likely disrupts ribosome-mediated protein folding, leading to the UPR and ER stress. In summary, MI targets ribosomal proteins to induce ER stress and inhibit OSCC, highlighting its therapeutic potential. - Source: PubMed
Publication date: 2025/12/22
Kong JianluZhu ZiyuHu YijieZhou SiyiGu TianyiShen XiaoWang HuimingYu MengfeiLiu Yu - Graptopetalum paraguayense is a perennial succulent plant with ornamental, ecological, and medicinal value. Here, we present the first complete mitogenome of G. paraguayense, assembled as a circular molecule of 242,059 bp with a GC content of 43.65%. The genome contains 50 genes, including 31 protein-coding genes (PCGs), 13 tRNAs, 3 rRNAs, and 3 pseudogenes. A total of 599 RNA editing sites were identified, with a predominant effect of altering amino acid hydrophobicity (47.41% were hydrophilic-to-hydrophobic conversions). Codon usage bias analysis revealed a preference for GCU (Ala), CGA (Arg), and UUA (Leu), with the stop codon UAA exhibiting the highest RSCU value (1.94). A total of 122 repetitive sequences were identified, comprising 59 simple sequence repeats (SSRs), 1 tandem repeat, and 62 dispersed repeats. Evolutionary analysis indicated positive selection on ccmB and nad7 genes, while the majority of PCGs were under purifying selection. The mitogenome of G. paraguayense shared 57.28% sequence similarity with that of Sedum plumbizincicola. We also found evidence of chloroplast-to-mitochondrial DNA transfer, involving genes such as psaC, ndhE, ndhG, ndhI, ndhA, and ndhH. Comparative analyses identified eleven divergent hotspot regions: atp9, atp8, rpl5, cox2, ccmFn, rps7, ccmC, ccmFc, mttB, nad6, and rps13. Phylogenetic analysis confirmed the placement of G. paraguayense within the Acre clade of Crassulaceae, showing a sister relationship with S. plumbizincicola. Our study not only provides the first mitochondrial genomic resource for G. paraguayense but also reveals potential adaptive evolution through positive selection and interorganellar gene transfer, offering new perspectives on mitogenome plasticity in succulent plants. - Source: PubMed
Publication date: 2026/01/03
Zhou XueRen QingmingLin ChuqiLi ZhiruiZhou LinXiong FeiDu Xi - Previous studies indicate that stroke recovery is worse in patients with prior COVID-19, suggesting persistent biological perturbations. We investigated lingering renin-angiotensin system (RAS) and inflammatory alterations after COVID-19 to uncover mechanisms driving impaired ischemic stroke recovery. We conducted a prospective observational cohort study comparing clinical and molecular profiles of ischemic stroke patients with and without recent COVID-19 infection to age-matched healthy controls. Plasma angiotensin-(1-7), angiotensin II, and soluble ACE2 were quantified, high-throughput proteomic profiling was performed using the Olink® Explore platform, and RNA sequencing was conducted to identify molecular mechanisms related to COVID-19-associated stroke and stroke outcomes (90-day modified Rankin Scale (mRS)). A total of 189 participants (38 COVID-19-associated stroke, 77 non-COVID-19 stroke, and 74 healthy controls) were enrolled. COVID-19-associated stroke patients exhibited significantly higher proportions of cryptogenic strokes (21.1 % vs 10.4 %), earlier hospital presentation (mean 185 vs 310 min), greater use of endovascular thrombectomy (97.4 % vs. 52.6 %), yet poorer functional outcomes (mRS ≥3) at 3 months (73 % vs. 47 %, all p < 0.05). Both stroke groups showed elevated angiotensin-(1-7) levels compared to controls, but angiotensin II levels were notably higher only in non-COVID-19 stroke patients. Proteomic analysis revealed sustained elevation of interferon-gamma (IFN-γ) signaling in COVID-19-associated stroke patients. Reduced AKT3 levels emerged as a significant predictor of poor outcomes, independent of renin-angiotensin biomarkers (adjusted OR 0.40; 95 % CI 0.16-0.94). Stroke patients with low AKT3 levels and poor outcomes exhibited significant upregulation of ribosomal proteins (RPS15, RPS4X, RPS7, RPS18, RPSA, RPS27A, RPS13, RPS23), reflecting heightened translational stress. Persistent RAS and inflammatory dysregulation following COVID-19 may contribute to worse stroke recovery. Future studies should validate and investigate the therapeutic implications of these findings. - Source: PubMed
Publication date: 2025/11/20
Quek Amy May LinTeng OoieanPark Ju-HeaEr Bernadette Guek ChengLim Erle Chuen HianSeet Raymond Chee Seong - Epidemiological and experimental evidence indicate that hyperglycemia, transient hyperinsulinemia and insulin-like growth factor-1 promote an aggressive phenotype of breast tumor cells during diabetes mellitus. The present study evaluated the effect of high glucose (HG) concentration (30 mmol/l) on cisplatin sensitivity and gene expression in MDA-MB-231 triple-negative breast cancer (TNBC)-derived cells. Cisplatin cytotoxicity was assessed by the MTT assay and was attenuated by HG. This effect was accompanied by reduced caspase-3 activation, indicating impaired apoptosis. Differentially expressed genes between HG and normal glucose (5.6 mmol/l) conditions were analyzed by microarray. The upregulation of tetraspanin 1 () and frizzled 3 () was validated by reverse transcription-quantitative PCR. HG promotes cisplatin chemoresistance by preventing apoptosis. Additionally, HG altered the expression of genes involved in glucose, glycerophospholipid, purine and pyrimidine metabolism. Regulatory pathways affected included Wnt/β-catenin, p53 network, NF-κB survival and DNA damage response. Candidate genes potentially associated with HG-induced chemoresistance included and . Survival analysis using the KM-Plotter platform revealed that and amphiregulin expression were significantly associated with poor prognosis. By contrast, amyloid precursor protein () and N-cadherin () showed non-significant trends. Coexpression analyses demonstrated that and were positively correlated with This suggests they may contribute to tumor aggressiveness as part of -driven regulatory networks, rather than as independent prognostic markers. Collectively, the present findings demonstrated that HG promotes cisplatin resistance and aggressive features in TNBC cells through multiple metabolic and signaling pathways. Notably, these include the Wnt/β-catenin axis. Candidate biomarkers with potential prognostic and therapeutic relevance in diabetic breast cancer (BC) were also identified. - Source: PubMed
Publication date: 2025/10/02
Viedma-Rodríguez Araceli RubíMartínez-Hernández María GuadalupeFlores-López Luis AntonioVelázquez-Flores Miguel ÁngelEsparza-Garrido Ruth RuizPrado-Baeza Javier RodrigoBaiza-Gutman Luis Arturo