eNOS Blocking Peptide
- Known as:
- eNOS Blocking Peptide
- Catalog number:
- 33r-10759
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- eNOS Blocking Peptide
Related genes to: eNOS Blocking Peptide
- Gene:
- NOS3 NIH gene
- Name:
- nitric oxide synthase 3
- Previous symbol:
- -
- Synonyms:
- ECNOS, eNOS
- Chromosome:
- 7q36.1
- Locus Type:
- gene with protein product
- Date approved:
- 1993-08-23
- Date modifiied:
- 2016-10-05
Related products to: eNOS Blocking Peptide
Related articles to: eNOS Blocking Peptide
- Vascular cognitive impairment (VCI) is a vast disease with chronic cerebral hypoperfusion (CCH) being the major driver. In our previous study, we have verified that Da ChuanXiong (DCX) formula could alleviate VCI, and obviously upregulate the decreased cerebral blood flow, indicating its potential benefits on vascular remodeling. This study aims to explore the pro-angiogenic and vasodilatory effects of DCX formula after CCH injuries, and clarify its pharmacodynamic substances and potential mechanisms. The rat model of CCH was established by bilateral common carotid artery occlusion. CD31 immunofluorescent staining and quail chorioallantoic membrane (qCAM) experiments were conducted to assess the angiogenic effect of DCX. Isolated vascular ring experiments and NO assay in qCAM were performed to evaluate the vasodilatory effect of DCX. Then we analyzed the chemical components absorbed in the blood of DCX under CCH conditions by UPLC-Q-TOF/MS, and further combined network pharmacology, molecular docking to predict the potential mechanisms and effective components. Western blot was performed in CCH rats to verify the possible mechanisms and in vitro pharmacodynamic verification experiment was conducted for innovative active ingredients in DCX. Our results revealed that DCX obviously increased the number of CD31 cells in the hippocampus and brain cortex of CCH rats, and significantly increased the vascular areas in qCAM. DCX significantly dilated the pre-constricted vascular ring and increased NO contents in qCAM. Besides, Senkyunolide A, Butylphthalide, Ferulic acid, Senkyunolide I, Levistilide A, Ligustilide, Senkyunolide H and Gastrodin were identified as the 8 major blood-absorbed compounds of DCX under CCH condition. Next, network pharmacology and molecular docking revealed NOS3 and PI3K/Akt signaling as the critical targets and pathway of DCX improving vascular remodeling against CCH injuries, which was further validated by western blot. Moreover, we firstly discovered that Senkyunolide A and Senkyunolide H could obviously promote cell migration and enhance the tube formation ability of HUVECs in vitro. Taken together, DCX formula effectively enhanced the angiogenesis and vasodilation via activating PI3K/Akt/eNOS signaling, which might be the important pathways for DCX promoting vascular remodeling against CCH injuries. Notably, Senkyunolide A and Senkyunolide H were firstly identified as innovative components responsible for the pro-angiogenic effects of DCX. - Source: PubMed
Publication date: 2026/09/03
Gao JialeLou TianyuHuang BeibeiGuo XiuyingWu HaoLiu JieLi YuetingFeng MenghanLiu LirongXiao Hongbin - γ-Aminobutyric acid (GABA)-producing lactic acid bacteria are promising natural alternatives for hypertension management. Here, we isolated a novel high GABA-yielding strain, Lactobacillus buchneri XBMU-C1 (1.45 g/L), from traditional homemade sauerkraut. The strain exhibited excellent tolerance to acid (83.6% survival at pH 3.0), bile salts (85.1% survival at 1 g/L after 4 h), and simulated gastrointestinal fluid (12.2% survival). In an Nω-Nitro-L-arginine methyl ester (L-NAME) induced hypertensive rat model, oral administration of XBMU-C1 fermentation broth for 6 weeks dose-dependently reduced mean arterial pressure (MAP), with the high dose (150 mg·kg·d) lowering MAP by 22.15 mmHg. Mechanistically, the fermentation broth significantly decreased serum levels of angiotensin-converting enzyme (ACE) and angiotensin II (Ang II), while increasing serum nitric oxide (NO) content. Western blot analysis revealed upregulated protein expression of phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), and endothelial nitric oxide synthase (eNOS) in vascular tissues. Histopathological examination showed that the fermentation broth alleviated hypertension-induced myocardial injury, vascular smooth muscle hyperplasia, and intestinal villus damage. Collectively, Lactobacillus buchneri XBMU-C1 fermentation broth exerts antihypertensive effects through dual modulation of renin-angiotensin-aldosterone system (RAAS) suppression and PI3K/Akt/eNOS-mediated NO enhancement, offering a safe and effective natural intervention for hypertension. - Source: PubMed
Publication date: 2026/07/18
Gao DandanWang ChenchenChen YingWang JiwenMei JunyaoCao YujieYang XuankangKang ShangyunMa JinyongLi Ping - Chronic obstructive pulmonary disease (COPD)-associated sarcopenia is a critical comorbidity associated with high morbidity and mortality, driven by chronic systemic inflammation, oxidative stress, and proteometabolic dysregulation. The (HQ-RS) herbal pair is a traditional remedy for consumptive diseases; however, its molecular mechanisms against this comorbidity remain undefined. - Source: PubMed
Publication date: 2026/08/27
Wu XiaowenLi JiaxiangHe JiananYu ZhiyueGao MingGeng Limei - Uncoupling protein 2 (UCP2) is expressed in various tissues throughout the body, but its expression in the spleen exceeds that of other organs. However, the precise function of UCP2 for spleen physiology is unclear. The spleen acts as a hub connecting the nervous system and immune system to cardiovascular and metabolic diseases. Here, we analyzed the impact of hypertension on the spleen and the role of UCP2 in this process. Experiments were performed with UCP2-knockout rats and their wild-type littermates. Hypertension was induced by administering the nitric oxide inhibitor L-NAME via tap water. Genetic depletion of UCP2 increased spleen size (splenomegaly) and strongly impaired the expression of genes coding for mitochondrial proteins. Among them, genes coding for proteins involved in oxidative metabolism, such as pyruvate dehydrogenase alpha 1, and the detoxification of reactive oxygen species were down-regulated. Collectively, these alterations in metabolism favor glycolysis and proliferation. Moreover, NOS3 was among the strongest down-regulated genes in UCP2 rats, and the inhibition of nitric oxide synthase by L-NAME mimicked large parts of the expression profile. Neither the depletion of UCP2 nor L-NAME-induced hypertension or combinations thereof affected chronic inflammation. In summary, UCP2 controls fuel consumption in splenic cells in a nitric-oxide-dependent way. - Source: PubMed
Publication date: 2026/07/30
Wagner LeaSchreckenberg RolfItani NadjaSato TsuneshiroSperhake JuliaCesar YvaSchlüter Klaus-Dieter - Vascular aging is driven by a self-sustaining interplay between oxidative stress and chronic low-grade inflammation, collectively referred to as inflammaging. Diosmin, a citrus-derived flavonoid with antioxidant and anti-inflammatory properties, has not previously been investigated in this context. Young (10-week-old) and aged (40-week-old) male Sprague-Dawley rats received diosmin (50 mg/kg/day) or vehicle for 3 months. Vascular function was assessed in mesenteric resistance arteries using pressure myography, while oxidative stress was evaluated by dihydroethidium and mitoSOX staining. Circulating malondialdehyde (MDA), tumor necrosis factor (TNF), lipopolysaccharide-binding protein (LBP) vascular expression of , , and were measured. Aging was associated with impaired endothelium-dependent vasorelaxation, increased vascular DHE and MitoSOX fluorescence and elevated circulating MDA and LBP levels. Chronic diosmin supplementation partially restored endothelial function. The improvement in endothelial function was consistent with a partial preservation of NO-dependent endothelial function, although NO production and bioavailability were not measured directly. Diosmin also significantly reduced vascular DHE and MitoSOX fluorescence in aged animals. These effects were accompanied by decreased plasma MDA and LBP levels, whereas TNF remained unchanged. Diosmin did not significantly affect , or gene's expression, but it attenuated the age-related upregulation of . Overall, diosmin attenuated age-associated vascular oxidative stress and partially improved endothelial function in aged rats. Although the reduction in circulating LBP suggests a possible effect on selected systemic signals associated with endotoxin exposure, additional inflammatory markers and mechanistic studies will be required to determine whether diosmin modulates vascular inflammaging. - Source: PubMed
Publication date: 2026/08/07
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