Ask about this productRelated genes to: CD117 antibody
- Gene:
- KIT NIH gene
- Name:
- KIT proto-oncogene, receptor tyrosine kinase
- Previous symbol:
- PBT
- Synonyms:
- CD117, SCFR, C-Kit
- Chromosome:
- 4q12
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: CD117 antibody
Related articles to: CD117 antibody
- Diabetic foot ulcer (DFU) is a severe complication of diabetes mellitus characterized by impaired wound healing and high amputation risk. Current treatments remain unsatisfactory, necessitating exploration of novel molecular mechanisms. The transcription factor CCAAT enhancer binding protein delta (CEBPD) regulates inflammatory responses and cellular stress pathways implicated in diabetic complications. However, its specific role and mechanism in DFU pathogenesis are poorly understood. Human umbilical vein endothelial cells (HUVECs) were exposed to high glucose (HG) to mimic diabetic conditions. C-X-C motif chemokine ligand 10 (CXCL10) and CEBPD mRNA expression were analyzed by quantitative real-time polymerase chain reaction. The protein expression of CXCL10, CEBPD, and glutathione peroxidase 4 (GPX4) was detected by western blotting assay. Cell viability was analyzed by a cell counting kit-8 assay. Cell proliferation was analyzed by a 5-ethynyl-2'-deoxyuridine assay. Cell apoptosis was detected by flow cytometry. Cell migration was analyzed by a wound-healing assay. Tube formation was analyzed by a tube formation assay. Fe and malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were analyzed by colorimetric assays. Reactive oxygen species (ROS) levels were analyzed by fluorometric assay and flow cytometry. The chromatin immunoprecipitation (ChIP) assay and dual-luciferase reporter assay were used to analyze the association of CXCL10 and CEBPD. The effect of CEBPD knockdown on HG-induced cell injury was further analyzed using a DFU rat model. CXCL10 and CEBPD expression were significantly upregulated in DFU patients and HG-stimulated HUVECs. CXCL10 knockdown reversed HG-induced cellular damage, restoring proliferation, migration, and tube formation while suppressing apoptosis and altering ferroptosis-related indicators (evidenced by reduced Fe/MDA/ROS and elevated SOD/GPX4). Crucially, CEBPD transcriptionally activated CXCL10, confirmed by promoter binding (ChIP) and luciferase activity. Silencing CEBPD replicated the protective effects of CXCL10 knockdown, and CXCL10 overexpression attenuated CEBPD knockdown-induced effects in HG-treated HUVECs. In vivo, CEBPD knockdown accelerated diabetic wound closure in rats, enhancing collagen deposition and tissue regeneration. CEBPD exacerbated human umbilical vein endothelial cell dysfunction at least partly through the transcriptional regulation of CXCL10, and CEBPD knockdown accelerated diabetic wound healing in vivo, suggesting that targeting the CEBPD/CXCL10 interplay may serve as a potential therapeutic strategy for DFU. - Source: PubMed
Publication date: 2026/05/04
Liang QiguoWang DexianXu HaiboYang JuanXu Qinglian - Genetic alterations represent key therapeutic targets in melanoma, but genomic profiles differ across ethnic groups and remain poorly defined in Thai patients. We characterized the somatic landscape of 33 melanoma samples (13 cutaneous, 13 acral, 7 mucosal) using the 501-gene OCA-Plus panel with next-generation sequencing, integrating clinical and genomic features. The somatic landscape revealed BRAF as the most frequently mutated gene, primarily involving the V600E variant, followed by KIT. Both BRAF and KIT emerged as significant drivers and exhibited mutual exclusivity, with BRAF mutations clustering specifically in cutaneous melanoma. Given the limited cohort size and heterogeneity, cross-ethnic comparisons of major mutational drivers revealed no statistically significant differences, necessitating further large-scale validation. Mutations in the RAS-MAPK and NOTCH oncogenic signaling pathways were the most frequent, and co-occurring alterations in MYC and Cell Cycle pathways were observed. Putative clinically actionable variants-primarily in BRAF, KIT, and NRAS-were present in 48.5% of patients, occurring most frequently in cutaneous melanoma. Notably, patients harboring KIT mutations showed a trend toward shorter disease-free survival, suggesting a potential prognostic role that warrants further investigation. These findings provide initial genomic understanding of Thai melanoma and highlight candidate actionable mutations for future precision oncology research. - Source: PubMed
Publication date: 2026/05/03
Kamdee KornyokPithukpakorn ManopSitthinamsuwan PanittaParingkarn TeerapatThumrongtharadol JanistaAchariyapota VuthinunTaweepraditpol SitthichokeChairatchaneeboon Manasmon - Dried blood spot (DBS) biosampling holds promise for expanding routine viral load (VL) monitoring for youth with HIV (YWH), particularly those at highest risk for HIV medication non-adherence. This mixed methods study piloted home-based DBS collection with YWH, aged 15-24 years. We enrolled 34 YWH with suppressed VL from April 22, 2020, to December 15, 2021, a subset of a fully virtual, nationwide decentralized clinical trial (ATN 144 SMART). Participants were mailed a HemaSpot-HF kit and asked to complete a computer-assisted self-interview (CASI) with an instructional DBS video. Surveys and semi-structured interviews provided quantitative and qualitative data to assess feasibility, appropriateness, and acceptability of home-based DBS for VL monitoring. Of 239 total screener attempts, 134 individuals were eligible and 115 provided contact information/completed the screener; 34 enrolled and returned DBS kits. Descriptive analyses showed a positive relationship between perceived suitability, feasibility, and acceptability. Perceived suitability was negatively associated with age, and feasibility differed significantly by health insurance coverage. Qualitative findings identified facilitators such as clinic/provider support, awareness of DBS innovation, insurance coverage, and streamlined mailing processes. Barriers included living environment challenges, cost concerns, and mail delivery issues. This pilot supports a self-management model and provides preliminary evidence that home-based DBS collection is feasible and acceptable among YWH. Scaling up this method through clinic and provider promotion could transform YWH HIV care by enabling remote VL monitoring. Findings also underscore the value of DBS as a practical biospecimen collection strategy for decentralized research models. - Source: PubMed
Publication date: 2026/05/03
Gurung SitajiMacDonell KarenSizemore K MarieFerraris Christopher MNaar Sylvie - Alkyne complexes are known for transition metals across the d-block with exception of the radioelement technetium despite considerable synthetic efforts. DFT calculations suggest that this is not inherent to the transition metal but a consequence of the overall ligand sphere. The arsenic-based tolane ligand 1,2-bis(2-(diisopropylarsaneyl)-4-(trifluoromethyl)phenyl)ethyne (L ) forces a coordination of the central alkyne moiety onto the metal through ligand design. The stable, crystalline Tc(III) and Tc(V) alkyne complexes mer-[TcCl(κ-As,CC,As-L )], mer-[TcNX(κ-As,CC,As-L )] (X = Cl, Br) and cis,trans,mer-[TcN(CN)Cl(κ-As, CC, As-L )] alongside their rhenium homologs mer-[ReCl(κ-As,CC,As-L )] and mer-[ReNCl(κ-As,CC,As-L )] have been prepared and fully characterized. According to spectroscopic and DFT analyses, the technetium complexes represent robust, classical 2e alkyne complexes, while a different situation was found for mer-[ReCl(κ-As,CC,As-L )] with a formally oxidized metal ion and reduced 4e donor ligand. This has general implications for π-ligand coordination in group 7 and potentially for neighboring elements. Successful translation to the medicinally relevant nuclear isomer Tc proves the viability of alkyne donors as building blocks for stable chelation of technetium at the tracer level. - Source: PubMed
Publication date: 2026/05/02
Roca Jungfer MaximilianErnst Moritz JohannesEberle LukasKesselring MariusClaude GuilhemBallmann Joachim - Malaria constitutes a major public health burden in Sudan, accounting for most outpatient visits and hospital admissions across approximately 80% of the states. The armed conflict beginning in April 2023 severely disrupted an already fragile health system, affecting the surveillance system infrastructure. No prior studies have assessed the impact of conflict on routine malaria surveillance data reported through District Health Information Software 2 (DHIS2). This study evaluated the effects of conflict on completeness and reporting of malaria impact indicators data across Sudanese states. - Source: PubMed
Publication date: 2026/05/02
Alnaeem Khlood FathiSaha Unnati RaniAhmed Hewida FathalrhmanMergenthaler Christina