Ask about this productRelated genes to: PFKM antibody
- Gene:
- PFKM NIH gene
- Name:
- phosphofructokinase, muscle
- Previous symbol:
- PFKX
- Synonyms:
- PFK-1, PPP1R122
- Chromosome:
- 12q13.11
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: PFKM antibody
Related articles to: PFKM antibody
- Cadmium (Cd) is an environmental nephrotoxicant that preferentially accumulates in the kidney and disrupts redox and energy metabolism. However, the protective effect of berberine (Ber) against Cd-induced nephrotoxicity remains insufficiently characterized. In the present study, male C57BL/6 mice were orally exposed to CdSO (30 mg/kg body weight/day) for 30 days in the absence or presence of berberine (25 or 100 mg/kg/day). Renal function, histopathology, oxidative stress parameters, LC-MS/MS-based metabolomic profiling, gene and protein expression, and in silico ligand-target interactions were evaluated. Cd exposure markedly increased serum CREA, renal index, renal LDH activity, and MDA content, decreased SOD and CAT activities, and induced pronounced renal histopathological lesions. Ber significantly attenuated these abnormalities in a dose-dependent manner. Metabolomic analysis revealed that Cd broadly suppressed pyruvate metabolism, tricarboxylic acid cycle intermediates, and NAD+/NADH homeostasis, whereas berberine restored the levels of pyruvate, acetyl-CoA, oxaloacetate, citrate, isocitrate, succinate, fumarate, malate, NAD+, and NADH. In parallel, berberine normalized the expression of metabolism-related genes including the downregulation of and the upregulation of , , , and , with the high-dose regimen showing the most evident recovery. Western blotting further verified the lower LDHA protein expression after berberine treatment. Molecular docking demonstrated favorable potential berberine-LDHA binding, and molecular dynamics simulation supported the stability of the ligand-protein complex. Collectively, these findings indicate that berberine ameliorates Cd-induced renal injury, an effect that correlates with attenuated oxidative stress, modulation of LDHA-associated glycolytic pathways, and restoration of mitochondrial TCA-cycle activity and redox balance, highlighting berberine as a promising candidate for the prevention of heavy metal-associated nephrotoxicity. - Source: PubMed
Publication date: 2026/06/26
Zeng ZikangQiao WeidongZhang YuanyuanTang Shusheng - Increasing evidence suggests that autoimmune responses are involved in the pathogenesis of age-related macular degeneration (AMD). This study aimed to characterize changes in serum autoantibody-captured retinal antigens in patients with different AMD subtypes, in order to gain further insight into the immunological mechanisms associated with AMD progression. - Source: PubMed
Publication date: 2026/07/08
Lin HaoWolfrum PeterKempf Lia FWolters DominikPerumal NatarajanKorb Christina AGrus Franz H - Insufficient lactation represents a critical global public health concern, profoundly impacting infant growth and development. Food-derived egg white peptides (EWPs) are believed to have lactation-promoting effects; however, the molecular mechanisms underlying these effects remain unclear. - Source: PubMed
Publication date: 2026/07/20
Zhai ChuangZan LitingYang RunjunLiu YuqingWu XuanFang XibiLiu Boqun - The kidney is a high-energy-consuming organ, and glucose is one of its principal fuel sources. It has been documented that disturbed renal glucose metabolism occurs in acute kidney injury (AKI), but whether these disturbances are consistent in different types of AKI, and how they change during the transition from AKI to chronic kidney disease (CKD), remain to be addressed. In this study, we used AKI models induced by cisplatin (20 mg/kg, 48 h), lipopolysaccharide (10 mg/kg, 48 h), and ischemia-reperfusion (48 h) to mimic distinct etiologies, and the catalytic enzymes involved in glucose transport, gluconeogenesis, and glycolysis were evaluated at both mRNA and protein levels by qPCR and immunofluorescence, respectively. In AKI, sodium-glucose cotransporter 2 (SGLT2) and fructose-1,6-bisphosphatase 1 (FBP1) protein levels were decreased, whereas the glycolytic enzymes hexokinase 2 (HK2), phosphofructokinase muscle type (PFKM), and pyruvate kinase M2 (PKM2) protein levels were increased. During the AKI-to-CKD transition, SGLT2 remained low; HK2, PFKM, PKM2, and FBP1 displayed a biphasic pattern (early rise, late fall) that varied with injury dose and time. These findings describe the expression changes of glucose metabolic enzymes during AKI and the AKI-to-CKD transition. Direct measurements of glycolytic activity were not performed; therefore, the relationship between these expression changes and actual metabolic function remains to be determined. This study provides a descriptive foundation for future investigations of glucose metabolism during the AKI-to-CKD transition. - Source: PubMed
Publication date: 2026/07/08
Yan ChaoyanZhu YutingLiu MingmingYang DieZha YanLong YanjunDong Rong - Most spinal muscular atrophy (SMA) patients develop severe scoliosis by late adolescence. Given that the paraspinal muscles-particularly the multifidus-are indispensable for maintaining spinal stability, their site-specific multi-omics characteristics in SMA remain insufficiently defined. Herein, integrated multi-omics sequencing was performed on bilateral multifidus samples from SMA patients and surgical controls. We identified 5219 differentially expressed genes, 1063 differentially expressed proteins and 370 differential metabolites between the control and SMA, showing significant enrichment in glucose and amino acid metabolism pathways, specifically key steps of glycolysis/gluconeogenesis. Key enzymes in the glycolytic process such as PFKM, ENO3 and PKM1 were markedly downregulated. Notably, a comparative analysis of the bilateral paraspinal muscles in SMA revealed asymmetrical metabolic signatures in carbohydrate and amino acid processing between the concave and convex sides. Key regulatory enzymes exhibited significant differential expression: PYGL, a central driver of starch and sucrose metabolism; creatine kinase, involved in arginine and proline metabolism; and PGAM2, a key mediator of glycine, serine, and threonine metabolism. These metabolic signatures indicate a complex metabolic reprogramming in the multifidus, where asymmetric disparities point to the influence of mechanical loading, while systemic dysregulation aligns with the effects of SMN depletion. - Source: PubMed
Wang ZhenZhao JunduoHuang Xu'anChen WeiyunShen Jianxiong