Ask about this productRelated genes to: NAPEPLD antibody
- Gene:
- NAPEPLD NIH gene
- Name:
- N-acyl phosphatidylethanolamine phospholipase D
- Previous symbol:
- -
- Synonyms:
- FMP30, C7orf18, NAPE-PLD
- Chromosome:
- 7q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2008-01-10
- Date modifiied:
- 2014-11-19
Related products to: NAPEPLD antibody
Related articles to: NAPEPLD antibody
- Post-traumatic headache (PTH) is a debilitating neurological sequela of mild traumatic brain injury (mTBI) characterized by secondary cephalic pain. The endocannabinoid system (ECS) is a critical modulator of nociception, yet the specific spatiotemporal changes in its metabolic machinery within cephalic pain circuits following mTBI are poorly understood. - Source: PubMed
Publication date: 2026/04/11
Nagarajan GurueswarZhang Yumin - Aluminum has been recognized as a reproductive toxin, yet the precise mechanisms by which embryonic exposure impairs male offspring development remain largely unclear. The key point is that no systematic adverse outcome pathway (AOP) network has yet been established to link aluminum exposure with reproductive disorders. This study employed an integrated approach-combining network toxicology and non-targeted metabolomics, and in vivo/in vitro experiments-to establish a novel AOP elucidating how aluminum induces spermatogenesis impairment in mice. Mechanistically, in vitro studies using mouse TM3 and primary Leydig cells demonstrated that aluminum chloride (AlCl₃) triggers oxidative stress, leading to endoplasmic reticulum stress (ERS), via the IRE1α/XBP1s pathway. We identified that the transcription factor XBP1s directly binds to and transactivates the promoter of N-acylphosphatidyl-ethanolamine-specific phospholipase D (Nape-pld). This cascade results in overactivation of the cannabinoid receptor 1 (CB1) and the downregulation of key steroidogenic proteins, Luteinizing Hormone Receptor (LHR) and Steroidogenic Acute Regulatory Protein (StAR). These findings provide compelling evidence for our AOP model, wherein aluminum induces ERS via oxidative stress, subsequently impairing testosterone synthesis through the endocannabinoid system (ECS). Our research advances the assessment of heavy metal reproductive risks by offering a robust methodology for identifying key events (KEs) and constructing causal AOP networks. - Source: PubMed
Publication date: 2026/03/24
Chen JunhanXia YunhuiRen KeYang FenglianLin ZongyuGan WeidongWang JunliLi Dongmei - Autosomal dominant polycystic kidney disease (ADPKD) is characterized by progressive cyst formation, inflammation, and metabolic dysregulation. The endocannabinoid system (ECS), particularly the cannabinoid-1 receptor (CBR), regulates renal metabolism and inflammatory signaling, yet its role in ADPKD remains largely unexplored. - Source: PubMed
Publication date: 2026/03/16
Betkar ShridharNemirovski AlinaRuppo ShmuelHinden LiadTam Joseph - Antidepressant drugs are the first-line treatment for chronic stress-related psychiatric disorders such as major depressive disorder, anxiety disorders, and post-traumatic stress disorder. However, their delayed-onset of therapeutic action, side effects, and incomplete clinical efficacy impose challenges for clinicians and patients' adherence to treatment. Cannabidiol (CBD) is a major non-psychotomimetic phytocannabinoid with a wide range of potential clinical applications such as either a standalone drug or as an add-on treatment. In our study, we found that in chronically stressed male mice, CBD (30 mg/kg) rapidly induced behavioral improvement within 7 days, which was quicker than the high dose of escitalopram (ESC, 14 days). Additionally, repeated administration of low and initially ineffective dose of CBD (7.5 mg/kg) potentiated the anti-stress effects of ESC (10 mg/kg) in mice subjected to 10 or 21 days of chronic unpredictable stress (CUS). Furthermore, our results suggested the involvement of N-acyl phosphatidylethanolamine phospholipase (NAPE-PLD) located in the prefrontal cortex (PFC) in the anti-stress effects of the 7-day treatment with ESC + CBD. This combination restored CUS-induced decreased expression of NAPE-PLD in the PFC. The behavioral effects of ESC + CBD were not observed in either constitutive NAPE-PLD knockout (KO) mice or mice with a CRISPR/Cas9-induced deletion of NAPE-PLD in the PFC. ESC + CBD treatment facilitated NAPE-PLD expression in parvalbumin (PV) interneurons in the PFC. As a conclusion, we suggest that CBD might be useful as an add-on therapy to optimize the action of (SSRI-)antidepressants, possibly by restoring the inhibitory/excitatory balance of the PFC via NAPE-PLD-mediated signaling. - Source: PubMed
Publication date: 2026/03/04
Scarante FrancieleFLopes Vinícius DFusse Eduardo JVicente Maria Ade Abreu Gabriel H DNardini VivianiSorgi Carlos ALirio Pedro H CGuo FengLu Hui-ChenHallak Jaime E CCrippa Jose AFaccioli Lucia HSales Katiuchia UGuimarães Francisco SMackie KenCampos Alline C - The endocannabinoid system (ECS) is involved in regulating immune functions in leukocytes. An inflammatory stimulus, specifically LPS, can modulate the activity of endocannabinoids (eCB) receptors, and eCB within the leukocytes can further exert either pro- or anti-inflammatory effects on the immune function of these cells. The effects of exogenous eCB on the cellular inflammatory responses of bovine leukocytes are largely unexplored; therefore, we aimed to evaluate the effects of incubation with the main eCB -arachidonoylethanolamide (AEA/anandamide) or 2-arachidonyglycerol (2-AG) on gene expression of eCB and inflammatory mediators following an ex vivo LPS challenge in dairy cow leukocytes. To this end, whole blood from mid-lactation dairy cows (n = 6) were subjected to ex vivo incubation with eCB (control [CTL], AEA at 0.29 µ, or 2-AG at 0.26 µ) for 2 h, followed by stimulation with or without LPS (10 ng/mL) for an additional 2 h. Overall, there were 6 treatments for cells from each cow: CTL, AEA, and 2-AG without LPS stimulation, and CTL+LPS, AEA+LPS, and 2-AG+LPS. Subsequently, RNA was extracted from leukocytes and assayed for gene expression levels via real-time quantitative PCR. First, we examined the main effects of LPS stimulation across eCB treatments: LPS decreased expression of the eCB receptors cannabinoid receptor 2 (), G protein-coupled receptor 55 (), and peroxisome proliferator-activated receptor gamma (). Furthermore, LPS increased expression of the eCB enzymes N-acyl phosphatidylethanolamine phospholipase D () and monoglyceride lipase () while reducing the expression of diacylglycerol lipase B () compared with non-LPS-stimulated groups. Then, we examined the main effects of eCB treatments on gene expression: incubation with AEA increased expression of cannabinoid receptor 1 () in leukocytes, whereas 2-AG increased the expression of the and tended to increase . In addition, 2-AG increased the expression of fatty acid amide hydrolase () and tended to increase the expression of . As expected, LPS increased the expression of inflammatory markers; however, incubation with eCB had no discernible effects on these genes. Taken together, ex vivo exposure of dairy cow leukocytes to AEA or 2-AG, with or without stimulation with LPS, resulted in differential effects on the expression of eCB receptors and enzymes, but we did not detect effects of exogenous eCB on the expression of inflammatory genes following an LPS challenge. The findings of the present study provide the first reductionist step in understanding the relationship between the ECS and inflammatory responses in immune cells of dairy cows. The complexity of the regulation of immune function in leukocytes, and its potential interplay with eCB, requires further studies to comprehensively elucidate the cellular mechanisms underlying these responses. - Source: PubMed
Publication date: 2025/12/13
Dos Santos Silva PButenko YHubner LShattenstein BZachut M