Ask about this productRelated genes to: HAS3 antibody
- Gene:
- HAS3 NIH gene
- Name:
- hyaluronan synthase 3
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 16q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 1996-10-18
- Date modifiied:
- 2015-09-11
Related products to: HAS3 antibody
Related articles to: HAS3 antibody
- Hyaluronan (HA) is a major glycosaminoglycan of the extracellular matrix that regulates cell migration, signaling, and tissue homeostasis. Its turnover is controlled by coordinated synthesis by HA synthases and degradation by hyaluronidases. Among these hyaluronidases, TMEM2, the only known transmembrane hyaluronidase, plays a unique role in HA degradation at the cell surface; however, the cellular conditions that support its activity remain incompletely understood. To address this, we developed a cell-based HA turnover assay to examine TMEM2-mediated degradation of endogenously synthesized HA, rather than exogenously added, fluorescently labeled HA used in previous studies. Using this system, we show that TMEM2 readily degrades high-molecular weight HA synthesized by co-expressed HAS3. This degradation occurs only when TMEM2 and HAS3 are co-expressed in the same cells (), whereas co-culture of TMEM2-expressing cells with HAS3-expressing cells supports little or no degradation. Interestingly, HA-binding cell surface receptors CD44 and its homolog LYVE-1 promote efficient TMEM2-mediated HA degradation even under conditions, whereas other HA-binding proteins, including TSG-6, layilin, TLR2, RHAMM, and ICAM-1, do not. These findings suggest a spatially regulated mechanism of TMEM2 activity in which capture of HA at the cell surface, mediated by CD44 or LYVE-1, contributes to efficient HA degradation by TMEM2. - Source: PubMed
Publication date: 2026/08/12
Tobisawa YukiYano FumiakiraTomioka-Inagawa RisaIrie FumitoshiKoie TakuyaYamaguchi Yu - The growing demand for safe, naturally derived active ingredients in cosmetic and dermatological formulations has accelerated interest in green nanomaterials with multifunctional skin-protective properties. This study presents the microwave-assisted synthesis and comparative evaluation of carbon dots (CDs) derived from hyaluronic acid (HA) and eastern prickly pear cactus (Opuntia humifusa (O. humifusa)) for their moisturizing and antioxidant properties. Both CDs exhibited strong UV absorption at 264 nm and characteristic blue photoluminescence around 450 nm, indicating their potential as UV-protective cosmetic nanomaterials. In antioxidant assays, O. humifusa-CD exhibited significantly higher radical scavenging activity (p < 0.05), with an estimated ABTS IC₅₀ value of 585 µg/mL compared with 710 µg/mL for HA-CD. Similarly, both CDs showed significant intracellular ROS scavenging activity in HO-stimulated HaCaT cells, achieving close to 88% and 77% ROS inhibition at 500 µg/mL after 24 h and 48 h, respectively. Moisturizing activity was evaluated at the molecular level through western blot analysis. HA-CDs significantly upregulated the expression of AQP3, HAS-2, and HAS-3 by approximately 1.77-, 2.05-, and 2.24-fold, respectively. On the other hand, O. humifusa-CDs exhibited significantly greater anti-inflammatory activity (p < 0.05), reducing NO production by approximately 74% in LPS-stimulated RAW 264.7 macrophages. These findings highlight the multifunctional potential of HA-CDs and O. humifusa-CDs as natural and biocompatible materials, making them suitable for incorporation into advanced topical formulations for skincare. Although the present findings demonstrate the promising antioxidant, moisturizing, and anti-inflammatory potential of the synthesized CDs in vitro, further in vivo studies and clinical evaluations are necessary to confirm their safety and effectiveness for practical cosmetic applications. - Source: PubMed
Publication date: 2026/07/20
Youn Jeong-YunJaison AugustineChoi Hyeon SeokNgoc Le Thi NhuMoon Ju-YoungJeong SugyeongLee Young-Chul - Hyaluronan (HA) is a key component of the pericellular and extracellular matrix and is synthesized by both non-hematopoietic and myeloid cells. In this study, we demonstrate that adaptive immune T cells not only respond to exogenously added high-molecular-weight HA by reducing proliferation but also generate an endogenous HA-rich glycocalyx in a stage-specific manner, as shown by fluorescence assisted carbohydrate electrophoresis and imaging studies. HA in the glycocalyx of naive CD4 T cells, localized with the HA-binding proteoglycan, versican (VCAN). During differentiation into effector subsets, pericellular HA/VCAN is removed, with HA detected intracellularly, often co-localized with CD44. Hyaluronan synthase 3 (HAS3) is the predominant enzyme in naive CD4 T cells, as HAS3-deficient T cells display minimal surface HA and exhibit extensive proliferation. We discuss the significance of the metabolic turnover of the HA/VCAN glycocalyx in T cells as a mechanism to regulate activation and proliferation. - Source: PubMed
Publication date: 2026/06/15
Adhikari Laxmi PrasadContreras JessicaPatterson Kristen MMoran Meghan MHowe Erin NPlaas Anna H KReynolds Joseph M - Coarse particulate matter (PM) deposits at the vocal fold (VF) mucosa, yet upper airway responses remain poorly characterized. Existing VF models use monocultures that lack the stratified epithelium, lamina propria, and physiological perfusion. We developed a chip-based co-culture model of human VF mucosa and applied it to acute PM exposure. - Source: PubMed
Publication date: 2026/06/02
Coburn Patrick TMunipalle MeghanaLiu YinLungova VlastaThapa SamjhanaMartignetti LisaLiu XinyuMaussion GillesChen Carol X-QDurcan Thomas MThibeault Susan LLi-Jessen Nicole Y K - Sex differences influence cancer incidence, treatment response, and susceptibility to cardiovascular toxicity. Males exhibit higher rates and poorer outcomes in most non-sex-specific cancers, while females more frequently experience treatment-related adverse events, including cancer therapy-related cardiac dysfunction. Biological factors such as hormonal status, genetic polymorphisms, immune responses, and pharmacokinetics contribute to these disparities. In cardio-oncology, women-particularly premenopausal or with specific genotypes-may be at increased risk for cardiotoxicity after treatment with anthracyclines, immune checkpoint inhibitors or radiotherapy. Clonal hematopoiesis and certain germline genetic variants such as single nucleotide polymorphisms (e.g., RARG rs2229774, HAS3 rs2232228) are emerging as potential sex-informed biomarkers for predicting cardiotoxicity risk. Despite growing evidence, sex remains insufficiently integrated into clinical trials and guideline development in cardio-oncology. This review highlights the importance of sex-specific surveillance, prevention, and multi-omic risk stratification to advance precision cardio-oncology and support better outcomes for patients across the cancer care continuum. - Source: PubMed
Publication date: 2026/05/22
Keramida KalliopiAznar Marianne CBergler-Klein JuttaBoriani GiuseppeCardinale DanielaDent SusanDrakaki AlexandraFuster Jose JMamas Mamas AOkwuosa TochiScarfo LydiaVan Der Meer PeterYang Eric HLopez-Fernandez Teresa