Ask about this productRelated genes to: PTPN23 antibody
- Gene:
- PTPN23 NIH gene
- Name:
- protein tyrosine phosphatase non-receptor type 23
- Previous symbol:
- -
- Synonyms:
- DKFZP564F0923, KIAA1471, HD-PTP
- Chromosome:
- 3p21.31
- Locus Type:
- gene with protein product
- Date approved:
- 2001-02-15
- Date modifiied:
- 2019-02-14
Related products to: PTPN23 antibody
Related articles to: PTPN23 antibody
- Secreted proteins are essential for processes like immune responses, cellular communication, and extracellular matrix remodeling. Once synthesized and processed at the Golgi, some of these proteins are packaged for delivery to the plasma membrane. While this transport and sorting rely on complex molecular machinery, the precise mechanisms remain unclear. In this study, we affinity-isolated and analyzed post-Golgi carriers by mass spectrometry. Candidate machinery was subsequently assessed in a pooled CRISPR-KO screen. This led to the identification of a rich set of new genes functionally important for Golgi-to-plasma membrane delivery including PTPN23, a component of the endosomal sorting complex required for transport (ESCRT) complex. Depletion of PTPN23, as well as the ESCRT subunits CHMP1 and VPS4, disrupts tubule fission from the trans-Golgi, impairing cargo delivery to the surface. Furthermore, the loss of PTPN23 also prevents the constitutive secretion of soluble cargoes, and of endogenous hormones and antibodies in specialized cells. We propose that PTPN23 is essential for secretion from the trans-Golgi. - Source: PubMed
Publication date: 2026/03/18
Stalder DanièlePereira Conceiçãovan den Boomen Dick J HAdarska PetiaMenon DilipFazakerley Daniel JBottanelli FrancescaLehner Paul JGershlick David C - Improving fibre quality traits is a key objective in alpaca breeding programmes. A total of 630 alpaca individuals managed at the Pacomarca experimental farm located in Puno, Perú, and subjected to selection for fibre quality traits were genotyped using the Affymetrix Custom Alpaca genotyping array (76 508 SNPs). Two tests, the integrative Haplotype Score (iHS) and the number of segregating Sites by Length (nSL), aimed at identifying incomplete sweeps, were applied on all sample genotypes to identify selective candidate genomic regions spanning genes putatively involved in fibre production. The Cross-Population Extended Haplotype Homozygosity (XP-EHH) statistic was applied to two divergent subpopulations included in the total genotyped population: one less selected (reference; 49 individuals) and one highly selected (target; 127 individuals) to assess if some of the selective sweeps identified with both iHS and nSL were fixed in the target subpopulation. A total of 544 single-nucleotide polymorphisms (SNPs) were identified as candidate selective signals using both the iHS and nSL tests. Furthermore, 27 SNPs were identified as surrounding selective signals and further considered as candidate selective signals as well. A total of 509 candidate selective genomic regions covering 14.6 Mb of the alpaca genome were constructed. Gene-annotation enrichment analyses enabled to identify 293 candidate genes in these selective candidate genomic regions. Up to 11 candidate genes (e.g. PTPRN, FRK, PTPN23, and PTPRT) were associated with Gene Ontology (GO) terms related to phosphorylation and dephosphorylation and 28 candidate genes (e.g. FOXP1, MITF, RNF10, and ZNF664) with GO terms related to RNA polymerase activity. These genes have been reported to play a crucial role in the development of animal hair follicles, as well as in regulating hair growth and quality across different livestock species. Functional annotation conducted allowed to identify four significantly enriched functional term clusters including a total of 32 candidate genes. These functional clusters may be involved in the modulation of fibre growth and quality as well as skin and hair follicle development. Moreover, enrichment analyses within candidate selective genomic regions identified with XP-EHH allowed to identify 54 candidate genes, 10 of which were also identified using both the iHS and nSL tests. However, no functional clusters were identified. The results presented suggest that the genomes analysed were shaped by directional selection for fibre quality traits, involving multiple chromosomal areas of the alpaca genome. This fits well with traits expected to be mainly controlled by genes of additive effect. - Source: PubMed
Publication date: 2025/10/04
Arias K DMore MGoyache FCruz ABurgos AGutiérrez GCervantes IGutiérrez J P - The clearance mechanisms for ubiquitinated protein aggregates, such as MAPT/tau in neurodegenerative diseases, remain incompletely understood, particularly regarding the role of microautophagy. To identify mediators of this process, we performed an unbiased genome-wide CRISPR knockout screen using cells propagating MAPT/tau repeat domain (MAPT/tauRD) aggregates. This screen identified the ESCRT-I complex and the accessory protein PTPN23 as essential for the clearance of ubiquitinated MAPT/tauRD aggregates via a microautophagy-dependent pathway, operating independently of macroautophagy and chaperone-mediated autophagy. We designate this pathway "microaggrephagy". Mechanistically, microaggrephagy involves the recognition of polyubiquitinated aggregates by the ESCRT-I subunit TSG101, with PTPN23 acting as an adaptor bridging ESCRT-I and ESCRT-III to facilitate microautophagic engulfment. Furthermore, a disease-associated mutation in the ESCRT-I component UBAP1 disrupts its interaction with PTPN23 and impairs MAPT/tau clearance, implicating dysfunction of this pathway in neurodegenerative pathogenesis. These findings establish microaggrephagy as a distinct cellular mechanism for degrading pathological protein aggregates, provide a molecular basis for its function, and suggest potential therapeutic targets for proteinopathies. - Source: PubMed
Publication date: 2025/07/06
Hirayama ShoshiroMurata Shigeo - Osteosarcoma is a highly aggressive bone tumor that primarily affects adolescents and young adults, posing significant challenges in therapeutic efficacy, prognostic assessment, and treatment strategies. This study investigates the oncogenic and immune regulatory roles of the PTPN family in osteosarcoma using a comprehensive multi-omics approach. We utilized transcriptomic data, single-cell RNA sequencing (scRNA-seq), and clinical information obtained from publicly available databases. Dimensionality reduction and clustering techniques were employed to subclassify immune cells and analyze the tumor microenvironment characteristics. We identified prognostic genes associated with the PTPN family and stratified osteosarcoma cases into distinct molecular subtypes using consensus clustering. A random forest model revealed that the PTPN family has a significant impact on prognosis and modulates key oncogenic pathways. Furthermore, we analyzed the role of the PTPN family in regulating immune cells and selected PTPN23 for experimental validation. This research not only enhances prognostic assessments in osteosarcoma but also establishes a foundation for personalized therapeutic interventions. - Source: PubMed
Publication date: 2025/06/26
Long ChanghaiMa BiaoZhong XingshunZou MingzhiLi KaiLiu Sijing - In the genetic breeding research of dairy goats, traditional genotyping methods have limitations, and existing goat chips have shortcomings in functional loci and other aspects, which cannot meet the precise genetic analysis needs of dairy goats. Genotyping by Target Sequencing (GBTS) in the new generation of sequencing technology provides the possibility to solve these problems. - Source: PubMed
Publication date: 2025/04/15
Zhao JianqingWang YalingKamalibieke JiayidaerGong PingZhang FuhongShi HuaipingWang WeiLuo Jun