Ask about this productRelated genes to: AQP3 antibody
- Gene:
- AQP3 NIH gene
- Name:
- aquaporin 3 (Gill blood group)
- Previous symbol:
- -
- Synonyms:
- GIL
- Chromosome:
- 9p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1994-07-25
- Date modifiied:
- 2019-04-23
Related products to: AQP3 antibody
Related articles to: AQP3 antibody
- Functional constipation is a common gastrointestinal disorder that impairs quality of life. Although probiotics have shown benefits in constipation, whether live and heat-killed forms of the same strain act through similar mechanisms remains unclear. This study compared the effects of live and heat-killed K56 in experimental constipation. - Source: PubMed
Publication date: 2026/09/12
Zhao ZhiLi XinyiHe JianWang RanLan LianhangHe JingjingGuo JieLi YixuanZhao WenLi Xiaoxia - Glioblastoma (GBM) frequently recurs after temozolomide (TMZ) therapy and exhibits substantial redox plasticity. Our previous work showed that the effects of hydrogen peroxide (HO) vary with its level and between parental and TMZ-resistant GBM cells. Succinate dehydrogenase subunit D (SDHD), a membrane-anchoring component of mitochondrial complex II, is positioned at the interface of electron transport and redox homeostasis, but its regulation in GBM remains unclear. We therefore examined whether SDHD expression changes across distinct HO-responsive contexts involving aquaporins (AQPs) and AKT. TCGA transcriptomic analysis showed higher SDHD mRNA expression in WHO grade IV than in grade II/III gliomas, whereas paired primary/recurrent high-grade glioma samples showed heterogeneous SDHD changes at recurrence. TMZ reduced SDHD, and SDHD knockdown decreased intracellular reactive oxygen species. Combined redox perturbation reduced SDHD, whereas AKT inhibition restored SDHD expression. Under receptor-associated signaling conditions, EGFRvIII expression or CXCL12 stimulation increased measured HO together with AQP3, AKT Ser473 phosphorylation, and SDHD. In TMZ-resistant cells, pharmacological perturbation of aquaporin-associated signaling decreased AQP9 together with AKT Ser473 phosphorylation and SDHD. An AQP3-targeting compound further reduced cell density when combined with TMZ. Together, these findings suggest that changes in SDHD expression are consistent with a compensatory redox response in GBM cells and that its regulation varies with the nature of HO-associated signaling during TMZ-related stress. - Source: PubMed
Publication date: 2026/09/05
Tsai Kuen-JangChen Kuan-TsoTsai Chin-ChuanHong Zi-XuanQiu Li-YingLiu Chan-ChuanChang Kwang-YuChen Pin-YuanChien Chia-Hung - Genome-wide association studies (GWAS) have uncovered multiple loci associated with atopic dermatitis (AD), although mechanistic understanding remains limited. Here, we examine a risk variant in the aquaporin 3 (AQP3) promoter and propose a mechanistic link between genotype to altered autophagy, skin barrier integrity, and wound healing in AD. A GWAS involving 1261 AD cases and 4062 controls of Chinese ancestry from the Singapore-Malaysia cohort was performed. Linkage disequilibrium (LD) analysis and cis-eQTL datasets were used to assess regulatory associations. Functional effects of promoter variants were validated using in vitro luciferase assays and ex vivo transcriptomic profiling. In vitro experiments in HaCaT keratinocytes investigated the effect of siRNA-mediated AQP3 knockdown on autophagy, skin barrier integrity, and wound closure. A discovery GWAS identified significant association between AD and SNP rs12555686 (p = 1.67 × 10, OR = 1.89), located in the promoter of AQP3. Two additional SNPs in strong LD (rs2231225, rs3758279) formed a major haplotype associated with reduced AQP3 expression. Reduced AQP3 expression was associated with decreased autophagy markers (Beclin1, ATG5, ATG12, GABARAPL2, LAMP2, ATG4C), increased senescence markers (p21, GADD45A, SERPINE1), and elevated inflammatory cytokines (IL8, IL1B, TNF). Lastly, AQP3 knockdown in HaCaT keratinocytes reduced steady-state LC3B-II/LC3B-I ratio, reduced occludin expression, and delayed wound healing in vitro. Promoter variants in AQP3 link genetic susceptibility to gene signatures consistent with altered autophagy, cellular senescence, and inflammation in AD. These findings highlight AQP3 as a potential modulator of both epithelial and immune-related mechanisms in AD pathogenesis. - Source: PubMed
Lim Yi Ying ElizaSio Yang YieLam Terence Yin WengSay Yee-HowReginald KavitaChew Fook Tim - To improve the stability of the natural bioactive substance royal jelly (RJ), this study prepared RJ-loaded sodium alginate nanoparticles (RJ-SANPs) via Ca-mediated ion-gelation method. SEM and TEM showed predominantly spherical nanoparticles. FTIR spectroscopy confirmed the formation of hydrogen bonds between RJ and SA, while XRD analysis revealed the coexistence of crystalline RJ domains with the amorphous SA matrix, together confirming its successful encapsulation. Moreover, the RJ-SANPs showed hydrodynamic diameter of 532.63 ± 64.22 nm to 662.73 ± 63.65 nm and its encapsulation efficiency (EE) ranges from 42.33% to 58.62%. The in vitro release of 10-HDA from RJ-SANPs followed a Fickian diffusion mechanism, as described by the Korsmeyer-Peppas model. DSC results indicated that SA's "egg-box" structure enhanced the thermal stability of RJ by altering its thermal behavior. The 1:1 RJ-SANPs exhibited good biocompatibility toward HaCaT and HFF-1 cells. It increased type I collagen content in HFF-1 cells in a concentration-dependent manner, reaching 593.64 ng/mL at 0.025% (v/v) (p < 0.05), and also showed antioxidant activity with a maximum ABTS radical scavenging rate of 62.10%, as well as antibacterial activity against E. coli, P. fluorescens, B. cereus, and S. aureus. However, the low concentrations tested did not significantly upregulate AQP3 expression, which might be attributed to the dose-dependent bioactivity of RJ-SANPs. They retained stability at 4 °C, pH 3.0-7.0, and 0-300 mmol/L NaCl. The work confirmed that SA encapsulation enhances RJ stability while preserving key bioactivities, suggesting the potential of RJ-SANPs as antioxidant and collagen-promoting functional ingredients for skin-care applications. - Source: PubMed
Publication date: 2026/09/10
Li LeiQuan ShenyuanDu LipengLi HaoTao JialiYu JingHe JiaxinMei ZaojuFang Xiaoming - Constipation is a common gastrointestinal disorder, which impairs the quality of life. This study investigated the ameliorative effects of 6'-sialyllactose (6'-SL) in a loperamide-induced murine model of constipation and associated depressive-like behaviors. Constipation was induced in mice by loperamide and subsequently treated by administering 6'-SL at doses of 100 or 300 mg per kg body weight for 14 days. The ameliorative effects of 6'-SL were evaluated through defecation-related parameters, histopathological and immunohistochemical analyses of colonic and hippocampal tissues, biochemical assays, behavioral tests, gut microbiota analysis, and short-chain fatty acid (SCFA) levels. The results showed that 6'-SL alleviated loperamide-induced constipation by improving defecation-related parameters, colonic histopathology, gastrointestinal neurotransmitter levels, and AQP3 and SCF/c-Kit levels. Moreover, 6'-SL ameliorated constipation-associated depressive-like behaviors and hippocampal alterations while modulating the gut microbiota composition and increasing the SCFA levels. Spearman's correlation analysis suggested that the alleviation of constipation and depressive-like behaviors may be associated with alterations in the gut microbiota and SCFA levels upon 6'-SL intervention. These results provide a theoretical and practical basis for developing functional foods and biomedical products based on 6'-SL. - Source: PubMed
Publication date: 2026/09/09
Song MingjinZhou BingZhang ShiruiLiu YingyingTian ZihaoZhang XuguangXie QinggangMa JiageRen Qiqi