Ask about this productRelated genes to: PI4KB antibody
- Gene:
- PI4KB NIH gene
- Name:
- phosphatidylinositol 4-kinase beta
- Previous symbol:
- PIK4CB
- Synonyms:
- PI4K-BETA, pi4K92
- Chromosome:
- 1q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 1998-04-07
- Date modifiied:
- 2016-10-05
Related products to: PI4KB antibody
Related articles to: PI4KB antibody
- Although KRAS-driven tumors exhibit elevated macroautophagy/autophagy, the extent to which this process diverges from canonical regulatory pathways has not been well characterized. In a recent study published in Cell Research, Wang et al. unveil a novel form of non-canonical autophagy driven by oncogenic RAS mutations, which they termed RAS-induced non-canonical autophagy via ATG8ylation (RINCAA). This pathway operates through a unique MAPK/p38-ULK1-PI4KB axis, diverging significantly from canonical starvation-induced autophagy. The research not only elucidates a new regulatory mechanism but also identifies a potential, highly specific therapeutic target for RAS-mutant cancers.: PI4KB, phosphatidylinositol 4-kinase beta; PtdIns4P, phosphatidylinositol-4-phosphate; RINCAA, RAS-induced non-canonical autophagy via Atg8ylation; ULK1, unc-51 like autophagy activating kinase 1; WIPI2, WD repeat domain phosphoinositide-interacting protein 2. - Source: PubMed
Publication date: 2025/12/25
Wen XinKlionsky Daniel J - Paraptosis is a non-apoptotic form of programmed cell death, distinct from classical apoptosis in morphology and mechanism. It has been implicated in tumor resistance and immune microenvironment remodeling, but its role in breast cancer (BC) remains unclear. We classified patients into two subtypes based on the expression of paraptosis-related genes. Then, we systematically analyzed the prognosis and tumor microenvironment (TME) associated with these subtypes. In addition, we developed a risk score, named the paraptosis-related risk score (PRRS). We comprehensively analyzed the correlation of paraptosis with BC prognosis, TME, immune score, and drug sensitivity. Then, we performed in vitro experiments to verify the effect of PI4KB on BC. The PRRS can effectively predict the prognosis and immunity of BC. Low PRRS was associated with a favorable prognosis, characterized by reduced tumor purity and enhanced immune cell infiltration. In addition, PRRS can help identify patients who are suitable for specific drug therapies. Finally, we found that PI4KB was highly expressed in BC. Knockdown of PI4KB expression significantly suppressed BC cell proliferation and migration. Our study establishes a robust framework for BC subtype classification and prognostic prediction, providing novel guidance for personalized therapeutic strategies. - Source: PubMed
Publication date: 2025/12/08
Dong ZiyiYang YanfangZhu MingyuLiu HuiGuo YaoyangZhang HaiyangJiang Zhansheng - Ciliogenesis requires an orchestrated interaction between the Golgi apparatus and centrioles via vesicle trafficking, yet this process is still poorly understood. Phosphatidylinositol 4-kinase β (PI4KB) is a conserved kinase that localizes to the Golgi for generating phosphatidylinositol 4-phosphate, an important lipid component related to cilium formation. Here, we demonstrate a previously uncharacterized mechanism of PI4KB in regulating Rab11a to enable proper ciliogenesis. PI4KB kinase activity maintains the normal vesicle density around the Golgi and Rab11a localization to centrioles in ciliogenesis. Inhibition of PI4KB activity leads to the reduced centriole localization but accumulation of Rab11a on the Golgi. We identified that the activation of Rab11a relies on PI4KB activity, while inactive Rab11a-guanosine diphosphate stably associates with Golgi and fails to undergo outward delivery. Autosomal-dominant nonsyndromic sensorineural hearing loss mutations in PI4KB abnormally intensify PI4KB interaction with Rab11a, leading to the aberrant subcellular Rab11a localization and defective ciliogenesis. Collectively, our study delineates a critical role for PI4KB in post-Golgi vesicle formation and ciliogenesis. - Source: PubMed
Publication date: 2025/12/05
Wang LiwenSun JiaSun ShuangHuang ZhenzhouLiu YingYan ShaodongSun ChunjiaoFeng QianFu WenxiangZhong TaoZhang JingjingZhou JunLiu Peiwei - Infection by positive-strand RNA viruses necessitates membrane expansion and elevated phospholipid biosynthesis, whereby fatty acids stored as triacylglycerols in lipid droplets (LDs) are mobilized to promote metabolic processes and membrane biogenesis. The replication organelles (ROs) of coronavirus associate with modified host endomembrane; however, the molecular mechanisms underlying the expansion and modification of these membranes remain poorly understood. Here, we show that viral protein orf3a collaborates with nsp3, nsp4, nsp6 to facilitate the formation of ROs in SARS-CoV-2. Importantly, orf3a targets LDs to ROs, establishing novel membrane contact sites and induces host cell microlipophagy, which supplies essential lipids for RO biogenesis. Subsequently, Following the formation of ROs, nsp3, with assistance from nsp12, indirectly recruits phosphatidylinositol 4-kinase beta (PI4KB) to ROs, to produce phosphatidylinositol 4-phosphate (PI4P). This action creates a PI4P-enriched microenvironment that enhances SARS-CoV-2 replication. Our findings elucidate the mechanism governing RO generation during SARS-CoV-2 infection and suggest that targeting microlipophagy pharmacologically may represent a promising strategy for the development of anti-coronaviruses therapies. - Source: PubMed
Publication date: 2025/11/21
Li ZhifeiHui XianfengZheng MiaomiaoHe ZhichengHuai WenjingDing BinbinJin MeilinQin YaliChen Mingzhou - Human rhinoviruses (RVs) are major pathogens responsible for respiratory tract infections, yet no approved antiviral therapy is available to combat these diseases. In this investigation, we designed and synthesized members of a group of 2-amido-3-aryl-4-methyl-thiazole derivatives and evaluated their inhibitory activities against phosphatidylinositol 4-kinase IIIβ (PI4KIIIβ), which is a host-directed target co-opted by a broad range of positive-sense RNA viruses, including RVs. In this series, 2e and 2f were found to exhibit strong activities against Group A and B RVs (EC = 0.06-0.843 μM), low cytotoxicities (CC ≥ 100 μM), high selectivity indices and additional submicromolar potencies against multiple coxsackieviruses. In addition, 2e and 2f retain high potencies (EC = 0.118 and 0.059 μM, respectively) against RV-C15. Both 2e and 2f selectively inhibit PI4KIIIβ more potently than they do PI4KIIIα, and they significantly reduce infectious virion production in plaque assays. Molecular dynamics simulations suggest that stable binding of these agents to PI4KIIIβ takes place through hydrogen bonding interactions with Lys549 and Ile595, and hydrophobic contacts with Ile547, Val598 and related residues. Results of in vitro liver microsomal assays indicate that 2e and 2f have moderate metabolic stability, and those arising from pharmacokinetic studies in rats demonstrate that they display favorable half-lives, oral bioavailabilities (52.4 % for 2e) and sustained plasma exposures. Collectively, these findings indicate that 2e (KR-27452) is a promising lead for the development of next-generation, broad-spectrum anti-RV agents targeting PI4KIIIβ. - Source: PubMed
Publication date: 2025/11/06
Ayele Sileshi AberaLee Hye LimSablon CélineReneesh AlbaSeo KyeongdeokJeong EunjuHwang DasomKim Mi JinKim Jae HakJung EunhyeLee Joo-YounJu YejinNeyts JohanThibaut Hendrik JanAhn SunjooLyoo HeyrhyoungShin Jin SooJung Young-Sik