Ask about this productRelated genes to: CD80 antibody
- Gene:
- CD80 NIH gene
- Name:
- CD80 molecule
- Previous symbol:
- CD28LG, CD28LG1
- Synonyms:
- B7.1, B7-1
- Chromosome:
- 3q13.33
- Locus Type:
- gene with protein product
- Date approved:
- 1993-12-14
- Date modifiied:
- 2016-10-05
Related products to: CD80 antibody
Related articles to: CD80 antibody
- This study aims to investigate the impact and molecular mechanism of ELAVL1 in promoting macrophage M1 polarization in Crohn's disease (CD) by upregulating S100A8 expression. - Source: PubMed
Publication date: 2026/05/03
Jia LinChen YingtaiLu Ying - : This study aimed to investigated the role of Giardia extracellular vesicles (EVs) in intercellular communication and to evaluated their potential as vaccine candidates. The immunomodulatory effects of Giardia EVs were assessed in mouse macrophages and human monocyte-derived dendritic cells (Mo-DCs), with a particular focus on key inflammatory signaling pathways. In vivo immunogenicity was evaluated following EV administration, and the antigenic composition of EV cargo was characterized by proteomic analysis. Giardia EVs activated pro-inflammatory signaling pathways in mouse macrphages, including SAPK/JNK, ERK1/2, and NF-κB. This activation was associated with IκB-α degradation and nuclear translocation of p65. Furthermore, EV stimulation significantly upregulated the expression of pro-inflammatory genes, including Il1β, Il6, Il4, Ptgs2, Nos2, and Tnf, with log fold changes ranging from 3.9 to 15.8. Consistently, EVs increased iNOS protein expression (28-45%) and nitrite production (9.6-12.3-fold). In human Mo-DCs, Giardia EVs promoted cellular maturation, as evidenced by increased expression of MHC-II, CD80, and CD86, and enhanced T-cell proliferation with a Th1-skewed profile. In vivo immunization induced antigen-specific antibody responses, with IgG subclass distribution indicative of a balanced Th1/Th2 response. Proteomic analysis identified immunoreactive EV-associated proteins, including elongation factor 1-alpha, α-7.3 giardin, tubulin, and variant surface proteins (VSPs), which are well-established antigens in Giardia infection, with prominent bands observed at approximately 22 kDa and 50 kDa. Collectively, these findings demonstrate that Giardia EVs modulate innate immune responses in vitro, elicit antigen-specific humoral immunity in vivo, and contain conserved immunogenic proteins. These properties support their potential as a promising cell-free vaccine platform against giardiasis. - Source: PubMed
Publication date: 2026/04/09
Faria ClarissaJesus SandraFerreira BárbaraLourenço ÁgataSebastião Ana IsabelMateus DanielaNeves Bruno MBorges OlgaCruz Maria TeresaSousa Maria do Céu - To establish a foundation for conserving and utilizing local frog germplasm resources in Zhejiang Province, for which has high commercial and nutritional value, a pan-genome analysis was performed. - Source: PubMed
Publication date: 2026/03/31
Zhu Zhi-HuiShu Miao-An - For in vitro studies involving macrophages, an efficient protocol for isolation and cell culture of monocyte-derived macrophages (MDM) is essential. However, it remains unclear how far isolation steps improve purity or provoke cell changes. Here we compare the characteristics and functionality of macrophages isolated from leukocyte reduction (LRS) chambers by either CD14+ magnetic sorting (sMac) or by seeding PBMC with separation by plastic adhesion and subsequent washing steps (uMac) to evaluate the necessity of magnetic sorting. - Source: PubMed
Publication date: 2026/05/01
Thomé JuliaZeller JohannesChristmann ThierryBogner BalazsLind MaikeDreck MarieBrose Teresa ZSchneider LauraPankratz FranziskaKreuzaler SheenaEisenhardt Steffen U - Human breast milk (HBM) is an ideal nutritional source for the growth and development of infants. In addition, HBM contains hormones, growth factors, microRNAs and exosomes that perform various physiological functions. This study investigates the immunomodulatory effects of HBM-derived exosomes on myeloid cells and chondrocytes, and implications for juvenile idiopathic arthritis. HBM-derived exosomes were isolated and characterized using nanoparticle track analyzer and Western blotting. The HBM-derived exosomes treatment decreased the expression of inflammatory mediators and proinflammatory cytokines in mouse peritoneal macrophages upon lipopolysaccharide stimulation. Flow cytometry analysis of bone marrow-derived macrophages indicated that exosomes promoted M2 polarization, as evidenced by a decrease in cells expressing CD80 (M1 marker) and a concurrent increase in cells expressing M2 marker CD206. In addition, exosome treatment attenuated the mitogen-activated protein kinase signaling pathway by reducing the phosphorylation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38 mitogen-activated protein kinase, and IκB-α, thereby reducing the expression of inducible nitric oxide synthase, cyclooxygenase-2, metalloprotease (MMP)-1, MMP-3, and MMP-13 in SW1353 chondrocytes following IL-1β stimulation. These findings suggest that HBM-derived exosomes promote macrophage polarization toward an anti-inflammatory M2 phenotype and exert significant immunomodulatory effects. - Source: PubMed
Publication date: 2026/04/30
Kwon DahyeonYoo Ji YoonDan Kang-BinKim Ki-UkLee Ji-YunMin Hyeyoung