Ask about this productRelated genes to: PRPF3 antibody
- Gene:
- PRPF3 NIH gene
- Name:
- pre-mRNA processing factor 3
- Previous symbol:
- RP18
- Synonyms:
- Prp3, hPrp3, SNRNP90
- Chromosome:
- 1q21.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-12-05
- Date modifiied:
- 2016-10-05
Related products to: PRPF3 antibody
Related articles to: PRPF3 antibody
- Lung adenocarcinoma (LUAD) is a leading cause of cancer mortality, necessitating the identification of robust biomarkers and a deeper understanding of its molecular underpinnings. This study is aimed at screening for potential LUAD biomarkers and characterizing their biological functions. Using an integrative computational framework, we combined multitranscriptomic data analysis with three machine learning algorithms (LASSO, SVM-RFE, and random forest) to identify a consensus seven-gene signature (TTC13, TAF1D, ZNF587, PRPF3, LINC01355, TARBP1, and CCNL2). A classifier based on this signature achieved exceptional diagnostic accuracy (AUC = 0.972), with TAF1D identified as the most influential predictor via SHAP analysis. TAF1D was significantly upregulated in tumors, correlated with an immunosuppressive microenvironment, and promoted cancer cell proliferation by regulating cell cycle and immune-related pathways. Critically, TAF1D exhibited significant spatial heterogeneity in expression across different samples and tissue regions, suggesting it may exert region-specific biological functions within the tumor. In conclusion, our work defines a validated gene signature for LUAD, nominating TAF1D as a key oncogenic driver and promising candidate for diagnostic and therapeutic development. - Source: PubMed
Publication date: 2026/04/11
Ding LanXu QingmeiLiu DongdongWu JingyuCai XufanXu FeiqiMa ShuhanWang HaitaoShi Yanyan - The progression of spermatogenesis is under dynamic transcriptional regulation. As a subunit of the transcription-export complex 2 (TREX-2), PCI domain-containing protein 2 (PCID2), participates in RNA processing. However, the physiological functions of PCID2 in spermatogenesis remain poorly understood. Here, we generate germline conditional knockout (Pcid2-SKO) mice using Stra8-Cre, and it is found that Pcid2-SKO mice are infertile, exhibit extensive germ cell apoptosis, impaired spermatogonial differentiation, and failure of meiosis initiation. Single-cell transcriptome analysis reveals developmental arrest at the transition from type A to type B spermatogonia in Pcid2-SKO mice. Gene Set Enrichment Analysis (GSEA) demonstrates a significant decrease in the enrichment of mRNA splicing pathway in Pcid2-SKO germ cells. IP-MS results indicate candidate proteins interacting with PCID2 are significantly enriched in RNA splicing pathway. Co-IP results indicate that PCID2 interacts with SNRPG, hnRNPH1 and SF3B1 to modulate alternative splicing in germ cells. Combining RNA sequencing and PCR identifies four key genes (Prpf3, Nek3, Dvl2, and Slc30a9) as splicing targets of PCID2. Collectively, PCID2 is essential for normal spermatogenesis and male fertility by regulating the alternative splicing (AS) of genes critical for cell cycle progression, spliceosome assembly, and mitochondrial homeostasis. This study provides novel insights into the molecular mechanisms underlying spermatogenesis and highlights the importance of AS in germ cell development. - Source: PubMed
Publication date: 2026/01/13
Zhu FeiyinZhang YingXi YuGong ChenjiaTang YanlinChen YidongYan LiyingQiao JieLiu Qiang - Small nuclear RNAs (snRNAs) combine with specific proteins to generate small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. U4 snRNA forms a duplex with U6 and, together with U5, contributes to the tri-snRNP spliceosomal complex. Variants in RNU4-2, which encodes U4, have recently been implicated in neurodevelopmental disorders. Here we show that heterozygous inherited and de novo variants in RNU4-2 and in four RNU6 paralogs (RNU6-1, RNU6-2, RNU6-8 and RNU6-9), which encode U6, recur in individuals with nonsyndromic retinitis pigmentosa (RP), a genetic disorder causing progressive blindness. These variants cluster within the three-way junction of the U4/U6 duplex, a site that interacts with tri-snRNP splicing factors also known to cause RP (PRPF3, PRPF8, PRPF31), and seem to affect snRNP biogenesis. Based on our cohort, deleterious variants in RNU4-2 and RNU6 paralogs may explain up to ~1.4% of otherwise undiagnosed RP cases. This study highlights the contribution of noncoding RNA genes to Mendelian disease and reveals pleiotropy in RNU4-2, where distinct variants underlie neurodevelopmental disorder and retinal degeneration. - Source: PubMed
Publication date: 2026/01/09
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Testa FrancescoKarali MarianthiBoccia RosaPisani DanilaDamiano LucianaNicolò AntonioMadonna EmanueleDe Rosa LuigiColucci RaffaellaDe Benedictis AntonellaDi Iorio ValentinaMelillo PaoloBanfi SandroSimonelli Francesca - Pre-mRNA processing factor 4 (PRPF4), a core protein of U4/U6 small nuclear ribonucleoproteins (snRNPs), is crucial for maintaining their structure by interacting with PRPF3 and Cyclophilin H. Beyond its role in splicing, PRPF4 has been implicated in cell survival, apoptosis, and oncogenesis. Although PRPF4 mutations have been associated with retinitis pigmentosa, its role in glioblastoma (GBM) remains unclear. This study aimed to investigate the function of PRPF4 in GBM progression and its potential as a therapeutic target. - Source: PubMed
Kim WansooPark SongHan Se-HyeonKim Hee-YeonLee Seoung-WooKim DaehwanJang SoyoungPark Jin-KyuHan Jee EunKim ChoonokCho JaelimSeah EthanLee JiyeonRyoo Zae YoungChoi Seong-Kyoon