Ask about this productRelated genes to: NT5E protein
- Gene:
- NT5E NIH gene
- Name:
- 5'-nucleotidase ecto
- Previous symbol:
- NT5
- Synonyms:
- CD73, eN, eNT, CALJA
- Chromosome:
- 6q14.3
- Locus Type:
- gene with protein product
- Date approved:
- 1989-02-23
- Date modifiied:
- 2016-10-05
Related products to: NT5E protein
Related articles to: NT5E protein
- Urothelial bladder cancer (BCa) is marked by high recurrence and mortality, and the efficacy of PD-1/PD-L1 immunotherapy remains limited because of immune evasion. The adenosinergic pathway (AP), mediated by ectonucleotidases CD39 and CD73, is a key immunosuppressive mechanism, but its role in BCa remains unclear. We conducted an integrated immunophenotypic analysis of peripheral blood (PB) and the tumor microenvironment (TME) from 39 patients with BCa and 14 healthy controls using multicolor flow cytometry and immunohistochemistry. High-risk (HR) patients exhibited systemic immunosuppression, characterized by an elevated neutrophil-to-lymphocyte ratio and increased circulating regulatory T cells (Tregs), along with reduced cytotoxic γδ T cells and diminished Th1/Tc1 functional subtypes. In the TME, we observed reduced CD8 T cell infiltration accompanied by increased Tregs, and phenotypes characterized by poor immune infiltration in the tumor core predominated across the cohort. In both PB and the TME, CD39 and CD73 expression on T cells strongly correlated with an immunosuppressive environment, marked by increased M2-like macrophages and decreased effector T cells. Circulating double-positive T cells (CD4CD39CD73 and CD8CD39CD73) mirrored the intratumoral T-cell composition, suggesting their potential as non-invasive biomarkers. Elevated frequencies of circulating single-positive CD8CD39 and CD8CD73 T-cells were significantly associated with higher pathological grade and distinguished high-grade tumors with moderate accuracy (AUC > 0.70). This study demonstrates that BCa is characterized by extensive AP-linked immunosuppression, and that specific ectonucleotidase-expressing circulating T-cell subsets may serve as non-invasive biomarkers for assessing tumor infiltration and grade. - Source: PubMed
Publication date: 2026/04/22
Furriel FredericoLaranjeira PaulaPereira MargaridaSilva SandraSilva IsabelFontinha GuilhermeSousa VítorGomes CéliaParada BelmiroPaiva Artur - Pulmonary hypertension (PH) is a severe progressive disease characterised by elevated pulmonary vascular resistance and right ventricular hypertrophy. Increasing evidence has highlighted the vital role of nicotinamide adenine dinucleotide (NAD) metabolism in cardiovascular disease. However, the role of NAD metabolism-related genes (NMRGs) in PH remains unclear. In this study, we aimed to identify novel NMRGs as biomarkers in PH. - Source: PubMed
Publication date: 2026/04/07
Chu YananYang JinxiuYe TianxinYu FangcongWang QianZhuo Jingming - Pancreatic Cancer (PC) is a highly aggressive malignancy with a dismal prognosis, primarily due to late-stage diagnosis and limited therapeutic options. This study aimed to identify potential biomarkers involved in PC progression and immune microenvironment modulation. - Source: PubMed
Publication date: 2026/04/18
Dong ChangjunGuan JingDong LinhuanYu YunlinZhang XiangweiLi ZhengZhang XianlinMo Chunlin - A 52-year-old man presented with sarcomatoid diffuse pleural mesothelioma that had relapsed at an isolated site after a complete response to dual-immune checkpoint inhibition (ICI). Targeted sequencing exhibited amplification of chromosome 9p24, encompassing JAK2, PD-L1, PD-L2, and PTPRD in the relapsed (post-ICI) tumor, compared with baseline (pre-ICI). On multiplex immunofluorescence, tumor-associated macrophages (TAMs) and CD8 cytotoxic T lymphocytes (CTLs) made up most of the cells in baseline and relapsed tumor (59% and 47%, respectively). Baseline tumor cells expressed genes linked to extracellular matrix remodeling and epithelial-mesenchymal transition, intermixed with M2-like TAMs and tissue-resident, effector-like CTLs. Relapsed tumor cells shifted to a growth factor-driven phenotype (NT5E, NOD1, GATA2, FN1, PDCD1LG2) that is known to cause functional impairment of CTLs, which then transitioned to an exhausted state (FCRL3, CST7, GPR171, TRAT1, LAG3); exhausted CD8 and CD4 T cells are seen in the peripheral blood at relapse. TAMs were enriched in antigen-presentation (CD80, CD86, CXCL10), extracellular matrix-degradation (MMP9, CTSL), and CTL-suppression (ARG1, PLA2G7) pathways. Our analyses revealed that regional immunosuppression mediated by adaptive reprogramming of tumor-cell and immune-cell (TAMs, CTLs)-intrinsic changes-rather than by immune evasion or stromal exclusion-served as a mechanism of acquired resistance to dual-ICI therapy. - Source: PubMed
Publication date: 2026/04/15
Ollila HelyKulkarni PrateekKim HyojinAnkola PratitiChintala Navin KThomas CarlosSauter Jennifer LOffin MichaelAdusumilli Prasad S - Nicotinamide adenine dinucleotide (NAD) is essential for maintaining homeostasis in all types of cells, including endothelium, and depletion of its pool can impair bioenergetics and stress response, contributing to cardiovascular disorders. Nicotinamide riboside (NR) effectively restores the intracellular NAD pool, supporting endothelial integrity, but the molecular mechanisms remain incompletely elucidated, particularly regarding extracellular adenine nucleotide catabolism, purinergic signaling, and their effects on immune cell adhesion. In this study, we aimed to investigate the effects of NR on intracellular nucleotides, extracellular adenine nucleotide catabolism, and adhesive properties in the cultured murine (H5V) and human (HMEC-1) microvascular endothelial cell line. We demonstrated that NR treatment significantly increased intracellular NAD concentrations without changes in the energy status of endothelial cells. We also showed that NR treatment accelerated extracellular hydrolysis of ATP and AMP and decreased the rate of adenosine deamination in endothelial cells. Moreover, we observed CD73 activity and adenosine-related reduced adhesion of T-lymphocytes, monocytes and platelets to the NR-treated endothelial monolayer. Our findings highlight a previously unrecognized role of NR in maintaining endothelial homeostasis, showing that NR is not only a potent intracellular NAD booster in endothelial cells but also affects extracellular nucleotide metabolism in a way that promotes cytoprotective adenosine formation. - Source: PubMed
Publication date: 2026/04/03
Harasim-Krawcewicz GabrielaMierzejewska PaulinaKawecka AdaFrańczak Marika AKról OliwiaPresler MałgorzataJędrzejewska AgataKutryb-Zając BarbaraSmoleński Ryszard TSłomińska Ewa