Ask about this productRelated genes to: MSRA antibody
- Gene:
- MSRA NIH gene
- Name:
- methionine sulfoxide reductase A
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 8p23.1
- Locus Type:
- gene with protein product
- Date approved:
- 1996-12-19
- Date modifiied:
- 2015-08-25
Related products to: MSRA antibody
Related articles to: MSRA antibody
- Antimicrobial resistance (AMR) among Staphylococcus and Mammaliicoccus species is a growing concern in wildlife care settings, as rehabilitation centers may represent contact points among wild animals, captive environments, and human handlers. This study characterized antimicrobial resistance profiles, multiple antibiotic resistance (MAR) indices, and biofilm formation in Staphylococcus spp. and Mammaliicoccus sciuri isolated from wild mammals housed at a wildlife screening and rehabilitation center in Pernambuco, northeastern Brazil. Oropharyngeal and rectal swabs were collected from 84 individuals representing 15 species. Isolates were identified using MALDI-TOF MS, and susceptibility to oxacillin, cefoxitin, clindamycin, erythromycin, gentamicin, norfloxacin, and tetracycline was assessed by disk diffusion. Multidrug resistance (MDR) and MAR indices were determined, and the resistance genes mecA, mecC, norA, msrA, tet(38), and tetM were investigated by PCR. Bacterial growth on mannitol salt agar was obtained from samples collected from 63/84 (75.0%) animals, yielding 63 confirmed isolates. Mammaliicoccus sciuri was the most frequently identified species, accounting for 29/63 (46.0%) isolates, followed by Staphylococcus aureus, Staphylococcus felis, and Staphylococcus simulans, with 5/63 (7.9%) isolates each. Overall, 50/63 (79.4%) isolates were resistant to at least one antimicrobial category, and 13/63 (20.6%) were classified as MDR. Resistance was most frequently observed to oxacillin (68.3%), followed by cefoxitin (28.6%), erythromycin (27.0%), tetracycline (25.4%), clindamycin (22.2%), norfloxacin (11.1%), and gentamicin (11.1%). MAR indices ranged from 0.00 to 1.00, with a mean value of 0.28. Resistance genes were detected in a subset of isolates, including mecA (4/63; 6.3%), norA (2/63; 3.2%), and msrA (1/63; 1.6%), whereas mecC, tet(38), and tetM were not detected. Biofilm formation was observed in 33/63 (52.4%) isolates, predominantly among weak and moderate producers. These findings provide baseline evidence supporting systematic microbiological surveillance, antimicrobial stewardship, and biosafety measures in wildlife rehabilitation facilities within a One Health framework. - Source: PubMed
Publication date: 2026/08/26
Parente de Sá Barreto Maia Leite DennyPerrelli Sarmento TaoanaVieira da Silva ValdirMartins Trindade Gonçalves LucileneRaysa Fernandes de Oliveira PollyanneSilva Santos RafaelaUchôa Cavalcanti Moreira da Silva Maria Eduardade Oliveira Alves Pinto GustavoGivanildo da Silva JoséRosa Fernandes Beraldo KarolinaAparecida Juliano MariaAparecido Mota Rinaldo - Mobile phones used in healthcare environments may serve as overlooked reservoirs facilitating the transmission of clinically significant pathogens. This study investigated antimicrobial resistance patterns, virulence-associated determinants, and genetic diversity among Staphylococcus aureus isolates recovered from healthcare workers' mobile phones. Phenotypic antimicrobial susceptibility testing, staphylococcal cassette chromosome mec (SCCmec) typing, multilocus sequence typing (MLST), and polymerase chain reaction assays were employed to detect antimicrobial resistance, biofilm-associated, and toxin-encoding genes. Among 349 mobile phones screened, 42 (12%) were positive for S. aureus. Among the isolates, 76.2% were identified as methicillin-resistant S. aureus (MRSA), while 23.8% were methicillin-susceptible S. aureus (MSSA). High-level mupirocin resistance (HLMUPR) and inducible macrolide-lincosamide-streptogramin B (iMLSB) phenotypes were observed in 26.2 and 35.7% of isolates, respectively. Biofilm formation analysis demonstrated that 49.2% of isolates were strong biofilm producers, followed by intermediate (30.8%) and weak producers (15.4%). Of the 42 isolates examined, 12 (28.6%) harbored the pvl gene, and 10 (23.8%) harbored the tst gene. The most frequently detected resistance gene was erm(B) (33.3%), followed by msrA (19%) and erm(C) (16.7%). All isolates harbored the adhesion genes fnbA and fnbB. Additional biofilm- and adhesion-associated genes included clfA and clfB (85.7%), cna and fib (38.1%), icaA (35.7%), icaD (33.3%), sdrD (28.6%), sdrC, sdrE, eno, and bap (21.4% each), while ebpS was detected in 4.8% of isolates. MLST analysis revealed distribution across six clonal complexes, predominantly CC8 (42.9%), followed by CC5 (21.4%), CC59 (11.9%), CC22 (11.9%), CC1 (7.1%), and CC398 (4.8%). Panton-Valentine leukocidin (PVL)-positive isolates were mainly associated with ST8-SCCmec IV/t008 and ST22/t1869 lineages, whereas toxic shock syndrome toxin (TSST-1)-positive strains belonged primarily to ST239-SCCmec III/t037 and ST59-SCCmec IV/t437. High-level mupirocin-resistant isolates carrying mupA were distributed among several epidemic clones, including ST5-SCCmec IV/t002 (36.4%), ST8-SCCmec IV/t008 (27.2%), ST239-SCCmec III/t037 (18.2%), and ST22/t1869 (18.2%). Overall, the findings demonstrate substantial circulation of multidrug-resistant and virulence-associated S. aureus lineages on healthcare workers' mobile phones, underscoring their potential role in pathogen dissemination and emphasizing the necessity for targeted infection prevention and mobile device decontamination strategies in healthcare settings. - Source: PubMed
Publication date: 2026/08/17
Goudarzi MehdiNasiri Mohammad JavadDadashi MasoudGoudarzi Hossein - ClpX functions as a component of the ClpXP protease, a conserved intracellular protease that regulates protein turnover, stress responses, and virulence in multiple bacterial species. Our lab has established that clpX is necessary for resistance to cell envelope targeting antibiotics, such as penicillin and daptomycin in Bacillus anthracis Sterne. Previous microarray data identified the msrA/B gene encoding a bifunctional methionine sulfoxide reductase as upregulated in the ΔclpX mutant. Methionine sulfoxide reductases (Msr) repair oxidatively damaged proteins by reducing methionine sulfoxide residues back to methionine. While Msr enzymes are primarily associated with oxidative stress, cell wall antibiotics induce expression of msrA1 and msrB in S. aureus. Here, we investigated the role of MsrA/B in oxidative and cell envelope stress. Our results show that although hydrogen peroxide and paraquat induce msrA/B expression, the ΔmsrA/B strain was not susceptible to either oxidant, whereas the ΔclpX strain was sensitive to both. We also found that loss of msrA/B conferred penicillin-specific sensitivity, but, unlike ΔclpX, increased sensitivity was not seen with other cell wall or cell membrane targeting antibiotics. Inactivation of the catalytic cysteine of either Msr domain of MsrA/B failed to complement, suggesting that the reducing activity of MsrA/B is required for penicillin resistance. These findings indicate that while MsrA/B contributes to penicillin resistance, other proteins in the ClpXP modulon must also play a role in oxidative and cell envelope stress. - Source: PubMed
Pennington Aeron BHona SalinaAustin Josey IWaite Kelsey CStewart Mikaela DMcGillivray Shauna M - Glycosylphosphatidylinositol-specific phospholipase D1 (Gpld1) is a membrane-associated enzyme that modulates diverse cellular processes through the cleavage of glycosylphosphatidylinositol (GPI)-anchored proteins. Although recent studies have linked circulating Gpld1 to exercise-induced rejuvenation, its cell-autonomous role in coordinating redox homeostasis, melanogenesis, and cellular aging has not been fully elucidated. - Source: PubMed
Publication date: 2026/08/11
Lee Si EonKim Moon-Moo - Radiation-resistant microorganisms that survive high doses of ionising radiation serve as valuable models for understanding stress adaptation; however, the genomic determinants underlying extreme radiation tolerance in bacteria from natural environments with high background radiation remain insufficiently characterised. Bacterial isolates from the Chavara-Neendakara HBRA (Kerala, India) were evaluated for desiccation tolerance, and the desiccation-resistant isolates were subsequently exposed to gamma irradiation (1-10 kGy) using a Co source. Isolates were identified through 16S rRNA sequencing, morphologically characterised by FE-SEM, and screened for antibiotic susceptibility. The highly radiation-resistant strain underwent whole-genome sequencing via Oxford Nanopore Technology, with De novo assembly, polishing, and genome annotation. Four bacterial isolates (Micrococcaceae and Paenibacillaceae) exhibited D values of 1-7 kGy, including one multidrug-resistant strain; no endospores were observed in the Paenibacillus isolate under the tested conditions. Paenibacillus sp. HBRA004 survived 10 kGy gamma radiation, exceeding all previously reported HBRA isolates by over fourfold. Its 5.0 Mbp genome (GC = 48.27%, ≥ 99% completeness) encodes five mechanistically independent DNA repair pathways; homologous recombination (recA, recN, radA), base excision repair (mutM, mutY, mutT), mismatch repair (mutL, mutS), nucleotide excision repair (uvrA, uvrB, uvrD), and non-homologous end joining (ku, ligD), alongside a redundant antioxidant network comprising triple-copy Fe/Mn-family superoxide dismutases and ahpC peroxiredoxin. A thioredoxin system (trxA, trxB, msrA) and manganese uptake via mntH may contribute to further layers of ROS defence. Their specific contribution to the HBRA004 phenotype remains to be experimentally and comparatively validated. These findings represent the first genomically characterised 10 kGy-resistant bacterial isolate from the Chavara-Neendakara HBRA, establishing a new benchmark for radiation tolerance within this ecologically significant environment. Pathway depth, gene copy amplification, and Mn/Fe homeostasis appear to be candidate mechanisms contributing to high-level radiation tolerance, consistent with patterns in other radiation-resistant taxa, though their contribution requires functional validation. - Source: PubMed
Publication date: 2026/08/06
Thomas AnuMahajan MeghaBabu Seetha SAnoop GayathriNithya T GBabu Pothireddy Raghu