Ask about this productRelated genes to: MGLL antibody
- Gene:
- MGLL NIH gene
- Name:
- monoglyceride lipase
- Previous symbol:
- -
- Synonyms:
- HU-K5, MGL
- Chromosome:
- 3q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 2001-12-13
- Date modifiied:
- 2016-10-05
Related products to: MGLL antibody
Related articles to: MGLL antibody
- Monoacylglycerol lipase (MGLL) is a key serine hydrolase that regulates 2-arachidonoylglycerol (2-AG) and eicosanoid signaling. Inhibition of MGLL blocks the conversion of 2-AG into arachidonic acid (AA) with broad therapeutic implications in inflammation, cancer, and viral infection. Carbamate/urea inhibitors such as MJN110, JZL184, and SAR629 are widely used to irreversibly inhibit MGLL through covalent modification of the catalytic serine residue. Here, we demonstrate that this inhibitor class also induces proteasome-dependent degradation of MGLL, functioning as monovalent degraders. We show that loss of MGLL following covalent inhibitor treatment is dependent on the 26S proteasome, while detailed simulations of MGLL dynamics following inhibitor binding suggest that these inhibitors do not destabilize their protein target but instead induce conformational changes that likely facilitate polyubiquitination by exposing two lysine residues. Taken together, these findings establish carbamate/urea inhibitors as dual functional molecules with the propensity to both covalently inhibit their target and act as structural degraders, potentially functioning as monovalent molecular glues, highlighting the need to evaluate the degradation potential alongside inhibitory potency in future screening and drug discovery. - Source: PubMed
Publication date: 2026/05/07
Pham Jordan AThaingtamtanha ThanawatMcLeish WilliamLefebvre DavidUguccioni Spencer MFilip RoxanaGentile FrancescoPezacki John Paul - Lipophagy, a selective form of macroautophagy/autophagy, degrades lipid droplets (LDs) to provide energy and is implicated in metabolic disorders. The molecular mechanism underlying lipophagy induction remains incompletely understood. This study explored the role of SETDB1 in starvation-induced autophagy and lipophagy. We demonstrate that SETDB1 deficiency exacerbates starvation-induced hepatic lipid accumulation by inhibiting lipophagy. Mechanistically, starvation promotes ATM-mediated phosphorylation of SETDB1, which enhances its interaction with and methylation of the RNA helicase DDX5. In -knockout hepatocytes, hypomethylation of DDX5 facilitates the formation of the DDX5-METTL3-METTL14 complex, increasing mA modification of and mRNAs. This modification promoted YTHDF2-mediated decay of these transcripts, thereby inhibiting starvation-induced autophagy and lipophagy. Furthermore, administration of the SETDB1 activator -59 significantly enhances lipophagy and attenuates starvation-induced hepatic steatosis. Collectively, our findings reveal a novel pathway in which SETDB1 deficiency drives mA-mediated mRNA degradation to suppress lipophagy, thereby contributing to hepatic steatosis. AA free: amino acid deprivation; ATG14: autophagy related 14; ATG5: autophagy related 5; ATG7: autophagy related 7; ATM: ATM serine/threonine kinase; Baf A1: bafilomycin A; DDX5: DEAD-box helicase 5; FASN: fatty acid synthase; LAMP1: lysosome associated membrane protein 1; LAMP2A: lysosome associated membrane protein 2A; LIPE/HSL: lipase E, hormone sensitive type; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MAP3K5/ASK1: mitogen-activated protein kinase kinase kinase 5; METTL3: methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit; METTL14: methyltransferase 14, N6-adenosine-methyltransferase non-catalytic subunit; MGLL/MGL: monoglyceride lipase; OA: oleic acid; OSBPL8/ORP8: oxysterol binding protein like 8; PLIN2: perilipin 2; PNPLA2/ATGL: patatin like domain 2, triacylglycerol lipase; SETDB1: SET domain bifurcated histone lysine methyltransferase 1; TFEB: transcription factor EB; TP53/p53: tumor protein p53; ULK1: unc-51 like autophagy activating kinase 1; YTHDF2: YTH N6-methyladenosine RNA binding protein F2. - Source: PubMed
Publication date: 2026/05/17
Wang WenjunWang YanhongHou LigeWei XiaoyunGuo WenqiHuang JuanTan JunyangLu QiuxiaZhao QiJu ZhenyuLi JianshuangZhou Qinghua - The differences fatty acids and amino acids composition may be key factors influencing flavor variation among duck breeds. This study systematically analyzed the fatty acids and amino acids compositions in the pectoral muscle of 49 days old Cherry valley ducks (CV), Jinling white ducks (JL), and Liancheng white ducks (LC). Fatty acid analysis identified 27 fatty acids that exhibited significant differences across breeds (P < 0.05). The CV group had the highest total saturated fatty acid (SFAs) content, while total monounsaturated fatty acid (MUFAs) content ranked in the order of LC > JL > CV (P < 0.05). The total polyunsaturated fatty acids (PUFAs) content was significantly higher in both the JL and LC groups than in the CV group (P < 0.05), while the LC group showed a higher content than the JL group, though the difference was not significant. Amino acid analysis identified 20 amino acids that showed significant differences among breeds (P < 0.05). Furthermore, the total essential amino acids (EAAs) and non-essential amino acids (NEAAs) content ranked in the order of LC > JL > CV (P < 0.05). Whole-genome resequencing identified 2734 selected genes. The joint pathway analysis revealed co-enrichment of 9 fatty acids and 110 selected genes across 25 KEGG pathways, as well as 20 amino acids and 266 selected genes co-enriched in 78 KEGG pathways. RNA sequencing revealed 2539 differentially expressed genes (DEGs) between JL and CV, 6574 DEGs between JL and LC, and 7072 DEGs between LC and CV. Venn analysis showed that 9 and 24 of these DEGs overlapped with selected genes associated with fatty acid and amino acid variation, respectively. Further analysis revealed that the DEGs MGLL, NDUFV1, NDUFS4, and GNG10 may cooperatively regulate retrograde endocannabinoid signaling, thereby jointly influencing the deposition levels of arachidonic acid, γ-Aminobutyric-Acid, and l-glutamate in duck pectoral muscle. These findings provide a theoretical basis for the genetic improvement of meat quality traits. - Source: PubMed
Publication date: 2026/05/02
Xie HengliHu XinyueWang ShangminLiu HeheHe ZongliangJi HongjieLv KunpengZhu TingWang Jiwen - Post-traumatic headache (PTH) is a debilitating neurological sequela of mild traumatic brain injury (mTBI) characterized by secondary cephalic pain. The endocannabinoid system (ECS) is a critical modulator of nociception, yet the specific spatiotemporal changes in its metabolic machinery within cephalic pain circuits following mTBI are poorly understood. - Source: PubMed
Publication date: 2026/04/11
Nagarajan GurueswarZhang Yumin - Glyphosate, one of the most widely used herbicides worldwide, has raised significant concerns regarding its potential involvement in hepatotoxicity and molecular changes associated with liver cancer biology. These concerns highlight the need to better understand its underlying molecular mechanisms in hepatoma cells. Emerging evidence suggests that glyphosate exposure may increase the risk of liver cancer and chronic liver disease. However, the precise molecular alterations and promising biomarkers associated with glyphosate-induced hepatic toxicity and disease remain largely unexplored. In this study, an RNA-Seq-based in silico systems biology approach was employed to elucidate glyphosate-induced differential transcriptional profiling in hepatoma cells. This analysis revealed significant transcriptional profiling characterized by the upregulated hub genes and . These genes were primarily associated with glucose metabolism, TNF-α/NF-κB signaling, epithelial-mesenchymal transition (EMT) and cellular stress responses. Conversely, several key genes were significantly downregulated, including , , , , , , , , and , which were involved in lipid metabolism, immune regulation and non-alcoholic fatty liver disease (NAFLD) pathways. Notably, all hub genes demonstrated strong diagnostic performance, highlighting their potential as sensitive biomarkers of glyphosate exposure. Collectively, this study provides comprehensive insights into gene expression changes associated with glyphosate exposure in hepatoma cells, linking them to hepatic metabolic dysregulation and immune modulation and suggesting a panel of hub genes with potential diagnostic and therapeutic significance. - Source: PubMed
Publication date: 2026/03/19
Mishra Divya