Ask about this productRelated genes to: AKT1 antibody
- Gene:
- AKT1 NIH gene
- Name:
- AKT serine/threonine kinase 1
- Previous symbol:
- -
- Synonyms:
- RAC, PKB, PRKBA, AKT
- Chromosome:
- 14q32.33
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: AKT1 antibody
Related articles to: AKT1 antibody
- Bladder cancer (BCa) is a prevalent malignancy with high recurrence. While gut microbiota metabolites influence BCa progression, the specific bioactive components and underlying mechanisms remain elusive. This study integrates network pharmacology and molecular simulations to identify core metabolites and therapeutic targets. - Source: PubMed
Publication date: 2026/04/29
Qin RuizeLi ChengZhang TianweiWang Yonghua - Pancreatic cancer is a major cause of death and one of the most challenging types of cancer which responds poorly to conventional chemotherapy and has limited therapeutic options. The scenario highlights the urgent need for the development of newer multi-targeting anticancer drugs for pancreatic cancers with higher potency, selectivity and safety profiles. The proposed research was focused on revealing the anticancer potential and mechanistic involvement of naturally occurring sesquiterpenoid nootkatone against pancreatic cancer through an integrative in silico approach. Network pharmacology and systems biology approaches were applied to identify common therapeutic targets for nootkatone that were pathophysiologically associated with the progression of pancreatic cancer. Protein-protein interaction (PPI), Gene Ontology (GO), and KEGG enrichment analyses were executed to validate the target's involvement in the pathophysiology of pancreatic cancer. Molecular docking and dynamics simulations were performed to reveal the binding potential and interactions of the nootkatone with the shortlisted anticancer targets, followed by density functional theory (DFT) analysis, ADMET prediction, and clinical relevance assessment to confirm its electrochemical and physicochemical involvement. A total of 27 overlapping targets were identified with AKT1, MAPK3, IL1β, and COX-2 revealed as the four hub target genes for nootkatone by network pharmacology. Enrichment analyses confirmed the significant involvement of PD-L1/PD-1 immune checkpoint, PI3K-AKT, MAPK, and inflammatory signaling pathways (FDR < 0.05) in the progression of pancreatic cancer. Docking analyses revealed that nootkatone has binding affinity for its targets, especially for MAPK3/ERK1 (- 8.03 kcal/mol) and AKT1 (- 7.35 kcal/mol). MD simulation over 100 ns demonstrated the stable protein-ligand complexes. DFT calculations showed a HOMO-LUMO energy gap of 2.868 eV, indicating moderate chemical reactivity and stability. Nootkatone was found to exert stronger and more stable binding against all four concerned anticancer targets with impressive electrochemical properties. ADMET predictions suggested favourable drug-likeness and Pharmacokinetics. Nootkatone can be a potential multi-targeting agent with anticancer and immunomodulatory properties by modulating the PD-L1/PD-1 immune checkpoint and signalling pathways associated with pancreatic cancer progression. However, these findings are based on in silico analyses and require further validation through in vitro and in vivo experimental studies to develop new plant-based anticancer therapeutics for pancreatic cancer. - Source: PubMed
Publication date: 2026/05/01
Goel KaranSingh Thakur GurjeetMujwar Somdutt - Bacteria-modulated gastric epithelial cells (GECs) play key roles in Helicobacter pylori-associated pathology. Here, we demonstrate both procolonization and proinflammation roles of GEC-derived PPFIA4 in H. pylori infection. PPFIA4 was elevated in GECs from gastric mucosa of H. pylori-infected patients and mice. PPFIA4 could be synergistically induced by H. pylori and IL-33 via the CagA/AP1 pathway. Human gastric PPFIA4 correlated with H. pylori colonization and the severity of gastritis, and H. pylori colonization and inflammation were attenuated in Ppfia4ΔGEC mice. Mechanistically, PPFIA4's SAM1 domain bound domains from CaMK to the first L27 of CASK and subsequently formed a PPFIA4/CASK/AKT1 complex to activate AKT1, resulting in NF-κB activation and MMP1/CXCL3 secretion. This not only led to decreased E-cadherin and ZO-1 by MMP1, thereby promoting gastric mucosal damage to foster H. pylori colonization, but also resulted in increased gastric influx of G-MDSCs via CXCL3-dependent migration, thereby promoting gastritis and impairing H. pylori-specific IFN-γ-producing CD4+ T cell responses to foster H. pylori colonization. Furthermore, we identified a PPFIA4 inhibitor, kira6, which effectively inhibited GEC's MMP1/CXCL3 production and ameliorated gastric H. pylori colonization and gastritis. Overall, PPFIA4 could be a promising therapeutic target, as it collectively ensures H. pylori persistence and promotes gastritis. - Source: PubMed
Publication date: 2026/05/01
Wang PanYou NanTeng Yong-ShengLv Yi-PinTian Wen-QingXu Jing-YuXie RuiWu Jiang-BoYue Geng-YuCheng PingZhang Jin-YuPeng Liu-ShengMao Fang-YuanLuo Shou-LuYang Shi-MingZhao Yong-LiangZhou HongChen WeisanWang BinZhuang Yuan - Nonobstructive azoospermia (NOA) is one of the most severe types of male infertility. Amino acid metabolism (AAM) is strongly associated with various diseases. This study intended to identify biomarkers related to AAM in NOA. Biomarkers were screened from GSE9210, GSE108886, and AAM-related genes through a series of bioinformatics analyses. Then, the diagnostic efficacy of the biomarkers was evaluated. Furthermore, the mechanisms of action of these biomarkers were investigated through enrichment analysis, immune infiltration analysis, construction of regulatory networks, prediction of potential drugs, and prediction of related diseases. Finally, reverse transcription-quantitative polymerase chain reaction was performed for verification. Three biomarkers (AKT1, ASNS, and SHC1) were derived, which demonstrated good diagnostic efficacy for NOA. Meanwhile, AKT1, ASNS, and SHC1 might play significant roles in the development of male germ cells through pathways such as male gamete generation, sexual reproduction, and gamete generation. Immune infiltration analysis revealed these 3 biomarkers were closely associated with T follicular helper cells, resting natural killer cells, and regulatory T cells. And AKT1, ASNS, and SHC1 were regulated by 24 transcription factors and 15 microRNAs. Remarkably, sodium selenite, aflodac, and genistein were commonly predicted by the biomarkers. In addition, lung neoplasms were associated with AKT1, lipoidosis was associated with ASNS, and drug-induced acute liver injury was associated with SHC1. Ultimately, AKT1 and SHC1 were significantly upregulated in NOA. This study identified 3 biomarkers as being associated with NOA, providing valuable clues to help treat and predict NOA. - Source: PubMed
Guo Qing HuaDing Zhong JunLiu Dong MeiRen Xiao ShanChen XiaoYong - Histologically normal mammary tissue from breast cancer patients can harbor significant genetic alterations that could precede visible tumor development and influence disease progression. - Source: PubMed
Publication date: 2026/04/30
Andreou MariaChojnowska KatarzynaFilipowicz NataliaHorbacz MonikaMadanecki PiotrDuzowska KatarzynaŁawrynowicz UrszulaDavies HannaBruhn-Olszewska BożenaKoszyński MikołajDrężek-Chyła KingaJaśkiewicz MaciejJąkalski MarcinKostecka AnnaDrzewiecka-Kłysz MartaNowikiewicz MagdalenaLas-Jankowska ManuelaBała DariuszHoffman JacekŚrutek EwaJankowski MichałJankau JerzyHodorowicz-Zaniewska DianaSzpor JoannaSzylberg ŁukaszZegarski WojciechNowikiewicz TomaszBuckley Patrick GTiemann-Boege IreneMieczkowski JakubKoczkowska MagdalenaDumanski Jan PPiotrowski Arkadiusz