Ask about this productRelated genes to: RNF181 antibody
- Gene:
- RNF181 NIH gene
- Name:
- ring finger protein 181
- Previous symbol:
- -
- Synonyms:
- HSPC238
- Chromosome:
- 2p11.2
- Locus Type:
- gene with protein product
- Date approved:
- 2007-06-19
- Date modifiied:
- 2014-11-19
Related products to: RNF181 antibody
Related articles to: RNF181 antibody
- PIWI-interacting RNAs (piRNAs), a class of 23-31 nucleotide non-coding RNAs, are known for silencing transposons and endogenous retroviruses that reside in animal genomes. However, the mechanisms by which host piRNAs affect exogenous viral infections, particularly those by DNA viruses, remain poorly understood. Here, we demonstrated that infection by Bombyx mori nucleopolyhedrovirus (BmNPV), a large DNA virus, induced significant upregulation of silkworm host piR-bmo-796514, which facilitated viral proliferation by suppressing the expression of E3 ubiquitin ligase RNF181. We further revealed that RNF181 exerted antiviral activity through ubiquitin-mediated degradation of Integrin α2b-like, a cellular membrane protein that interacted with viral GP64 protein to mediate BmNPV entry. This study unveiled a previously unrecognized regulatory axis connecting host derived piRNAs with exogenous DNA virus infection, providing further mechanistic insights into the modulation of exogenous viral pathogenesis through the reprogramming of the piRNA pathway. Our findings not only advance the understanding of the immune escape mechanism of exogenous viruses but also provide new insights for the development of oligonucleotide antiviral drugs that target proviral piRNAs. - Source: PubMed
Publication date: 2026/01/06
Xia JunmingFei ShigangLuo WenjieZhou MingyangKong YibingHuang YiguiSwevers LucFeng Min - Mounting evidence links tumor heterogeneity with gut microbiome characteristics. Akkermansia muciniphila's outer-membrane protein Amuc_1100 is emerging as a microbe-derived enhancer of antitumor immunity that boosts antitumor immunity. Considering that RNF181 may be involved in regulating the degradation of tumor immune-related proteins, we hypothesize that RNF181 may play a critical role in modulating lung adenocarcinoma (LUAD) immune microenvironment. We co-cultured LUAD cells treated with Amuc_1100 with CD8T cells to evaluate their cytotoxicity and proliferation ability (LDH detection, CFSE method), cytokine levels (ELISA), and cell surface PDL1 expression (flow cytometry). Next, the acetylation level of RNF181 and the expression of downstream substrate ATG7 in LUAD cells treated with Amuc_1100 were analyzed (qPCR, WB) to verify the ubiquitination degradation effect of RNF181 on ATG7 (WB), and its effect on PD-L1 mRNA expression was detected (qPCR). Finally, Amuc_1100 was injected into mice with normal immune function to analyze the CD3/CD8T cell ratio (flow cytometry) and changes in immune-related cytokine levels (ELISA) in LUAD tissue. LUAD cells treated with Amuc_1100 significantly increased the acetylation level of RNF181, thereby increasing the mRNA and protein expression of RNF181. Meanwhile, RNF181 can also promote the degradation of ATG7 protein through ubiquitination modification. Through these regulatory effects, Amuc_1100 can activate the immune level of LUAD and enhance its immune response ability. Amuc_1100 enhances RNF181 expression through acetylation of its promoter region. RNF181 promotes ubiquitination and degradation of ATG7, leading to downregulated PD-L1 and enhanced CD8 T cell-mediated antitumor activity. - Source: PubMed
Publication date: 2025/09/18
Xu YufenQian TingtingPan HuanGu YuyangXu MaoyiBai ChunshengChen Wenyu - Amyotrophic lateral sclerosis (ALS), a devastating neurodegenerative disease, poses a significant challenge for targeted treatment development. Accurate prediction of its progression is crucial for this endeavor. - Source: PubMed
Publication date: 2025/06/30
Qin HaoshenHussain LalLiu ZiangYan XuAwwad Fuad AButt Faisal MehmoodSalaria Umair AhmadIsmail Emad A A - Nasopharyngeal carcinoma (NPC) is an aggressive malignancy with a poor prognosis. Ubiquitination is a complex post translational modification involved in cancer progression. However, ubiquitination related genes (URGs) in immunotherapy of NPC remains largely unexplored. - Source: PubMed
Publication date: 2025/06/25
Deng HaiyanZhang JuanChen ShuaijunLiang TingfengHu XueyongLi JingHe YongYu FengYu Chaosheng - Dysregulated ERα signaling is responsible for endocrine resistance and eventual relapse in patients with estrogen receptor-positive (ER) breast cancer. Thus, identifying novel ERα regulators is necessary to fully understand the mechanisms of endocrine resistance. Here, we identified circRNA-SFMBT2 to be highly expressed in ER breast cancer cells in comparison to ER cells and found that high circRNA-SFMBT2 levels were related to larger tumor size and poor prognosis in patients with ER breast cancer. In vitro and in vivo experiments confirmed that the circRNA-SFMBT2 level was positively correlated with the ERα protein level, implying a regulatory role for circRNA-SFMBT2 in ERα signaling. Moreover, we found that circRNA-SFMBT2 biogenesis could be facilitated via RNA-binding protein quaking (QKI), and biologically elevated circRNA-SFMBT2 expression promoted cell growth and tamoxifen resistance in ER breast cancer. Mechanistically, circRNA-SFMBT2 exhibits a specific tertiary structure that endows it with a high binding affinity for ERα and allows it to interact with the AF2 and DBD domains of ERα, enforcing recruitment of RNF181 to the AF1 domain of ERα. Furthermore, the circRNA-SFMBT2/RNF181 axis differentially regulated K48-linked and K63-linked ubiquitination of ERα to enhance ERα stability, resulting in increased expression of ERα target genes and tumor progression. In summary, circRNA-SFMBT2 is an important regulator of ERα signaling, and antagonizing circRNA-SFMBT2 expression may constitute a potential therapeutic strategy for breast cancer. - Source: PubMed
Publication date: 2023/07/31
Li ZhengLi YamingHan DianwenWang XiaolongLi ChenChen TongLi WenhaoLiang YiranLuo DanChen BingWang LijuanZhao WenjingYang Qifeng