Ask about this productRelated genes to: BCHE antibody
- Gene:
- BCHE NIH gene
- Name:
- butyrylcholinesterase
- Previous symbol:
- CHE1, CHE2
- Synonyms:
- E1
- Chromosome:
- 3q26.1
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2016-10-05
Related products to: BCHE antibody
Related articles to: BCHE antibody
- Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder that requires therapeutic agents capable of targeting multiple pathological pathways. In this study, a series of cannabidiol (CBD)-like hydrazone derivatives (-) was synthesized and characterized by NMR, HRMS, and single-crystal X-ray diffraction for compound . In silico ADME analysis predicted favorable drug-like properties, including compliance with Lipinski's Rule of Five, oral bioavailability, and blood-brain barrier permeability. The compounds were evaluated for cholinesterase inhibition, antioxidant activity, cytotoxicity in neuronal cell lines, and binding interactions with human butyrylcholinesterase (hBChE) by molecular docking. Biological evaluation revealed a marked preference for BChE over acetylcholinesterase (AChE). Compound was the most potent BChE inhibitor (IC = 1.67 ± 0.11 μM), while compounds and demonstrated high selectivity toward BChE. Antioxidant assays (DPPH, ABTS, FRAP, and FTC) indicated moderate, mechanism-dependent activity. Compounds and showed the strongest ABTS radical-scavenging effects, whereas compounds and provided the greatest protection against lipid peroxidation, surpassing CBD under the tested conditions. Several derivatives, particularly , , , , and , exhibited favorable safety profiles in SH-SY5Y and Neuro-2a cells. Molecular docking supported the experimental findings. Overall, compounds and emerged as promising multifunctional leads for the development of multitarget-directed anti-Alzheimer agents. - Source: PubMed
Publication date: 2026/07/30
Stoyanov Boris PGeorgiev BorislavStefanova DenitsaTzankova VirginiaKalcheva-Yovkova ElenaVassilev NikolayRangelov MiroslavTodorova NadezhdaZheleva-Dimitrova DimitrinaShivachev BorisAngelova Violina T - The present paper focuses on the analyses of enzyme Inhibition and antioxidant properties of acetone, ethanol and water extracts of Melissa officinalis L. The biologically active molecular components of the mentioned extracts of the plant were reported with the help of through validated LC-MS/MS method. Validated LC-ESI-MS/MS analysis identified quinic acid, protocatechuic acid, protocatechuic aldehyde, vanillin, p-coumaric acid, naringenin, luteolin, apigenin, kaempferol, hesperetin, and acacetin as representative phytochemicals exhibiting distinct solvent-dependent distribution patterns. To test the antioxidant capacity of the extracts, several popular antioxidant capacity determination methods were used, including DPPH, ABTS, CUPRAC, and FRAP assays. The acetone extract exhibited the strongest antioxidant activity, showing DPPH, ABTS, CUPRAC, and FRAP values of 195.10, 246.39, 467.09, and 266.96 mg TE/g, respectively. Enzyme inhibition studies demonstrated that the ethanol extract showed the highest inhibitory activity against AChE (2.88 mg GALAE/g), BChE (1.92 mg GALAE/g), tyrosinase (55.90 mg KAE/g), α-amylase (0.18 mmol ACAE/g), and α-glucosidase (3.35 mmol ACAE/g). In the theoretical part of the study, chemical reactivity analyses about the major molecular components in the extracts were done using Density Functional Theory (DFT) based parameters and electronic structure principles. In addition, the strength and mechanisms of interaction of these molecules with the enzymes studied in the experimental part were tested through molecular docking and molecular dynamics simulations. Experimental and computational analyses were supported each other. - Source: PubMed
Publication date: 2026/08/02
Saraç HandanBuluz Emre CanDemirbaş AhmetZengin GökhanAbdullah Yilmaz MustafaKaya Savaş - Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cholinergic dysfunction, making acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) attractive therapeutic targets. In the present study, twelve novel phenolic Mannich base-derived hydrazones incorporating a diaryl ether scaffold were rationally designed, synthesized, and evaluated as cholinesterase inhibitors. All synthesized compounds exhibited potent nanomolar inhibition against both cholinesterases, with K values ranging from 24.27 to 98.56 nM for AChE and 28.68-148.41 nM for BChE. Compound 5 was identified as the most potent AChE inhibitor (K = 24.27 nM), whereas compound 6 showed the highest BChE inhibitory activity (K = 28.68 nM). Both compounds were considerably more potent than the reference inhibitors tacrine and donepezil. Enzyme kinetic studies revealed that all compounds acted as competitive inhibitors. Cytotoxicity evaluation against SH-SY5Y neuronal cells demonstrated low toxicity, with IC values ranging from 33.41 to 46.79 μM, indicating that effective cholinesterase inhibition occurred at concentrations far below those affecting neuronal cell viability. Molecular docking and MD simulations demonstrated stable binding of the most active compounds within the catalytic gorge of both enzymes through persistent hydrogen bonds, cation-π, and π-π interactions with key active-site residues. MM-GBSA and energy decomposition analyses further supported their favorable binding affinities. In addition, the synthesized compounds exhibited acceptable predicted pharmacokinetic properties, including favorable parameters related to blood-brain barrier permeability and oral absorption. These results identify phenolic Mannich base-derived hydrazones as promising lead compounds for the development of new cholinesterase inhibitors for the treatment of Alzheimer's disease. - Source: PubMed
Publication date: 2026/08/07
Şenol HalilDemir YelizTokalı PelinŞenol Ayşe MerveAkyıldırım OnurTokalı Feyzi Sinan - The investigation of the biological activities of plants utilized in traditional medicine to treat various illnesses is of great importance for their potential evaluation as pharmaceutical raw materials in modern medicine. In the present study, the anticancer, antimicrobial, and anti-quorum-sensing (anti-QS) activities of ethyl acetate, ethanol, ethanol-water, and water extracts prepared from the aerial parts of by maceration were tested. The biological content, antioxidant, and enzyme-inhibitory capacity of the extracts were also investigated. extracts possess a rich flavonoid and phenolic composition. The ethanol-water extract had the highest total polyphenol content. 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, vanillic acid, p-coumaric acid, and ferulic acid were common and abundant across all extracts. The ethanol extract showed the highest enzyme-inhibitory activity in acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) tests with values of 2.68 and 3.17 mg GALAE/g, respectively. The anticancer activity results revealed that the lowest 50% inhibitory concentration (IC) for cell viability in the MDA-MB-231 cell line was 215.2 μg/mL for the ethanol extract. For the HeLa cell line, the lowest IC value was defined as 151.7 μg/mL for the ethyl acetate extract. No antimicrobial effects were detected in the tested concentrations of the extracts against reference microbial strains. To determine anti-QS activity, the inhibitory effect on violacein pigment production was investigated on the CV026 mutant strain. No inhibition of pigment production was observed in the study. Based on the findings, may have potential for further anticancer research and pharmacological studies. - Source: PubMed
Publication date: 2026/08/10
Seyhan KübraAkıllı GamzeBaloglu PınarKanat Ayşenur ÖzdemirIscen Nurdan TugbaCaprioli GiovanniSantanatoglia AgneseRicciutelli MassimoBaloglu Mehmet CengizAltunoglu Yasemin CelikBahsi MuammerTerzic MilenaZengin Gökhan - The sub-lethal effects of plastics and their associated chemicals on wildlife are poorly understood, primarily due to the lack of reliable indicators. We used southern giant petrels (Macronectes giganteus, SGP), key scavengers and predators in southern seas, to assess whether early-life exposure to plastics was associated with health parameters. We examined relationships between plastic ingestion (≥ 1 mm) and biomarkers of enzymatic activities (AChE acetylcholinesterase, BChE butyrylcholinesterase and GST glutathione S-transferase) and conventional health parameters (haematological, biochemical and morphometric data) in SGP chicks from colonies in Chubut, Argentina. During 2022 and 2023, plastics were detected in 71.4% of chicks (1-14 items, mean mass = 0.2510 g, representing < 0.1% of chick body mass). In 30-day-old chicks, plastic ingestion was associated with nutritional parameters (cholesterol, total solids, packed cell volume, calcium, body mass and body condition index), metabolism (enzymes aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatinine phosphokinase) and immune response (eosinophil percentage). Associations with biomarkers (AChE, BChE, and GST) were observed but lost significance when accounting for inter-annual variation. By fledging (∼90 days), most relationships weakened or disappeared, while GST activity and calcium levels emerged as the only variables associated with plastic ingestion. It is unclear whether these associations reflect compensatory physiological responses to oxidative challenge, contaminant exposure, or developmental/ontogenetic adjustments. Our results highlight the value of multi-indicator approaches for detecting subtle physiological variation associated with plastic ingestion. Importantly, the pervasive threat of plastic pollution warrants investigating whether early-life exposure influences post-fledging survival and fitness. - Source: PubMed
Publication date: 2026/08/11
Gallo LucianaQuintana FlavioAttademo Andrés MUhart Marcela M