Ask about this productRelated genes to: CNDP2 antibody
- Gene:
- CNDP2 NIH gene
- Name:
- carnosine dipeptidase 2
- Previous symbol:
- PEPA
- Synonyms:
- FLJ10830, CN2, HsT2298, CPGL
- Chromosome:
- 18q22.3
- Locus Type:
- gene with protein product
- Date approved:
- 2004-04-14
- Date modifiied:
- 2017-03-23
Related products to: CNDP2 antibody
Related articles to: CNDP2 antibody
- Pulmonary large cell neuroendocrine carcinoma (LCNEC) is a rare and aggressive lung cancer with limited therapeutic options and poorly defined metabolic features. To establish a comprehensive molecular overview, we generated the first metabolomics and lipidomics atlas of LCNEC using paired tumor and adjacent non-tumor lung tissues from 34 patients. Untargeted multiplatform liquid chromatography-mass spectrometry profiled 1052 metabolites, revealing extensive remodeling of amino acid, nucleotide, and lipid metabolism. Tumor tissues showed pronounced accumulation of both D- and L-2-hydroxyglutaric acid, indicating altered α-ketoglutarate metabolism independent of IDH1/2 mutations. Newly identified N-lactoyl-amino acids, formed via CNDP2-mediated condensation of lactate and amino acids, were uniformly elevated, suggesting enhanced lactoyl conjugation under elevated lactate levels. Lipidomic profiling revealed widespread reprogramming, including increased phosphatidylcholines, ether-linked phospholipids, polyunsaturated bis(monoacylglycero)phosphates, long-chain triacylglycerols, cholesteryl esters, and acylcarnitines, indicative of lysosomal remodeling and altered mitochondrial fatty acid transport. In addition, the nicotine metabolite cotinine was quantified as an objective biomarker of smoking exposure, revealing discrepancies between measured cotinine levels and self-reported smoking status in several patients. This highlights the value of metabolomics for independently verifying clinical information. Collectively, these data define a hybrid metabolic phenotype bridging features of small and non-small cell lung cancer while revealing unique metabolic signatures of LCNEC. The resulting atlas provides a foundational resource for biomarker discovery and the development of metabolism-based therapeutic strategies in this understudied lung cancer subtype. - Source: PubMed
Publication date: 2026/04/07
Tietzova IlonaHricko JiriRancourt Rebecca CSchneider Marc AAllgäuer MichaelCajka Tomas - Duck meat is highly appreciated for its unique flavor and rich nutritional value, and metabolites have become important phenotypic indicators of meat quality. The Sansui duck, a celebrated local breed from Guizhou, is known for its tender, savory meat, yet its metabolomic composition and underlying genetic basis remain unexplored. In our study, non-targeted LC-MS/MS metabolomics of 305 Sansui duck breast muscles detected 4,729 metabolic features (459 annotated) and identified 136 sex-differential metabolites enriched in fatty acid and amino acid metabolism pathways. Metabolite-based genome-wide association studies (mGWAS) further identified 355 significant LD-independent SNPs and 267 potential candidate genes associated with 103 metabolites. The signal peaks for amino acid metabolites were mainly concentrated on chromosomes 1, 5, and 20. A QTL on chromosome 2 (63.90-64.10 Mb), containing candidate genes ZNF407, CNDP1, and CNDP2, was identified for three methylglyoxal derivatives, with the lead SNP (chr2: 63928783) accounting for about 18.3 % of their variance. Additional QTLs on chromosomes 5, 20, 1, and 24 were associated with Carnosine, N-Acetylhistidine, Acetylcholine, N-Acetyl-L-aspartic acid, 11b-PGF2a, and 12(S)-HpETE. These intervals harbor BBOX1 and ACACA, two key rate-limiting enzymes in fatty acid metabolism. Our results revealed the genetic basis of breast muscle metabolites in Sansui ducks and identified associated genetic loci and candidate genes. These findings deepen our understanding of muscle metabolism and provide valuable insights for improving meat quality and nutritional breeding. - Source: PubMed
Publication date: 2026/03/18
Feng YulongXu MengruYu XintongYang ZhaoQi JingjingXi YangGuo ShihaoHan XuLi MeijuanLu YuxiDai GuotaoTian ChengchengZhao YuHuang AnqiLi LiangLiu Hehe - Evidence suggests that muscle activity can affect muscle carnosine, but the results are mixed. To address this question, we investigated muscle carnosine under two extremes of the muscle activity-inactivity spectrum. Forty-five male Wistar rats were divided into three groups: immobilization ( = 16), SHAM control ( = 14), and immobilization + exercise ( = 15). In the immobilized groups, one side was submitted to a sciatic nerve sectioning surgery, with the opposite side being submitted to a SHAM control surgery, creating four experimental conditions: denervated (DEN), SHAM active control (SHAM), denervated + exercise (DEN + Ex), and SHAM + exercise (SHAM + Ex). The immobilization period was 12 wk, and the swimming training period was 10 wk (4 times per week, up to 30 min per session). The (TA) and soleus muscles from both sides were assessed for carnosine and anserine contents, total histidine-dipeptides (HCDs), cross-sectional fiber area (CSA), and fiber type distribution. Contractile function was determined ex vivo in the , and the expression of the , , and genes was determined with real-time polymerase chain reaction in TA. Physical inactivity drastically reduced muscle mass, contractile function, and fiber CSA. Long-term postdenervation muscle paralysis reduced muscle carnosine and anserine content, which was not dependent on diet, age, sex, or fiber type. This demonstrates that muscle inactivity is a strong modulator of muscle HCD content, at least under extreme conditions. Gene expression was not significantly altered in any of the experimental conditions. Exercise training, on the other hand, did not affect muscle HCDs and may be a less potent regulator of muscle HCD content. This study demonstrated that an extreme model of muscle inactivity in rats (i.e., 12 wk of hindlimb paralysis following denervation) resulted in a substantial decline in muscle carnosine and anserine, which occurred irrespective of fiber type shift. Conversely, exercise training had no effect on histidine-dipeptide content. These findings, along with previously published studies, reinforce the notion that muscle inactivity is an important modulator of histidine-dipeptide homeostasis in skeletal muscle. - Source: PubMed
Publication date: 2026/02/23
Santana Amanda RomualdoVargas Bianca SciglianoBechara Luiz Roberto GrassmannFormalioni AndressaMöller Gabriella BerwigRodrigues Maria Rita de CamargoPereira Wagner Ribeiroda Silva Beatriz CristinaDos Prazeres Silva KarolineCury Diego PulzattoRoschel HamiltonMoriscot Anselmo SigariFerreira Julio C BMedeiros Marisa Helena Gennari deGonçalves Livia de SouzaArtioli Guilherme Giannini - In the context of increasing freshwater scarcity and the effects of climate change on aquatic environments, developing salt-tolerant fish strains has become a critical strategy for sustainable aquaculture. The limited availability of suitable species for saline-water aquaculture poses a significant challenge, severely impacting the development of the fishery economy. This study investigated the effects of 24 h exposure to freshwater, and seawater at salinities of 4, 9 ppt and 12 ppt, on juvenile grass carp (Ctenopharyngodon idella). Following these exposures, liver tissues were collected to assess physiological and biochemical indicators, as well as transcriptional and metabolic responses. Histological examination revealed that liver structure was compromised after 24 h of exposure to 9 ppt and 12 ppt salt stress. Concurrently, we observed a decrease in the levels of Superoxide Dismutase (SOD), whereas the levels of Malondialdehyde (MDA) exhibited an increase. Salinity exposure significantly altered the levels of 120 metabolic products (67% of which were lipid and lipophilic molecules) and the transcriptional expression of 1005 genes. Metabolomic analysis indicated that most of the significantly different metabolites were associated with the metabolism of lipids and amino acids. Transcriptome analysis revealed significant enrichment of 20 metabolic pathways, including glutathione metabolism, lipid digestion and absorption, bile secretion, glycerolipid metabolism, and the tricarboxylic acid cycle. Comprehensive multi-omics analysis revealed significant alterations in key metabolic pathways, including glycerophospholipid metabolism, α-linolenic acid metabolism, histidine metabolism, and β-alanine metabolism, along with several vital genes such as HO-1, NQO1, GCLM, and GSS, under salt stress. These changes closely correlate with variations in cellular membrane lipid composition and antioxidant activity. Further analysis demonstrated that acute salt stress induces oxidative damage in the liver, leading to lipid imbalance and oxidative stress. This is evidenced by impaired antioxidant function and disruptions in amino acid and fatty acid metabolism. Moreover, carnosine synthesis in liver tissue occurs via the activation of histidine and β-alanine metabolic pathways, leading to the upregulation of CNDP2. This process plays a crucial role in regulating lipid metabolism and redox homeostasis, effectively mitigating the damage caused by acute salinity stress. In summary, these findings provide a deeper understanding of the molecular mechanisms underlying salt stress responses in grass carp and offer valuable insights for the breeding of salt-tolerant strains of grass carp. - Source: PubMed
Publication date: 2026/01/23
Fang XiufengWang YuexuanYu RenjieTang DekunLi ZihanQin QiweiWei Shina - Nonmuscle invasive bladder cancer (NMIBC) represents a significant clinical challenge due to its high recurrence and progression rates. We aimed to characterize proteomic differences between matched pairs of tumor and control bladder tissues in NMIBC to identify potential biomarkers and underlying molecular mechanisms. : Data-independent analysis proteomics experiments were conducted in paired samples from 45 patients with NMIBC, comprising 45 tumor and 45 control tissues. Tumor and nontumor results were compared using a paired Student's test. Proteins detected in at least 50% of the samples were used. : A total of 188 differentially abundant detected proteins were identified, along with 11 proteins exclusively detected in tumor tissues, including SPINT1, TXNDC12, GTF2F1, COPZ1, RS25, PTK2, LSR, SNRNP40, NCOA5, SEC63, and CD2AP. The protein interaction network analysis among this set of proteins revealed AGR2, FLNA, TPM1, and CALD1. Additionally, CNDP2 and CTSD expression were inversely correlated with tumor recurrence and progression risk respectively, while EPS8L2 and KRT7 levels were associated with tumor staging. : Our study identified specific proteins as potential NMIBC biomarkers and drug targets. The identified proteins, particularly those linked to tumor recurrence and staging, warrant further validation to assess their clinical utility in NMIBC diagnosis, prognosis, and treatment strategies. - Source: PubMed
Publication date: 2026/01/26
Silva Tiago AparecidoViana Luciana GodoyCarvalho Valdemir MelechcoBertolla Ricardo PimentaAntoniassi Mariana PereiraAzevedo Hatylas