Ask about this productRelated genes to: PRKDC antibody
- Gene:
- PRKDC NIH gene
- Name:
- protein kinase, DNA-activated, catalytic subunit
- Previous symbol:
- HYRC, HYRC1
- Synonyms:
- DNPK1, p350, DNAPK, XRCC7, DNA-PKcs, DNAPKc, DNA-PKC, p460
- Chromosome:
- 8q11.21
- Locus Type:
- gene with protein product
- Date approved:
- 1993-11-09
- Date modifiied:
- 2019-04-23
Related products to: PRKDC antibody
Related articles to: PRKDC antibody
- Engineered plasma cells (ePCs) offer a durable strategy for delivery of therapeutic antibodies, but standard immunodeficient mouse models lack human immune factors critical for PC survival and function. We utilized a humanized mouse model in which NOD.Cg- /SzJ (NSG) mice were engrafted with human CD34 stem cells as recipients for infusions with autologous ePCs. In this setting, ePCs localized to PC niches and stably secreted antibodies for over 3 months. To improve the selection of antibodies for secretion, we developed a B cell receptor surface display screen that identified candidate antibody sequences with high secretion potential. An anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody (clone 297) selected by this method showed robust secretion both and , and serum from ePC-engrafted mice potently neutralized SARS-CoV-2 pseudovirus. Together, these findings establish a physiologically relevant model for testing human ePCs, and offer a generalizable strategy for optimizing antibody selection to support long-term therapeutic delivery. - Source: PubMed
Publication date: 2026/01/09
Hill Tyler FHelmers Anna EYu-Hong Cheng ReneSingh SwatiHomad Leah JNarvekar ParnalAsher Gregory DCamp Nathan DSuchland Emma ROtt Andee RHale MalikaThouvenel Chris DStoffers Claire MLachkar StefanMcGuire Andrew TRawlings David JJames Richard G - : Stromal remodeling in the tumor microenvironment contributes to multiple myeloma (MM) progression and drug resistance, but the extracellular mediators that drive these changes remain incompletely defined. Extracellular enolase-1 (ENO1), including membrane-associated and secreted forms, has been implicated in tumor progression; however, its role in cancer-associated fibroblast (CAF)-associated stromal reprogramming in MM is unclear. : The effects of extracellular ENO1 on stromal activation and tumor-supportive functions were examined in MM using MM-bone marrow stromal cell (BMSC) co-cultures, lactate production and viability assays, immunoblotting, cytokine analyses, and a subcutaneous xenograft model of bortezomib (BTZ)-resistant MM in male 6-7-week-old NOD.Cg-Prkdc Il2rg/Vst (NPG) mice. HuL001, an anti-ENO1 monoclonal antibody, was used to evaluate the therapeutic relevance of extracellular ENO1 targeting. : Extracellular ENO1 promoted fibroblast activation protein expression through plasmin-mediated transforming growth factor-β (TGF-β) activation and induced a CAF-associated stromal phenotype marked by enhanced glycolytic activity and increased secretion of tumor-promoting cytokines in MM-BMSC co-cultures. HuL001 suppressed these ENO1-driven effects. HuL001-pretreated stromal cells also exhibited reduced tumor-supportive activity in a BTZ-resistant MM xenograft model. In addition, HuL001 combined with lenalidomide overcame BTZ resistance in MM. : Extracellular ENO1 drives CAF-associated stromal reprogramming in the MM microenvironment through the ENO1/plasminogen/plasmin/TGF-β axis. Therapeutic targeting of extracellular ENO1 with HuL001 may disrupt these tumor-supportive stromal activities and help overcome drug resistance in MM. - Source: PubMed
Publication date: 2026/05/02
Chung I-CheChuang Tung-YuehKo Yu-TungChen Mao-LinHsu Po-YangHuang Wei-ChingYuan Ta-Tung - Spatial transcriptomics has emerged as a transformative approach for in situ mapping of cellular heterogeneity and interactions, yet existing methods often compromise throughput, cost and tissue coverage. Here we introduce Imaging Reconstruction using Indexed Sequencing (IRISeq): an optics-free, cost-effective platform that leverages spatial interaction mapping by indexed sequencing to profile tissues at adjustable sizes and resolutions (5-50 µm). We applied IRISeq to map gene expression across more than 70 coronal sections from both adult and aged mouse brains, including wild-type and two lymphocyte-deficient models (Rag1 and Prkdc mutants) and generated more than 460,000 spatial transcriptome profiles. Our integrated analysis with 783,264 single-cell transcriptomes revealed region-specific aging signatures that are lymphocyte dependent, notably a downregulation of interferon signaling and inflammation in ventricular regions upon lymphocyte depletion, alongside mutant-specific upregulation of senescence pathways. Furthermore, lymphocyte deficiency was linked to preserved abundance of ependymal cells that line the brain's ventricles and to distinct microglial state dynamics, highlighting a key role for lymphocytes in driving inflammatory processes during brain aging. Overall, IRISeq provides a high-throughput and cost-effective solution for spatially resolved transcriptomic profiling, opening new avenues for elucidating region-specific cellular mechanisms underlying aging and identifying potential therapeutic targets to preserve brain homeostasis. - Source: PubMed
Publication date: 2026/05/12
Abdulraouf AbdulraoufJiang WeirongZhang ZehaoXu ZihanLu ZiyuMerlinsky TiffanyLiao AndrewDoymaz AhmetIsakov SamuelRaihan TanvirZhou WeiCao Junyue - Tuberculosis is an infectious disease caused by (), which poses a notable threat to human health. The present review aims to explore the application of humanized mice in the study of infections. Due to differences in immune responses between mice and humans, humanized mice with human immune systems have been developed as models to characterize human immune responses to . The present review searched for research on humanized mice and tuberculosis in Web of Science and PubMed using the keywords 'humanized', 'mice' or 'mouse' and 'tuberculosis', and summarized the findings. Humanized non‑obese diabetic (NOD).Cg‑Rag1Il2rg and NOD.Cg‑PrkdcIl2rg mice have the potential to accelerate the screening of vaccine candidates, therapeutic regimens and the 'bench to bedside' translation process. New therapies, such as IgG1 P1AM25 in humanized Fcγ receptor mice and phage DS6A in humanized NOD.Cg‑Prkdc Il2rg Tg(cytomegalovirus‑interleukin‑3, granulocyte‑macrophage colony‑stimulating factor and KIT ligand)1Eav/MloySzJ mice, may have potential for treating tuberculosis. The humanized bone marrow‑liver‑thymus and human leukocyte antigens transgenic mouse models are effective tools for studying the co‑infection of and human immunodeficiency virus (HIV). The present review highlights the key role of humanized mouse models in advancing the understanding of infection, including host‑pathogen interactions, immune evasion mechanisms, vaccine development, therapeutic interventions and co‑infection with HIV. In conclusion, humanized mice provide a powerful platform for bridging the gap between preclinical research and clinical tuberculosis therapeutics. - Source: PubMed
Publication date: 2026/05/08
Han BinghuaLiu FangLong JinzhaoWang JiongjiongCui YangeYang Haiyan - Sinonasal cancers are malignant neoplasms arising from the nasal cavity and paranasal sinuses, including squamous cell carcinoma (SCC), adenocarcinoma, and undifferentiated carcinoma. Due to their rarity, comprehensive genomic data remain limited. Treatments include surgery, radiation, and chemotherapy, with ongoing trials investigating agents like cetuximab, cisplatin, and Tazemetostat. - Source: PubMed
Publication date: 2025/07/02
Bitar GabrielHsia BeauAlshaka SaifValencia Bastien AKim JeehoSato MarikoCrawford JohnLevy Michael LPolster SeanPatel Vijay A