Ask about this productRelated genes to: OTOP3 antibody
- Gene:
- OTOP3 NIH gene
- Name:
- otopetrin 3
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 17q25.1
- Locus Type:
- gene with protein product
- Date approved:
- 2004-01-19
- Date modifiied:
- 2015-08-25
Related products to: OTOP3 antibody
Related articles to: OTOP3 antibody
- OTOP3, a proton channel located at 17q25.1, drives colorectal cancer growth by enhancing c-Myc stability and inhibiting ferroptosis through GPX4 regulation. Inhibition of OTOP3 suppresses CRC proliferation via c-Myc destabilization, ROS accumulation, and lipid peroxidation, while modulating metabolic shifts linked to the Warburg effect. These findings position OTOP3 as a novel therapeutic target for CRC by disrupting oncogenic signaling and ferroptosis resistance. - Source: PubMed
Publication date: 2025/06/30
Lee Yu JinHan Yeo JeongOh Je JoungKim Seung-YoungCho JaehoonJung Ji Hoon - The Otopetrin (Otop) proteins, comprising Otop1-3, are proton-gated proton channels with key biological functions. Otop1 acts as a receptor for sour and ammonium salt tastes in mammals, but its gating mechanisms and pharmacology remain poorly understood. Here, we report the functional characterization of three small molecule positive allosteric modulators of Otop1-MFaN, HIMOP, and B2FAMP-that enhance proton gating in a pH-dependent manner, potentiating Otop1 activity under weak acidic but not strong acidic conditions. HIMOP also uniquely enhances Otop1's alkali gating. These modulators preferentially target Otop1, sparing Otop2 and Otop3, and other ion channels. MFaN activates Otop1 while preserving its core biophysical and pharmacological properties by associating with key residues on the channel's S5-6 and S11-12 loops, including a crucial arginine (R554) essential for Zn and alkali activation. This study identifies important Otop1 modulators and structural elements underlying its gating, paving the way for further exploration of this ion channel. - Source: PubMed
Publication date: 2025/02/26
Kong XiangjinSun JieZhang HanhanYin YuanLiang XinyaoChen YanLuo GuoqingXia HuixinWang YoujunLiu ZhonghuaTang Cheng - Colorectal cancer is a common condition with an uncommon burden of disease, heterogeneity in manifestation, and no definitive treatment in the advanced stages. Renewed efforts to unravel the genetic drivers of colorectal cancer progression are paramount. Early-stage detection contributes to the success of cancer therapy and increases the likelihood of a favorable prognosis. Here, we have executed a comprehensive computational workflow aimed at uncovering the discrete stagewise genomic drivers of colorectal cancer progression. - Source: PubMed
Publication date: 2024/10/28
Palaniappan AshokMuthamilselvan SangeethaSarathi Arjun - Eggshell is one of the most important indicators of egg quality, and due to low shell strength, pimple eggs (PE) are more susceptible to breakage, thus causing huge economic losses to the egg industry. At the current time, the molecular mechanisms that regulate the formation of pimple eggs are poorly understood. In this study, uterine tissues of PE-laying hens (n = 8) and normal egg (NE) -laying hens (n = 8) were analyzed by whole transcriptome sequencing, and a total of 619 differentially expressed mRNAs (DE mRNAs), 122 differentially expressed lncRNAs (DE lncRNAs) and 21 differentially expressed miRNAs (DE miRNAs) were obtained. Based on the targeting relationship among DE mRNAs, DE lncRNAs and DE miRNAs, we constructed a competitive endogenous RNA (ceRNA) network including 12 DE miRNAs, 19 DE lncRNAs, and 128 DE mRNAs. Considering the large amount of information contained in the network, we constructed a smaller ceRNA network to better understand the complex mechanisms of pimple egg formation. The smaller ceRNA network network contains 7 DE lncRNAs (LOC107056551, LOC121109367, LOC121108909, LOC121108862, LOC112530033, LOC121113165, LOC107054145), 5 DE miRNAs (gga-miR-6568-3p, gga-miR-31-5p, gga-miR-18b-3p, gga-miR-1759-3p, gga-miR-12240-3p) and 7 DE mRNAs (CABP1, DNAJC5, HCN3, HPCA, IBSP, KCNT1, OTOP3), and these differentially expressed genes may play key regulatory roles in the formation of pimpled eggs in hens. This study provides the overall expression profiles of mRNAs, lncRNAs and miRNAs in the uterine tissues of hens, which provides a theoretical basis for further research on the molecular mechanisms of pimpled egg formation, and has potential applications in improving eggshell quality. - Source: PubMed
Publication date: 2024/04/07
Li WenqiangCao ZhiXu FeiZhang XuguangSun YifeiXie ZhongbiaoNing ChaoZhang QinWang DanTang Hui - Butyrate contributes epigenetically to the changes in cellular function and tissue development of the rumen in ruminant animals, which might be achieved by its genetic or epigenetic regulation of gene expression. To explore the role of butyrate on bovine rumen epithelial function and development, this study characterized genome-wide H3K27ac modification changes and super-enhancer profiles in rumen epithelial primary cells (REPC) induced with butyrate by ChIP-seq, and analyzed its effects on gene expression and functional pathways by integrating RNA-seq data. The results showed that genome-wide acetylation modification was observed in the REPC with 94,675 and 48,688 peaks in the butyrate treatment and control group, respectively. A total of 9750 and 5020 genes with increased modification (H3K27ac-gain) and decreased modification (H3K27ac-loss) were detected in the treatment group. The super-enhancer associated genes in the butyrate-induction group were involved in the AMPK signaling pathway, MAPK signaling pathway, and ECM-receptor interaction. Finally, the up-regulated genes (, , , , ) with H3K27ac gain modification by butyrate were involved in cholesterol metabolism, lysosome, cell adhesion molecules, and the PI3K-Akt signaling pathway. Butyrate treatment has the role of genome-wide H3K27ac acetylation on bovine REPC, and affects the changes in gene expression. The effect of butyrate on gene expression correlates with the acetylation of the H3K27ac level. Identifying genome-wide acetylation modifications and expressed genes of butyrate in bovine REPC cells will expand the understanding of the biological role of butyrate and its acetylation. - Source: PubMed
Publication date: 2023/07/16
Kang XiaolongLi ChenglongLiu ShuliBaldwin Ransom LLiu George ELi Cong-Jun