Ask about this productRelated genes to: SOX17 antibody
- Gene:
- SOX17 NIH gene
- Name:
- SRY-box 17
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 8q11.23
- Locus Type:
- gene with protein product
- Date approved:
- 2002-02-15
- Date modifiied:
- 2015-11-23
Related products to: SOX17 antibody
Related articles to: SOX17 antibody
- DNA methylation might influence the expression of genes that regulate coronavirus disease 2019 (COVID-19) progression. This work explored the significance of DNA methylation of 7 genes (TAC1, CDO1, HOXA9, ZFP42, SOX17, RASSF1A, and SHOX2) in blood circulating free DNA (cfDNA) in differentiating COVID-19 infections and recoveries. The correlation with changes in the proportion of immune cell populations in the recovery period was analyzed. - Source: PubMed
Publication date: 2026/07/31
Xiao XiaoXu RenDu ChaoxiangYin JunXin BeibeiKe ZhongheLi XiyanZhang HaoChen Xinyu - Cholangiocarcinoma (CCA) is a biliary cancer with a poor prognosis and marked chemoresistance. The transcription factor SOX17, which is essential for cholangiocyte differentiation, is frequently downregulated in CCA, as is the case with several tumor suppressor genes. This study provides a proof-of-concept for further development of cellular therapeutic strategies to restore SOX17 expression in CCA cells. For this aim, we used EGI-1 cells as the target cell model derived from extrahepatic CCA. Recombinant Tat-SOX17 protein was first produced in bacteria and purified using Ni-NTA affinity columns and asymmetric flow-field-flow fractionation. Tat-SOX17 entered EGI-1 cells and reached their nucleus. The addition of the α-fetoprotein signal peptide (AFPsp) to the chimeric protein enabled the efficient secretion of the fusion protein formed by AFPsp, SOX17, and reverse Tat (Tat) by donor cells. For the production of secretome enriched in AFPsp-SOX17-Tat protein, HEK293T cells, derived from embryonic kidney, were used. In EGI-1 cells cultured in the presence of this secretome, target gene expression, cell cycle progression, apoptosis, proliferation, colony formation, and cell migration were assessed. Protein expression and localization were analyzed by Western blotting and immunofluorescence. AFPsp-SOX17-Tat enters EGI-1 cells, reaches their nucleus, and modulates the expression of SOX17-dependent genes, such as ABCB1 and ABCG2. Moreover, a marked reduction in proliferation and colony-forming ability was found. In contrast, no significant effect on cell cycle progression, apoptosis, or cell migration was observed. Similar treatment of immortalized human cholangiocytes also increased their SOX17 content, resulting in upregulation of the cholangiolar marker cytokeratin 7 (CK7), but did not affect their proliferation rate. In conclusion, using chimeric proteins such as AFPsp-SOX17-Tat, which contain components for secretion from donor cells and entry into target cancer cells, can provide a promising approach for treating tumors such as CCA, which are characterized by reduced expression of tumor suppressor genes, including SOX17. - Source: PubMed
Publication date: 2026/08/04
Peleteiro-Vigil AnaOrtiz-Rivero SaraIzquierdo-Mateo YaniraSanchez-Vicente LauraGonzález-Sánchez EsterVaquero JavierArevalo Juan CarlosBriz OscarMonte Maria JMarin Jose J GHerraez Elisa - This study explores a stem-cell-based approach for diabetes treatment by enhancing the viability and functionality of insulin-producing cells (IPCs) derived from stem cells of the apical papilla (SCAP). Although SCAP can differentiate into IPCs, limited cell survival remains a challenge. To address this, the proliferation enhancer WS6 was incorporated into Eudragit RS100 nanoparticles (NPs) using microfluidics. The WS6-loaded NPs were characterized for size, charge, PDI, morphology, stability, and drug loading. An MTT assay was performed as a preliminary screening method to evaluate the cytocompatibility of blank-NPs and to optimize treatment concentration. SCAP cells were treated with free WS6 or WS6-loaded NPs, and cellular uptake of NPs was evaluated using flow cytometry and fluorescence imaging. Additionally, the viability of treated cells was determined by propidium iodide (PI) and trypan blue. Prior to differentiation, definitive endoderm formation was assessed through SOX17 and FOXA2 expressions. After differentiation into IPCs, maturation markers such as insulin, C-peptide, PDX-1, NKX2.2, and NKX6.1 were examined, and apoptosis assays measured cell viability. Functional insulin secretion was tested using an in vitro glucose-stimulated insulin secretion (GSIS) assay. Results showed that WS6-loaded NPs significantly improved SCAP viability, increased healthy cell percentages, and enhanced IPC maturation. Treated IPCs demonstrated functional insulin secretion and improved glucose regulation. Overall, WS6-loaded NPs represent a promising approach to enhance IPC proliferation and generation for diabetes therapy. - Source: PubMed
Publication date: 2026/08/04
Abuarqoub DuaaMohammad MarwaAlbarghouthi RandAbuoun MohammadAlnatour MohammadAlhawarat FuadJaradat Abdolelah - - Source: PubMed
Publication date: 2026/08/03
Rojas AldanaMorales David L SWikenheiser-Brokamp Kathryn AHayes Don - The cerebral cortex is highly sensitive to oxygen availability and thus serves as an ideal model for studying neural adaptation to hypoxia. Here, we integrated long-read (Oxford Nanopore) and short-read (Illumina) single-cell RNA sequencing to systematically characterize the cellular composition and transcriptional landscape of the cerebral cortex in high-altitude Diqing Tibetan pigs (DT, ~3200 m) and low-altitude Diannan small-ear pigs (DSE, ~500 m). Single-cell analysis identified nine major cortical cell types and revealed pronounced transcriptional divergence between the breeds. In DT, multiple genes associated with high-altitude hypoxia adaptation and energy homeostasis, including NKAIN2, CTNNA3, ZFP36, and HSP40 family members, were significantly upregulated. Functional enrichment highlighted mitochondrial oxidative phosphorylation and energy metabolism pathways, suggesting metabolic reprogramming under chronic hypoxia. Key transcription factors, including SOX17, NKX6-2, ETV4, and SPI1, displayed cell-type-specific activity in Endo, Oligo, and Micro. Cell-cell communication analysis showed DSE with broader intercellular connectivity, whereas DT exhibited more focused and stronger signaling interactions. Integration of long-read sequencing further identified numerous novel, cell-type-specific transcripts, expanding porcine cortical transcriptome annotation. Collectively, these findings provide a comprehensive framework for understanding cellular and molecular adaptations of the cerebral cortex to prolonged high-altitude hypoxia. - Source: PubMed
Publication date: 2026/07/21
Chang YongchengDuan BofangHuo HailongZhang XiaLin WanWen FeidiGuo YimingWu LingxiangZou FengcaiHuo JinlongZhao Guiying