Ask about this productRelated genes to: CD146 antibody
- Gene:
- MCAM NIH gene
- Name:
- melanoma cell adhesion molecule
- Previous symbol:
- -
- Synonyms:
- MUC18, CD146, MelCAM, METCAM, HEMCAM
- Chromosome:
- 11q23.3
- Locus Type:
- gene with protein product
- Date approved:
- 1995-12-18
- Date modifiied:
- 2019-04-16
Related products to: CD146 antibody
Related articles to: CD146 antibody
- Herein, we report a novel series of heterocyclic fluoro-methoxy-phenyl-substituted N-sulfonylpiperidine mono-spiro-1,2,4,5-tetraoxanes (5a-l; 50-93%) that have been successfully synthesised under metal-free and environmentally benign conditions. The inhibitory effects of all derivatives (5a-l) were determined in vitro on Plasmodium falciparum, Trypanosoma brucei, Mycobacterium, HeLa, and A2780 ovarian cancer cell cultures. The heterocyclic 1,2,4,5-tetraoxanes (5a-l; IC = 4.1-16.2 μM) displayed interesting micromolar antiplasmodial potential against chloroquine-resistant FcB1 strains of P. falciparum. Moreover, four representative analogues (5e, 5h, 5k, 5b) were evaluated in Danio rerio larvae, showing no lethality within the tested range, with sublethal morphological alterations observed at higher concentrations. As a result of a complex biological activity assessment, compounds with micromolar antiparasitic and antimycobacterial activities were identified, within an acceptable cytotoxicity range. Notably, the presented study constitutes the first evidence of a non-steroidal monomeric p-OCF-Ph-based 2-fluoro-p-OMe-phenyl ring-substituted N-sulfonylpiperidine mono-spiro-1,2,4,5-tetraoxane (5j; Pf FcB1 IC = 5.5 μM, Tbb IC = 25.6 μM, M. tuberculosis H37Ra MIC = 40 μM) exhibiting efficient multispecies-targeting susceptibilities. - Source: PubMed
Publication date: 2026/04/02
Tiwari Mohit KBakun PawelForest EmmaBola Jean-MichelMurias MarekKucinska MalgorzataZgoła-Grześkowiak AgnieszkaCicha EmiliaMarczak ŁukaszCavalier Jean-FranҫoisGrellier PhilippeGoslinski Tomasz - Here, solution NMR, gas-phase hydrogen/deuterium back-exchange (HDbX)-trapped ion mobility spectrometry (TIMS), electron capture dissociation (ECD), quantum mechanical (QM) calculations, and molecular dynamics (MD) simulations were combined for comprehensive structural elucidation of the lasso peptide syanodin I. NMR and ECD MS/MS confirmed an entangled lasso structure with Gln13 as the plug residue maintaining the lasso thread. A maximal /' ratio at Ala11 () was consistent with multiple long-range NOE correlations, identifying Ala11 in proximity to the macrolactam ring. ECD fragmentation patterns indicated a salt bridge between the C-terminus and the Gln13 side chain. TIMS resolved four distinct IMS bands for [M + 2H] ions of syanodin I and its branched-cyclic analog over a similar collision cross-sectional range. HDX-MS revealed mass shifts of ∼17 and ∼20 deuteriums for the lasso and branched-cyclic forms, respectively, consistent with the folded nature of the branched-cyclic C-terminal region. HDbX-TIMS-MS experiments ( ∼ 0.72 to ∼ 865 ms) resolved at least two distinct conformers within each IMS band, revealing intramolecular interactions inaccessible by TIMS alone. QM calculations determined the HDX rate and number of accessible hydrogens for Pro10 and Gln13; this information was used to inform the MD candidate assignment of the 2D-HDX-TIMS-MS results. This workflow provides a comprehensive framework for probing biomolecular conformational dynamics through complementary solution- and gas-phase approaches. The integration of solution-phase hydrogen/deuterium exchange (HDX) with ion mobility spectrometry-mass spectrometry (IMS-MS) offers powerful structural insights into the conformational dynamics of biological molecules. - Source: PubMed
Publication date: 2026/04/14
Santos-Fernandez MiguelJeanne Dit Fouque KevinKarki UkeshBlond AlainIdir RyaneAsgharihosseinpour NeginZirah SéverineChapagain PremMebel Alexander MHegemann Julian DFernandez-Lima Francisco - Although processing windows have been widely reported for LPBF Ti-6Al-4V, the distinct roles of laser power, scanning speed, and hatch distance remain unclear beyond VED-based comparisons. In this work, the distinct effects of laser power, scanning speed, and hatch distance on the microstructural evolution and mechanical response of laser powder bed fusion (LPBF) Ti-6Al-4V (Ti64) are investigated within a stable processing window with comparisons among different parameter combinations at a comparable VED. A total of 56 processing conditions were designed, and microstructure/texture and properties were characterized by OM/SEM, EBSD, microhardness (HV0.5), and hole-drilling residual stress measurements. Within the selected processing window, prior-β grain morphology, α' martensite thickness, texture, microhardness, and residual stress exhibit distinct sensitivities to different processing parameters. Specifically, lower scanning speeds and smaller hatch distances promote more continuous <001>β epitaxial growth, whereas higher scanning speeds or larger hatch distances produce fragmented prior-β grains. The α' lath thickness shows the strongest dependence on scanning speed with a secondary influence from hatch distance, while laser power mainly provides an overall thermal modulation. Furthermore, the macroscopic α (0002) texture is mainly governed by the β solidification texture, with α-variant selection playing a secondary, amplifying role. In addition, microhardness correlates with α' martensite thickness following a Hall-Petch equation. The peak residual stress is more sensitive to scanning speed, while bulk residual stress varies more significantly with hatch distance. These findings demonstrate that process parameters, in addition to VED, can guide microstructural control and mechanical optimization in LPBF Ti64 alloy. - Source: PubMed
Publication date: 2026/03/10
Shuai YuxinLiu JieZhu JingHuang ZhichaoZha WenhaoYang YiZhang RuifengZhang Kai - Endometriosis is a heterogeneous chronic inflammatory disorder associated with substantial diagnostic delay and limited therapeutic options, highlighting the need of robust non-invasive biomarkers and actionable molecular targets to complement existing low-sensitivity tests. To identify conserved pathogenic mechanisms with translational potential, here, we uniformly reprocessed three independent the Gene Expression Omnibus (GEO) microarray cohorts (GSE7305, GSE25628, and GSE11691) and applied a strict, directionally consistent intersection strategy to identify conserved transcriptional signals. We identified 262 consensus differentially expressed genes enriched for immunity/inflammation, cell adhesion and migration, and angiogenesis, consistent with key biological hallmarks of lesion establishment and persistence. Protein-protein interaction topology prioritized 11 highly connected hub genes (, , , , , , , , , , and ) that likely act as an integrated immune-adhesion-angiogenesis axis. Notably, 63/262 (24%) of the consensus genes were annotated to the extracellular exosome compartment, supporting their translational relevance as liquid-biopsy candidates. Finally, connectivity mapping using the LINCS L1000 framework nominated small-molecule perturbagens predicted to reverse the endometriosis-associated signature, providing a rational starting point for drug-repurposing experiments. In conclusion, this study elucidates a conserved immune-adhesion-angiogenesis axis driven by an 11-gene hub network in endometriosis. These core regulators represent promising candidates for the development of non-invasive liquid biopsies and precision, non-hormonal therapeutics. - Source: PubMed
Publication date: 2026/03/11
Tsai Meng-HsiuWeng Shao-PingSu Li-JenLai Tsung-Hsuan - Intervertebral disk pathology, including disk herniation and degeneration, is a major contributor to chronic low back pain, and when conservative treatment fails, surgical management often involves discectomy-based procedures that leave residual annulus fibrosus (AF) defects associated with reherniation and progressive degeneration. These limitations have motivated interest in regenerative strategies using biomaterial scaffolds; however, reproducing the hierarchical, angle-ply architecture of the AF remains challenging. Here, we present a single-step extrusion-based 3D-printing approach to fabricate polycaprolactone (PCL) scaffolds with aligned microscale surface grooves that promote AF-like organization. Patterned nozzles with circumferential peaks generated uniaxial concave microgrooves (10-17 µm wide) directly during printing, enabling formation of multilamellar angle-ply constructs. Human bone marrow-derived mesenchymal stem cells cultured on patterned scaffolds aligned longitudinally within concave grooves, forming end-to-end arrays that guided extracellular matrix deposition. Gene expression analysis showed that topographical cues governed cellular organization without significantly altering gene expression profiles, while TGF-β3 supplementation upregulated outer AF-associated markers, including COL1, COL12, SFRP2, MKX, MCAM, and SCX. TAGLN expression increased specifically on patterned scaffolds in the absence of TGF-β3, indicating an association between microgroove-guided cellular organization and TAGLN expression, warranting further investigation into potential tension-related mechanisms. This novel single-step extrusion-printing approach leverages custom nozzle geometry to impart concave microgrooves, facilitating scalable fabrication of multilamellar angle-ply scaffolds that induce aligned cellular organization and support potential applications in annulus fibrosus repair, as well as mechanobiological studies of anisotropic musculoskeletal tissues. - Source: PubMed
Publication date: 2026/03/11
Kluser NadineAlig Gion UrsinSprecher ChristophWoods XavierGrad SibylleAlini MauroHäckel SonjaAlbers Christoph EEglin DavidNarayanan RajkishenVernengo Andrea J