Ask about this productRelated genes to: BCHE antibody
- Gene:
- BCHE NIH gene
- Name:
- butyrylcholinesterase
- Previous symbol:
- CHE1, CHE2
- Synonyms:
- E1
- Chromosome:
- 3q26.1
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2016-10-05
Related products to: BCHE antibody
Related articles to: BCHE antibody
- This study investigated the molecular and histopathological mechanism(s) contributing to fluoride induced neurotoxicity in male and female rats, focusing on dysregulation of cholinergic, neurotrophic, glutamatergic pathways and the accompanying structural alterations in the brain tissue. Forty male and female rats were administered sodium fluoride in water at concentrations (ppm) of 0.5 (control), 50 (low), 150 (medium), and 300 (high dose) for 90 days. The mRNA expression levels of cholinergic markers (acetylcholinesterase-AChE, butyrylcholinesterase-BChE), the brain derived neurotrophic factor (BDNF) and the metabotropic glutamate receptor 5 (mGluR5) were quantified using RT-qPCR, and histopathological alterations of different regions of brain was assessed by hematoxylin and eosin (H&E) staining. Chronic NaF exposure resulted dose-dependent neurodegenerative changes in the hippocampal CA1, CA3, and dentate gyrus (DG) regions, characterized by neuronal shrinkage, pyknosis, vacuolization, and spongiosis. The histopathological lesions were more pronounced in male rats than in females. Chronic fluoride exposure also resulted in a dose-dependent decrease of blood AChE (1.09-0.604 mU/mL) and BChE (0.575-0.311 mU/mL) activities in both genders. Furthermore, chronic NaF exposure significantly down-regulated the expression of AChE, BDNF, and mGluR5 in fluoride-treated groups compared to controls. These findings demonstrate that prolonged NaF exposure disrupts cholinergic, neurotrophic, and glutamatergic signaling pathways, thereby contributing to neurotoxicity and potential neurodegeneration. Collectively, this mechanistic evidence underlines the importance of public health to monitor environmental fluoride exposure and highlight the potential for therapeutic strategies targeting cholinergic, neurotrophic and glutamatergic pathways to mitigate fluoride-induced neuronal injury. - Source: PubMed
Dahiru AshiruNawaz IsmatRiaz Syeda KiranBangash NazneenAbbas MuhammadChaudry Summaya SohailKhan Muhammad JadoonHuang QingyuEqani Syed Ali Musstjab Akber Shah - Neurological disorders such as Alzheimer's disease and epilepsy are complex, multifactorial conditions that require therapeutic agents capable of acting through multiple mechanisms. In the present study, a series of structurally diverse flavonol analogues was synthesized and evaluated for their dual cholinesterase inhibitory and anticonvulsant potential through integrated , , and approaches. The compounds were screened for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities using standard spectrophotometric assays. Selected derivatives demonstrating significant enzyme inhibition were further assessed for anticonvulsant efficacy in maximal electroshock seizure (MES) and pentylenetetrazole (PTZ)-induced seizure models. Several compounds exhibited noteworthy inhibition of both cholinesterase enzymes, with the most active derivatives displaying low micromolar potency. In anticonvulsant studies, the leading compounds produced dose-dependent protection against electrically and chemically induced seizures, significantly reducing seizure severity, prolonging seizure latency, and increasing protection rates in both experimental models. To the best of our knowledge, this is among the first reports evaluating the anticonvulsant activity of this specific flavonol scaffold in both MES and PTZ models. Computational investigations, including induced-fit docking, MM-GBSA binding free-energy calculations, molecular dynamics simulations, ADME prediction, and density functional theory (DFT) analyses, revealed favorable enzyme-binding interactions, stable ligand-protein complexes, and acceptable drug-like characteristics. The convergence of biological and computational findings identified compound 8 as the most promising multifunctional candidate, exhibiting potent dual cholinesterase inhibition, pronounced anticonvulsant activity, and sustained target engagement. These results demonstrate that flavonol-based scaffolds represent attractive lead structures for the development of multifunctional therapeutic agents targeting neurodegenerative disorders and epilepsy. - Source: PubMed
Publication date: 2026/09/04
Mughal Ehsan UllahNaeem NafeesaKılınç NamıkAhmed IshtiaqZaki Magdi E AFathalla MaherSadiq AminaHefnawy Mohamed - Natural products rich in phenolic compounds are increasingly investigated as multifunctional agents against oxidative stress and enzyme-related disorders. This study characterized the phytochemical composition and evaluated the antioxidant and enzyme inhibitory activities of methanol and aqueous extracts of Stachys aleurites. Total phenolic and flavonoid contents were determined spectrophotometrically, while selected phenolics were quantified by LC-ESI-MS/MS. Antioxidant and enzyme inhibitory properties were assessed using complementary in vitro assays. The methanol extract contained higher levels of total phenolics (48.86 mg GAE/g) and flavonoids (65.36 mg RE/g) than the aqueous extract. Verbascoside and chlorogenic acid were the predominant compounds in the methanol extract, whereas p-coumaric acid was relatively more abundant in the aqueous extract. The methanol extract exhibited stronger antioxidant activity in most assays and showed greater inhibition of AChE, tyrosinase, α-amylase, and α-glucosidase. In contrast, the aqueous extract displayed slightly higher metal-chelating activity and stronger BChE inhibition. These findings demonstrate solvent-dependent variations in phytochemical composition and bioactivity, highlighting the importance of extraction solvent selection and supporting further studies on the bioactive constituents of S. aleurites. - Source: PubMed
Solmaz Fatma Ozlem KarginSarikurkcu CengizTepe Bektaş - Human carboxylesterase 1 (hCE1) is highly expressed in the liver and can be released into the bloodstream upon hepatocellular injury, suggesting its potential as a biomarker for liver damage. However, current methods for measuring hCE1 activity suffer from poor selectivity and insufficient sensitivity and are easily interfered with by serum albumin (HSA), butyrocholinesterase (BChE), and paraoxonase 1 (PON1). In this study, a novel fluorescent probe, NCMNe, was designed and synthesized, and an ultrasensitive liquid chromatography-fluorescence detection (LC-FD) method was established for quantifying plasma hCE1 activity and evaluating its feasibility as a biomarker of liver injury. Specificity studies showed that NCMNe was selectively hydrolyzed by hCE1 without interference from HSA, PON1, or BChE. The developed LC-FD method achieved a lower limit of quantification of 0.0001 μM, representing the most sensitive method reported to date for hCE1 activity determination. Clinical sample analysis revealed that plasma hCE1 activity in patients with hepatitis B was significantly higher than that in healthy controls and was strongly correlated with aspartate transaminase (AST)and alanine transaminase (ALT). Receiver operating characteristic (ROC) curve analysis demonstrated that plasma hCE1 activity measurement was effective for detecting liver pathology (AUC = 0.901, p < 0.0001), with diagnostic performance comparable to that of ALT and AST. Collectively, the proposed LC-FD method enables highly sensitive and selective determination of plasma hCE1 activity and supports hCE1 as a promising serum biomarker for liver injury, providing a new approach for early diagnosis and disease assessment. - Source: PubMed
Publication date: 2026/08/29
Zhao XuanWang Qian-QianZhang Qi-ChenXu XinWang Dan-Dan - This study presents the phytochemical and biological evaluation of the species Hedysarum yilmazunalii Kandemir, F. Yıldız & H.İ. Turkoğlu. Methanolic extracts from leaves, stems, and roots were characterized by LC-MS/MS, and their antioxidant, enzyme inhibitory, and antiproliferative activities were investigated. The analyses showed organ-specific differences in phenolic composition, with quinic acid as the major compound in leaves together with higher levels of flavonoid glycosides, including astragalin and isoquercitrin. Aerial extracts showed strong enzyme inhibitory activity. Leaf and stem extracts exhibited cholinesterase inhibition, with IC values of 5.82 and 6.42 µg/mL for AChE and 5.41 and 5.50 µg/mL for BChE, respectively. The leaf extract showed α-glucosidase inhibitory activity (4.10 µg/mL), comparable to acarbose, and antioxidant activity (DPPH: 6.46 µg/mL), together with the highest phenolic (145.43 mg GAE/g extract) and flavonoid contents (87.20 mg QE/g extract). Antiproliferative assays demonstrated growth inhibition of lung and breast cancer cell lines, with the lowest GI values of 1.00 µg/mL for H1650 and 2.34 µg/mL for MDA-MB-231 cells. LDH cytotoxicity remained low (2.25%-12.59%), indicating limited membrane damage in both cancer and normal cells. These findings identify H. yilmazunalii as a source of compounds with antioxidant, neuroprotective, antidiabetic, and anticancer potential. - Source: PubMed
Osma EtemVarol TuğçeŞimşek SamedAltın SevgiYılmaz Mustafa AbdullahAydın AliElveren MüjganKandemir Ali