Ask about this productRelated genes to: RAB3B antibody
- Gene:
- RAB3B NIH gene
- Name:
- RAB3B, member RAS oncogene family
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 1p32.3
- Locus Type:
- gene with protein product
- Date approved:
- 1989-06-30
- Date modifiied:
- 2016-10-05
Related products to: RAB3B antibody
Related articles to: RAB3B antibody
- Premature rupture of membranes (PROM) is a major cause of preterm birth and neonatal mortality. Tight junctions are critical for maintaining fetal membrane barrier integrity. Ras-related protein Rab-3B (RAB3B), a key regulator of vesicle trafficking, may influence barrier homeostasis; however, its role in PROM remains unclear. - Source: PubMed
Publication date: 2026/03/07
Su ShuguangYe QiantaoChen FeilongTian QinGan CaixiaLi Yanqiu - Semantic variant of primary progressive aphasia is a clinical subtype of frontotemporal lobar degeneration and is marked by TDP-43 subtype C pathology (FTLD-TDP C). It is a sporadic disease, yet has a strikingly homogeneous clinicopathological presentation, suggesting a common pathophysiology. The aim of this study was to discover dysregulated pathways in FTLD-TDP C through transcriptomics of the temporal cortex, its most affected region. Bulk RNA sequencing was conducted on temporal cortices of a post-mortem cohort of 18 FTLD-TDP C patients and 23 sex- and age-matched controls. Differential expression and functional analyses were run to detect differentially expressed genes with FDR<0.05 (DEG) and functionally annotate them. We assessed enrichment of TARDBP's protein interactors and RNA targets in DEG. Our findings were compared to other published RNA sequencing data of tauopathies (Alzheimer's dementia, progressive supranuclear palsy and FTLD with MAPT), FTLD-TDP (subtypes A&B) and available proteomics of this cohort. Furthermore, we performed weighted gene co-expression network analysis (WGCNA). We adjusted for differences in cell type composition between cases and controls using cell deconvolution, and removed genes dysregulated in temporal cortices of other datasets. In DEG of FTLD-TDP we focused on enrichment of synaptic processes using SynGO. We found upregulation of damage response, cell structure, RNA splicing processes and downregulation of synaptic processes in 6322 DEG and five disease-related WGCNA modules. TARDBP-related genes were enriched in DEG. Additionally, transmembrane transport across the neurovascular unit was dysregulated. After cell deconvolution and removal of common tau-genes, postsynaptic processes remained dysregulated, specifically gene ontology terms 'modulation of chemical synaptic transmission' and 'neurotransmitter receptor localisation to postsynaptic specialisation membrane'. We found eleven synaptic FTLD-TDP C-specific genes affected on both RNA- and protein-level in the temporal cortex, which were involved in synaptic adhesion (CADM1, NCAN), signal transmission (COMT, RGS144, SLC1A2, TUBB2B) and synaptic plasticity (BEGAIN, ITPKA, LRFN1, RAB3B, SYNPO). In conclusion, a wide range of processes were dysregulated on RNA-level in the temporal cortex of FTLD-TDP C, including commonly affected processes in neurodegeneration, such as structural cell alterations. Dysregulation of TARDBP-related genes and RNA splicing has also been observed in other TDP-43 proteinopathies. Importantly, we found that postsynaptic processes were downregulated in FTLD-TDP C, after removing tauopathy-related genes and after cell deconvolution. In particular, assembly of receptors at the postsynaptic membrane and synaptic signal transmission were affected, both on RNA and protein level. Future research on these pathways could elucidate distinct pathophysiological mechanisms and guide targeted clinical approaches. - Source: PubMed
Publication date: 2026/03/06
Rajicic AnaMol Merel OMelhem ShamiramKisic Helenavan Swieten John CSeelaar Harrovan Rooij Jeroen G J - The diagnostic rate of prostate cancer (PCa) remains suboptimal, particularly for patients whose serum prostate-specific antigen (PSA) concentration is less than 10 ng/mL. Single-cell sequencing, machine learning, and Mendelian randomization have emerged in recent years as excellent methods for identifying tumour markers. We identified 3 genes (RAB3B, SPON2, and SLC4A4) through single-cell sequencing and a machine learning screen. Among them, RAB3B (Ras-related protein, Rab-3B) had a higher area under the diagnostic curve for PSA < 10 ng/mL PCa (0.763) than the other two genes did. Mendelian randomization analysis revealed that the occurrence of PCa led to an increase in circulating levels of the RAB3B protein. Western blot and immunohistochemical analyses demonstrated that RAB3B was overexpressed in PCa tumour. Subsequently, elevated levels of RAB3B were detected in the culture medium of PCa cells. Furthermore, urinary RAB3B protein levels were significantly increased in PCa patients. For patients with PSA levels < 10 ng/mL, urinary RAB3B exhibited an area under the receiver operating characteristic curve (AUC) of 0.733 for PCa diagnosis, outperforming both PSA concentration (AUC = 0.682) and age (AUC = 0.651). Notably, the AUC of the multivariate model urine RAB3B + PSA + Age is 0.8. GSEA at al analysis and functional experiments, including gain-of-function and loss-of-function studies revealed that RAB3B promotes the proliferation and migration of PCa cells. In summary, urinary RAB3B, discovered through a multiomics approach, is a promising biomarker that is complementary to serum PSA, and together these factors can improve diagnostic decision-making in the PSA grey zone. - Source: PubMed
Publication date: 2025/12/19
Zhao ChongyuDeng GengguoChen JunliangLi HaomingFan RongFu ZhonglingYe DongmingLai CaiyongDi Jinming - This study explored the molecular mechanism of the hypothalamus-pituitary-gonadal (HPG) axis on the regulation of brooding behavior and laying performance of Wanxi white geese (WWG). The transcriptome of the hypothalamus, pituitary, and ovarian tissues of laying and brooding WWG was sequenced to identify genes and long noncoding RNAs (lncRNAs) that may be important in regulating the egg-laying performance and broodiness behavior of WWG. - Source: PubMed
Publication date: 2025/10/22
Li XiaojinHou MengmengWang YuhuaWu FouTong XinweiXie FeiJiang ChangshengJin MengmengRen ManLi Shenghe - DENN/MADD (mitogen-activated protein kinase-activating death domain), a differentially expressed in normal and neoplastic cells (DENN) domain-containing protein functions in membrane trafficking. DENN domain-bearing proteins have guanine nucleotide exchange factor activity toward Rab GTPases. Here, we identify Rab GTPase substrates for DENN/MADD using a cell-based assay involving DENN domain-mediated recruitment of Rab substrates to mitochondria. We confirmed known interactions of DENN/MADD with Rab3A, Rab3B, Rab3C, Rab3D, and Rab27B and identified four new potential substrates, Rab8B, Rab15, Rab26, and Rab37, results confirmed with biochemical experiments. Mutations in the DENN domain of DENN/MADD result in diverse pathophysiological manifestations, ranging from predominant neurological dysfunction to a multisystem disorder. Structural analysis using AlphaFold suggested that these mutations affect DENN/MADD's interaction with Rab GTPases. Introducing such mutations into DENN/MADD's DENN domain influenced the mitochondrial recruitment of Rabs. This study identifies new DENN/MADD protein interactions and cellular pathways, the disruption of which results in human disorders. - Source: PubMed
Publication date: 2025/08/12
Khan MaleehaKumar RahulTrempe Jean-FrançoisFrancis VincentBanks EmilyAyoubi RihamLuna Luis AguileraMcPherson Peter S