Ask about this productRelated genes to: HSD3B7 antibody
- Gene:
- HSD3B7 NIH gene
- Name:
- hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
- Previous symbol:
- -
- Synonyms:
- C(27)-3BETA-HSD, SDR11E3
- Chromosome:
- 16p11.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-01-13
- Date modifiied:
- 2016-07-18
Related products to: HSD3B7 antibody
Related articles to: HSD3B7 antibody
- Primary bile acid synthesis defects (BASD) are autosomal recessive disorders causing cholestatic liver disease and fat-soluble vitamin malabsorption. Despite being treatable with cholic acid (CA), BASD remain underdiagnosed, particularly in resource-limited settings. No data have been published from Tunisia. - Source: PubMed
Publication date: 2026/09/09
Zribi MounaKhatrouch SafaBoudabous HelaBen Messaoud SanaHierro Llanillo LoretoLaplanche SophieSpraul AnneBen Chehida AmelAbdelmoula Mohamed Slim - Tuberculosis (TB), caused predominantly by Mycobacterium tuberculosis (Mtb), remains a major global health challenge despite the availability of antimicrobial chemotherapy. Drug-resistant TB, latent infection, immunopathology and metabolic comorbidities such as diabetes continue to undermine treatment efficacy, highlighting the urgent need for host-directed therapeutic (HDT) strategies that complement antibacterial regimens. G protein-coupled receptors (GPCRs) are highly tractable drug targets that integrate immune and metabolic signals. Among them, GPR183, also known as Epstein‒Barr virus-induced gene 2 (EBI2), has emerged as an oxysterol-sensing receptor with growing relevance to TB pathogenesis. - Source: PubMed
Wang JunfeiZhou YingnanJi XunmingZhao HaipingZhan Lingjun - Steroid hormones regulate ovarian development, but hsd3b7 function in fish remains unclear. In this study, we cloned full-length hsd3b7 cDNA from Qihe gibel carp (Carassius gibelio var. Qihe) and generated a polyclonal antibody. Real-time PCR, Western blotting, and immunohistochemistry revealed widespread hsd3b7 expression in brain, heart, spleen, muscle and ovary. During ovarian development and maturation, hsd3b7 mRNA expression peaked at 150 days after hatching (dah) and remained at a relatively high level until 720 dah. Immunohistochemical analysis further revealed that Hsd3b7 protein was localized in both gonadal germ cells and somatic cells, suggesting its involvement in ovarian development and maturation. To further investigate its function, hsd3b7 was knocked down for 30 days by ovarian injection of double-stranded RNA (dsRNA) during the previtellogenic stage. Silencing of hsd3b7 markedly inhibited ovarian development, with most oocytes remaining at phase II. In addition, the expression levels of the germ cell marker genes vasa and piwil, as well as the cell proliferation-related genes pcna and ki-67, were significantly reduced. Protein interaction analysis suggested that Hsd3b7 is associated with several steroidogenic enzymes, including Cyp19a1a, Cyp11c1, Hsd17b3, and Hsd17b7. Moreover, hsd3b7 knockdown altered the expression of genes involved in steroidogenesis and lipid accumulation, and significantly affected serum E2 and total cholesterol levels. Collectively, these findings indicate hsd3b7 regulates ovarian development and maturation via steroid hormone synthesis and lipid metabolism, providing insights for addressing precocious maturation in Qihe gibel carp. - Source: PubMed
Publication date: 2026/08/13
Li YongjingJin YujiaoLi YuqiXu YufengLi XuejunWu Limin - 3β-Hydroxysteroid dehydrogenases (3βHSDs) are key enzymes in steroid metabolism, catalyzing C3 oxidation-reduction and Δ5→Δ4 isomerization reactions that govern metabolic flux across multiple steroidogenic pathways. However, the functional diversity of 3βHSDs involved in bufadienolide metabolism in amphibians remains poorly explored. Here, we systematically characterized the 3βHSD gene family in the Asian toad (Bufo bufo gargarizans) using integrated transcriptomic, biochemical, and metabolomic analyses. Seven Bg-3βHSD genes were identified from multi-tissue transcriptomes generated under control and Pb2+ exposure conditions, and six were heterologously expressed for functional evaluation. In vitro assays revealed pronounced functional divergence among Bg-3βHSD isoforms. Bg-3βHSD1 primarily catalyzed bidirectional C3 redox reactions of C21 steroids and bile acid-related substrates, consistent with canonical steroidogenic roles. In contrast, Bg-3βHSD2 enzyme exhibited broad substrate specificity and high catalytic efficiency toward hormones, bile acids, and bufadienolides. In addition to canonical C3 redox reactions and Δ5→Δ4 isomerization, Bg-3βHSD2 also displayed additional oxidation activity at the C17 position for several steroid substrates. A third homolog, Bg-HSD3B7 (GenBank accession no. XM 044303756.1), selectively converted 7α-hydroxylated sterols, suggesting a potential role in classical bile acid metabolism. Integration of tissue-specific expression profiles with bufadienolide distribution patterns suggests that Bg-3βHSD2 may contribute to connecting classical steroid metabolism with bufadienolide biosynthesis in adrenal tissue. Together, the present study identifies Bg-3βHSD2 as an efficient and versatile steroid-transforming enzyme, expands our knowledge of functional diversity within the amphibian 3βHSD family, and provides insights into the enzymatic basis of steroid and bufadienolide metabolism in B. bufo gargarizans. - Source: PubMed
Meng QianHu YaotingChen WenjieXiao ChuanguangYang LiWang RufengZhang BeibeiZhao ShujuanWang Zhengtao - : Bile acid synthesis disorders (BASDs) represent a distinct category of progressive familiar cholestatic liver disease. A novel targeted mass spectrometry assay was developed for the accurate measurement of the major urinary atypical bile acids and bile alcohols that are biomarkers for HSD3B7, AKR1D1, CYP7B1 and CYP27A1 deficiencies, the four most common BASDs. Stable-isotope dilution UPLC tandem mass spectrometry was used for the simultaneous quantification of 12 key atypical bile acid biomarkers in urine from patients with BASD. Typical concentration ranges for these metabolites were established from urine samples from patients with biochemically and/or genetically confirmed BASD and compared with non-cholestatic and cholestatic controls. The separation of major 3β-hydroxy-Δ-bile acid sulfates, taurine- and glycine-conjugated 3-oxo-Δ-bile acids, and bile alcohol glucuronides was achieved in a 20 min chromatographic run with intra- and inter-batch imprecisions of <15% for all metabolites. The mean ± SEM urinary concentration of total 3β-sulfated-Δ-cholenoic acids in patients with HSD3B7 deficiency was 704 ± 204 µmol/L (n = 22), approximately 2000-fold higher than in cholestastic patients (n = 168) or non-cholestatic controls (n = 127). Similarly, the concentration of 5β-cholestane-3α,7α,12α,24,25-pentol-glucuronide, the major bile alcohol, in patients with CYP27A1 deficiency was 95 ± 17 µmol/L (n = 12). For CYP7B1 deficiency, two confirmed cases showed elevated levels (average, 7.5 µmol/L) of the glycine conjugate of 3β-sulfooxy-Δ-bile acid. In AKR1D1 deficiency, total 3-oxo-Δ-bile acids in urine were elevated (81 ± 16 µmol/L, n = 48), but concentrations showed overlap with cholestatic and non-cholestatic controls. : A novel quantitative tandem mass spectrometry assay is described for the measurement of the major atypical metabolites and biomarkers in urine applicable to the accurate monitoring of treatment responses, and for the first time typical concentration ranges are established for each of these BASDs. - Source: PubMed
Publication date: 2026/06/23
Setchell Kenneth D RZhao XuehengReed StaceyZhang Wujuan