Ask about this productRelated genes to: BMP14 protein
- Gene:
- GDF5 NIH gene
- Name:
- growth differentiation factor 5
- Previous symbol:
- -
- Synonyms:
- CDMP1, BMP14
- Chromosome:
- 20q11.22
- Locus Type:
- gene with protein product
- Date approved:
- 1997-12-05
- Date modifiied:
- 2016-10-05
Related products to: BMP14 protein
Related articles to: BMP14 protein
- Intervertebral disc degeneration is associated with loss of nucleus pulposus (NP) cell phenotype and extracellular matrix, both processes linked to changes in cytoskeletal contractility and cell shape. Here, we tested whether microenvironment-specific modulation of RhoA signaling can restore NP-like morphology and gene expression in NP cells cultured in 2D and in 3D alginate. In 2D monolayer culture, where cells are spread and mechanically activated, pharmacologic inhibition of RhoA with CT04 reduced RhoA activity, decreased actomyosin contractility gene expression, and shifted morphology toward a smaller, more circular phenotype. Bulk RNA sequencing showed that CT04 treatment increased expression of NP phenotypic and matrix-related genes including , , , and while decreasing expression of catabolic and fibroblast-associated genes including and , consistent with enrichment of extracellular matrix pathways. In contrast, RhoA activation with CN03 in 2D culture increased actin and phosphorylated myosin light chain intensity but produced limited phenotypic improvement. In 3D alginate, which minimizes integrin-mediated adhesion, baseline actomyosin markers were reduced relative to 2D culture. In alginate, RhoA activation with CN03 increased the amount of actin, phosphorylated myosin light chain, and actomyosin gene expression, yet also promoted a more compact, circular morphology and increased NP markers, including and with repeated dosing. Across culture conditions, increased cell roundness was consistently associated with increased expression, indicating strong coupling between cytoskeletal state, morphology, and NP matrix programs. Together, these findings demonstrate that RhoA pathway perturbation can promote NP phenotypic gene expression in both 2D and 3D culture, but the direction of optimal modulation depends on the microenvironment, supporting RhoA signaling as a context-dependent therapeutic target for disc regeneration. - Source: PubMed
Publication date: 2026/04/07
Bond GabriellaKim Min Kyu MLisiewski LaurenJacobsen TimothyChahine Nadeen - Many studies have reported the role of genetic polymorphisms in osteoarthritis (OA). While, there lacks of a comprehensive synthesis of bibliometric trends on this topic. This study aims to summarize global research progress and identify current hotspots by a bibliometric analysis. - Source: PubMed
Publication date: 2026/04/13
Guo RuiAizezi AihaitiJi ZheCao ZhengyiTong ShanyingNing Kai - Focal damage to articular cartilage incurred during joint injuries frequently progresses to post-traumatic osteoarthritis (PTOA) due to the limited intrinsic repair capacity of cartilage. Chondrogenic progenitor cells (CPCs) residing within the cartilage can contribute to repair if effectively recruited and activated. Early interventions that enhance CPC homing and their subsequent chondrogenesis offer a regenerative strategy to prevent PTOA progression, addressing the current lack of effective early clinical therapies. GDF5 stands out as a key protein involved in cartilage development, yet its potential to mobilize CPC-mediated regeneration remains underexplored. - Source: PubMed
Publication date: 2026/04/14
He RuiGanesh VenkateswaranPhruttiwanichakun PornpojMartin James ASeol DongrimSalem Aliasger K - Developmental dysplasia of the hip (DDH), a morphological abnormality of the hip joint, is a well-recognized risk factor for hip osteoarthritis (OA). Much remains unknown about the genetic factors of DDH and its subtypes. To further understand its genetic basis, we conducted genome-wide association studies (GWASs) using a total of 1 085 Japanese DDH cases (including 788 hip dysplasia cases without dislocation and 297 cases with dislocated hip) and 24 000 controls. Additionally, we meta-analyzed with United Kingdom (UK) DDH GWAS and the largest hip OA GWAS to date. We identified three genome-wide significant novel loci, COL11A2, CALN1 and TRPM7, associated with hip dysplasia without dislocation. None of these signals were significant in dislocated hips, and additionally two of the signals had an opposite direction of association, suggesting distinct genetic architectures between the subtypes. The Japanese DDH GWAS identified five associated loci (VEGF-C, FOXC1, SMC2, SLC38A4, and TRPM7), and the trans-ancestry meta-analysis with UK revealed two loci (COL11A1 and GDF5) supported by strong trans-ancestry genetic correlation (r = 1.0). In total, nine loci were identified for DDH and its subtypes, with hip dysplasia without dislocation showing distinct genetic signals from hip dislocation. The meta-analysis of DDH and hip OA identified five novel signals for hip OA. Susceptibility loci and heritability enrichment analyses implicated pathways involving bone formation, collagen type XI trimer, and chondrocyte development, as well as their gene regulation, in DDH. These findings enhance understanding of the genetic architecture and biological pathways underlying DDH, providing new insights into its relationship with OA. - Source: PubMed
Publication date: 2026/03/31
Yoshino SoichiroChen ShiboYamaguchi RyosukeKurakazu TaishiHatzikotoulas KonstantinosKoike YoshinaoInoue DaisukeKohno YusukeSasaki KanChoe HyonminBaba ShojiHara ToshihikoIto JujiOkuzu YaichiroShiomoto KyoheiNakamura TomoyukiKoyano GakuShimizu TomohiroKinoshita KoichiTakahashi EijiUtsunomiya TakeshiHara DaisukeSato TaishiKawahara ShinyaKaneuji AyumiYamamoto TakuakiTakahashi DaisukeJinno TetsuyaKawano TsutomuGoto KojiTakagi MichiakiMawatari TaroInaba YutakaNakamura TetsuroKabata TamonHamai SatoshiMotomura GoroSoutham LorraineWilkinson J MarkZeggini EleftheriaIkegawa ShiroNakashima YasuharuTerao Chikashi - The early gonads of mammals contain primordial germ cells (PGCs) and gonadal somatic cells. In females, the gonadal somatic cells promote the specification of PGCs into oogonia and their entry into meiosis, which is crucial for oogenesis. Although single-cell transcriptome sequencing technology holds significant advantages in cell type identification, its inability to resolve spatial positional information substantially hampers research on communication mechanisms between germ cells and adjacent somatic cells. Here, we utilized high-resolution spatial transcriptomic technology to dissect the spatial dynamics of various cell types during the specification of PGCs into oogonia and their entry into meiosis in porcine gonads. We clarified the spatial localization of two waves of granulosa cells in the supporting cell lineage and their roles in regulating germ cell development. Furthermore, we found that interstitial and endothelial cells were predominantly located in the medullary region of the early gonads. Notably, cell-cell communication analysis revealed the critical role of BMP signaling (BMP2, BMP4 and GDF5) in driving the specification of PGCs into oogonia and their subsequent entry into meiosis. Our study provides a spatially resolved understanding of the PGC-to-oogonia specification in vivo and offers critical resources for reconstituting oogenesis in vitro. - Source: PubMed
Publication date: 2026/03/30
He PengchengXia WenzheChen TianzhiYan YaxuanGao DengfengTeng YadiZhao TingChen XinzeFeng ZhiqiangLi RunboWang MengKe YuwenHan Jianyong