Ask about this productRelated genes to: CD117 antibody
- Gene:
- KIT NIH gene
- Name:
- KIT proto-oncogene, receptor tyrosine kinase
- Previous symbol:
- PBT
- Synonyms:
- CD117, SCFR, C-Kit
- Chromosome:
- 4q12
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: CD117 antibody
Related articles to: CD117 antibody
- The liver fluke, Fasciola hepatica, is considered one of the principal issues affecting the health and productivity of the UK sheep population. Diagnostic testing is advised to ensure appropriate use of treatment. A new lateral flow test to detect antibodies to liver fluke has recently been developed for use by farmers. Point of care rapid diagnostic tests are rare in agriculture so this represents a novel innovation in this sector. - Source: PubMed
Publication date: 2026/09/29
Howell Alison KCwiklinski KrystynaWalsh Tessa RHiggins Helen M - von Meyenburg complex (VMC) is a rare ductal plate malformation, typically presenting as asymptomatic benign intrahepatic biliary microhamartomas. However, when secondarily infected, VMC can become the source of cryptic recurrent septicaemia, posing a substantial diagnostic challenge, particularly in patients with a known history of chronic source of comorbid infection. - Source: PubMed
Publication date: 2026/10/02
Ho Chi LaiChen SirongWong Yuet HungWu Kwan KitYip YuLin Wai ChiCheung Shing Kee - Lung squamous cell carcinoma (LUSC) is an aggressive malignancy with a poor prognosis. Given the association between mitochondrial dysregulation and LUSC, the aim of the present study was to identify mitochondrial unfolded protein response (UPR)‑related candidate genes associated with LUSC and to explore their potential functional relevance. In the present study, differential expression, univariate Cox and survival analyses were combined to identify a UPR‑associated key gene in LUSC. Gene set enrichment analysis was then performed, and overexpression (OE) and knockdown cell lines were used to clarify its function using western blotting, Cell Counting Kit‑8, colony formation and Transwell invasion assays. Using bioinformatics tools and a review of the literature, PTEN‑induced kinase 1 (PINK1) was singled out as a key gene in LUSC samples. Elevated PINK1 expression was associated with worse survival outcomes. A total of 117 pathways were enriched by PINK1, with the top 5 including 'focal adhesion' and 'mitogen‑activated protein kinase signaling pathway'. Using experimental analysis, it was observed that PINK1 significantly enhanced the proliferative, migratory, invasive and colony forming abilities of tumor cells, thereby exacerbating LUSC. Furthermore, these enhancements were diminished by the application of focal adhesion kinase (FAK) inhibitor. It was also noted that the expression levels of protein kinase B (AKT), p‑AKT, Ras homolog family member A (RhoA) and vinculin were all elevated in OE‑PINK1 cells. Collectively, these findings indicated that PINK1 exerted its influence on LUSC through the FAK/AKT/RhoA/vinculin pathway. The present study identified PINK1 as a key gene in LUSC, exerting its influence through the FAK/AKT/RhoA/vinculin pathway, thereby presenting a potential novel therapeutic target for the treatment of LUSC in the future. - Source: PubMed
Publication date: 2026/10/02
Li XuMa Yuhui - Cervical cancer is a malignant tumor that threatens women's health and radiotherapy represents a major therapeutic approach for its treatment; however, radioresistance frequently leads to treatment failure. The present study aimed to investigate whether loperamide (LOP), a µ‑opioid receptor agonist commonly used as an antidiarrheal agent, could enhance the radiosensitivity of cervical cancer cells by regulating ferroptosis. A Cell Counting Kit‑8 assay was used to evaluate the cytotoxicity of LOP in SiHa and HeLa cells, and EdU and colony formation assays were performed to assess cell proliferation. In addition, wound‑healing and Transwell assays were performed to detect cell migration. Flow cytometry was applied to measure cell apoptosis and intracellular reactive oxygen species (ROS) levels, and colorimetric assays were used to determine malondialdehyde (MDA) and glutathione (GSH) contents. The mRNA and protein expression levels of the ferroptosis‑related markers GSH peroxidase 4 (GPX4) and solute carrier family 7 member 11 (SLC7A11) were analyzed using reverse transcription‑quantitative PCR and western blotting. The results showed that LOP significantly inhibited the viability of SiHa and HeLa cells, and exerted a synergistic effect with radiotherapy (combination index <1) to suppress cell proliferation and migration. Combined treatment with LOP and radiotherapy markedly increased apoptosis and ROS accumulation, elevated MDA levels and reduced GSH levels. Moreover, the combination treatment significantly downregulated the expression of GPX4 and SLC7A11. These findings indicated that LOP enhances the radiosensitivity of cervical cancer cells, with ferroptosis induction via inhibition of the GPX4/SLC7A11 axis as a contributing mechanism. Both apoptosis and ferroptosis were induced by the combination treatment, suggesting that the radiosensitizing effect may involve multiple cell death pathways. These observations provide a basis for further investigation into LOP as a potential radiosensitizing agent in cervical cancer. - Source: PubMed
Publication date: 2026/10/02
Guo HuarongLi HaixiaTian ShunkangZhang YunkaiWu FuyunKe Jing - Cholesterol oxidase (CO, EC 1.1.3.6) is widely used in clinical diagnostics for the enzymatic determination of cholesterol in human serum and plasma. Among bacterial COs, the type II CO from (BCO), characterized by a covalently bound FAD cofactor, is particularly attractive due to its high stability and favorable kinetic properties. In this study, we developed an integrated bioprocess for recombinant BCO production in . Two His-tagged variants were constructed and compared: a C-terminally tagged form (BCO-H) and an N-terminally tagged form (H-BCO). Expression was optimized in a chemically defined modified M9 medium and scaled up using a fed-batch strategy. Both variants reached high cell densities (OD₆₀₀ ≈ 50) and showed a > 20-fold increase in volumetric productivity compared to shake-flask cultures. BCO-H outperformed H-BCO, reaching a final productivity of 102 ± 1.5 KU/L. Single-step Ni-affinity purification yielded highly pure enzymes with specific activities comparable to commercial CO. Both enzymes were successfully incorporated into a commercial cholesterol diagnostic kit formulation and evaluated according to European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) quality specifications. The in-house formulations showed excellent linearity, high precision, low bias, and total error within desirable limits, with comparable or faster kinetics than the reference formulation. Importantly, analytical performance was fully preserved after one year of storage at 4 °C. Overall, this work establishes a scalable fed-batch production process for recombinant type II CO suitable for use in real clinical diagnostic settings, achieving the highest reported volumetric productivity to date. - Source: PubMed
Publication date: 2026/09/09
Godino AgustinaAmaranto MarillaRassetto MauricioVila María SoledadAltamirano GabrielaSoria MagalíSalinas Silvina RosaBarra José Luis