Ask about this productRelated genes to: HSD11B1 antibody
- Gene:
- HSD11B1 NIH gene
- Name:
- hydroxysteroid 11-beta dehydrogenase 1
- Previous symbol:
- HSD11B, HSD11
- Synonyms:
- SDR26C1
- Chromosome:
- 1q32.2
- Locus Type:
- gene with protein product
- Date approved:
- 1991-11-14
- Date modifiied:
- 2016-10-05
Related products to: HSD11B1 antibody
Related articles to: HSD11B1 antibody
- Enterobacterial infections being severe, with a high mortality rate, particularly affecting ICU patients, and 80% newborns. These infections have multifactorial multi-drug resistance (MDR) mechanisms, which frequently causes current antibiotic therapies to fail, mortality rate remains high, and creating an urgent need for alternative multi-targeted therapies, such as plant-derived compounds to restore clinical effectiveness. This study aims to discover novel anti enteric compounds in Hydrocotyle javanica Thunb. (H. javanica) belongs to Apiaceae family and understanding their interaction mechanism with enterobacterial infection targeted genes, using network pharmacology with in-silico docking and molecular dynamics simulation approaches. Four bioactive compounds as tetracosanoic acid, alpha-Amyrenyl acetate, stigmasterol glucoside, stigmasterol were identified as potential therapeutic agents. Most of the compounds exhibited favourable pharmacokinetic properties, complying with Lipinski's rule, with high bioavailability score 0.85 and non-toxic profiles. In-silico antibacterial prediction indicated both bacterial and bacteriostatic activities. A total of 53 common targets were identified with network analysis revealing key hub genes including HSD11B1, PTGS2,FDFT1,CYPHA1, and AKR1C2. Functional enrichment analysis showed significant involvement in immune and inflammatory pathways, particularly calcium signaling, MAPK signaling and reactive oxygen species related pathways. The docking result showed highest binding affinity, with stigmasterol showing highest score (-10.7 kcal/mol). A 100 ns molecular dynamics simulation further confirmed the stability of the stigmasterol-protein complex, with stable RMSD values 1-2 Å, low RMSF fluctuations and consistent hydrogen bonding, indicating sustained structural integrity. The present study highlights that phytocompounds from Hydrocotyle javanica exhibit significant binding affinity toward key enterobacterial targets, along with favorable ADME and toxicity profiles. These findings suggest their potential as promising lead molecules for anti-enterobacterial drug development, warranting further experimental validation. - Source: PubMed
Publication date: 2026/08/20
Paul DebasmitaGhosh MeghaMandal Manab - Taraxasterol (TAR) exerts therapeutic effects on various liver diseases via its inherent hepatoprotective and anti-inflammatory properties. However, the mechanism by which TAR treats hepatic fibrosis remains unclear. This study aims to identify the potential targets and specific molecular mechanisms underlying the therapeutic effects of TAR on hepatic fibrosis. DDC- and CCl -induced mouse models of hepatic fibrosis are established. The human hepatic stellate cell (HSC) line LX-2 and primary mouse HSCs are used for experiments. Transcriptomics, network pharmacology, single-cell transcriptomics, and molecular docking are employed to identify potential therapeutic targets of TAR for hepatic fibrosis. Functional validation is performed via HSD11B1 overexpression and knockdown experiments, and the regulatory role of the ERK MAPK pathway is verified using the specific inhibitor U0126. Histological staining results show that TAR significantly alleviates DDC- and CCl -induced hepatic fibrosis in mice and reduces associated liver injury. assays reveal that TAR effectively reverses TGF-β-induced activation of LX-2 cells and primary mouse HSCs. Multi-omics and docking analyses identify HSD11B1 as a direct target of TAR, whose downregulation in activated HSCs is restored by TAR treatment. Functional experiments demonstrate that overexpression of HSD11B1 attenuates TGF-β-induced HSC activation, while knockdown abolishes the therapeutic effects of TAR. Subsequent transcriptomic analysis confirms that HSD11B1 suppresses the ERK MAPK pathway, and knockdown of compromises the therapeutic efficacy of the ERK MAPK inhibitor U0126. In summary, TAR alleviates liver fibrosis by inhibiting HSC activation through the HSD11B1-ERK MAPK axis. - Source: PubMed
Publication date: 2026/08/03
Wang PengZhou HaoxiongDing YumingChen YanLiu HuilingJiang JieWu Bin - Non-small cell lung cancer (NSCLC) is the predominant subtype of lung malignancy. Accumulating epidemiological evidence demonstrates that environmental lead (Pb) exposure as a critical driver of its initiation and progression. - Source: PubMed
Publication date: 2026/07/30
Xu YinlanDong JieLiu HejunZheng JinmeiGuo HaonanChen YueWang JialinWu Weidong - While magnesium isoglycyrrhizinate (MgIG) is a clinically approved therapy for alcohol-associated liver disease (ALD), its precise molecular targets and mechanisms remain uncharacterized. This study aimed to define MgIG's hepatoprotective actions in chronic-binge ALD mouse models and ethanol/palmitic acid-exposed AML-12 hepatocytes. Through an integrated strategy encompassing RNA sequencing, molecular docking, and microscale thermophoresis, we discovered that MgIG directly binds to hydroxysteroid 11-beta dehydrogenase 1 (HSD11B1) at residue 187, a finding corroborated by molecular dynamics simulations. In vivo, MgIG markedly attenuated alcohol-induced liver injury, evidenced by ameliorated histological damage, reduced hepatic steatosis, and normalized liver-to-body weight ratios. In vitro, it effectively reduced lipid accumulation, inflammation, and apoptosis. Mechanistically, RNA sequencing identified isopentenyl diphosphate delta isomerase 1 (IDI1) as a key downstream effector. Hepatocyte-specific genetic manipulations confirmed that MgIG modulates the SREBP2-IDI1 axis, thereby suppressing lipogenesis, inflammatory responses, and apoptotic pathways. We reveal HSD11B1 as a novel direct molecular target of MgIG and elucidate its therapeutic mechanism through the HSD11B1-SREBP2-IDI1 signaling axis, which profoundly impacts ALD pathogenesis. These findings not only validate MgIG's clinical utility but also highlight a promising new therapeutic target for ALD. - Source: PubMed
Publication date: 2026/07/28
Xiao LuLi LuWu ShashaChe ZhaoyiDu YuyangZheng JingyiYan JingsongWang HaoZhang HongLi YanXiao Jia - Lactation and mammary involution and remodeling are complex biological processes that require the coordinate expression of thousands of genes. Differential expression analyses comparing lactating and dry goats have revealed extensive changes in the expression of protein-coding and non-coding RNAs in the mammary gland. Here, we hypothesize that lactation and mammary involution/remodeling may also involve changes in the abundance of transcripts differing in exon composition and functional properties. To test this hypothesis, we analyzed the mammary transcriptomes of 5 lactating and 4 dry goats by using a hybrid approach based on the integration of data from short-read Illumina and long-read Nanopore sequencing. After data filtering, we detected 21,598 transcripts derived from 12,300 genes (≈1.7 transcripts per locus) in the goat mammary gland. Among them, we found 14,092 annotated isoforms, 6,291 novel isoforms, and 1,215 novel loci. Around 39.3% of expressed genes generated multiple transcript variants. Overall, the goat mammary transcriptome showed exon skipping as the most frequent splicing event, followed by alternative use of initial exons, intron retention, and variations in 3' and 5' splicing sites. We also observed that a limited number of isoforms accounted for a very substantial fraction of the total expression output of the lactating mammary gland, with the top 10 and top 50 genes representing approximately 66% and 82% of total expression, respectively. This transcriptomic specialization is driven primarily by genes encoding caseins CSN1S1, CSN2, and CSN3, and major whey proteins such as progestagen associated endometrial protein (PAEP), α-lactalbumin (LALBA), and lactophorin (GLYCAM1), which are essential milk nutrients. Finally, differential transcript usage (DTU) analysis comparing lactating and dry goats revealed 443 isoform switches affecting 355 unique genes and 563 transcripts. Besides, 266 and 297 transcripts were upregulated and downregulated in lactating goats, respectively, and 413 DTU, affecting 248 genes, were predicted to have functional consequences. Among these functional consequences, the most important ones were protein domain gain, non-reference domain isoform gain, nonsense-mediated insensitivity and coding transcripts. Several of the genes showing DTU have important roles in lactation, being of particular relevance those encoding epidermal growth factor receptor (EGFR), glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), hydroxysteroid 11-β dehydrogenase 1 (HSD11B1), insulin receptor substrate 1 (IRS1), nuclear receptor subfamily 3 group C member 1 (NR3C1), and phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1). Moreover, we also detected DTU for several genes integrated in the mitogen-activated protein kinase and Rho GTPase pathways. In summary, we provide a comprehensive catalog of RNA isoforms expressed in the goat mammary gland and demonstrate that mammary transcript splicing patterns differ substantially between lactating and dry goats. - Source: PubMed
Publication date: 2026/07/27
Cardoso T FWang MNoce ALuigi-Sierra MMartínez ADelgado-Bermejo J VSalama A A KSuch F XJordana JAmills M