Ask about this productRelated genes to: BAG5 Blocking Peptide
- Gene:
- BAG5 NIH gene
- Name:
- BCL2 associated athanogene 5
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 14q32.33
- Locus Type:
- gene with protein product
- Date approved:
- 1999-04-23
- Date modifiied:
- 2016-10-05
Related products to: BAG5 Blocking Peptide
Related articles to: BAG5 Blocking Peptide
- BackgroundPhysical exercise shows neuroprotective and anti-inflammatory effects in Alzheimer's disease (AD) models, but whether it modulates neuroinflammation through regulation of peripheral T-cell activity is still unresolved.ObjectiveThe present study aimed to explore the mechanisms by which aerobic exercise regulates peripheral T cell-mediated immune responses and their potential contribution to neuroinflammation in AD.MethodsMale wild-type mice and APP/PS1 transgenic mice were divided into four groups: wild-type sedentary mice (WT-SE) group, wild-type exercise group (WT-EX), APP/PS1 transgenic AD sedentary mice (AD-SE) group, and APP/PS1 transgenic AD exercise mice (AD-EX) group. The sedentary groups received no exercise training, while the exercise groups underwent a 3-month treadmill aerobic exercise intervention. At the end of the intervention, T lymphocytes were isolated from spleens. Label-free proteomics combined with LC-MS/MS was used to identify differentially expressed proteins (DEPs) and perform functional and pathway enrichment analyses. Differentially expressed protein-coding genes were validated at the mRNA level using RT-qPCR.ResultsA total of 3399 proteins were quantified across the four groups. Applying a threshold of |log fold change| > 0.67 and < 0.05, 913 DEPs were identified. These DEPs were significantly enriched in biological processes including immune system processes, protein-containing complexes, and structural molecule activity, as well as signaling pathways including AD, TGF-β, and apoptosis.ConclusionsOverall, HSP90AB1, HSP90AA1, BAG5, DNAJC8, CTSD, and ANXA1 may play a role in peripheral T-cell immune dysregulation in AD, with potential implications for central neuroinflammation. Furthermore, the beneficial effects of aerobic exercise on AD-related peripheral immune alterations, and its potential modulation of neuroinflammation, may be associated with expression changes in DEPs including Ppp2r1b, Pde2a, Casp8, Apaf-1, Dnajb11, and Dnajc13. - Source: PubMed
Publication date: 2026/03/30
Ye XingHu KaiLiu Renyi - The epididymis orchestrates sperm maturation through microenvironmental regulation and epididymosome-mediated cargo delivery. Despite emerging evidence implicating protein S-acylation in vesicular trafficking, its compartment-specific dynamics and functional implications in epididymal physiology remain poorly characterized. Here, we employed acyl-biotin exchange-based 4D proteomics to decode the S-acylation proteomic of porcine caput/cauda epididymidis and their exosomes. Comparative analysis identified 2780 and 2084 S-acylated proteins in caput and cauda tissues, respectively, with 317 upregulated and 579 downregulated S-acylated proteins in cauda versus caput. Functional enrichment revealed S-acylation-dependent regulation of signal transduction, vesicle trafficking, and immune pathways, particularly through lysosomal activity, AMPK signaling, and glutathione metabolism. Exosomal profiling demonstrated conserved S-acylated protein signatures between caput and cauda derived exosomes, with 114 S-acylated proteins shared among caput tissue and both exosomal populations, implicating long-distance transport of caput-specific cargoes. Validation identified 5 caput-enriched S-acylated proteins, including evolutionarily conserved OCLN, CDH1, PDZK1, BAG5, and SCRN1, which were detected in S-acylated forms within caput-derived exosomes and cauda exosomes, but absent in cauda tissue. This study reveals a potential role of S-acylation in mediating exosomal cargo trafficking during porcine epididymis. Our findings advance understanding of post-testicular sperm functionalization and highlight S-acylation as a potential therapeutic target for male infertility. SIGNIFICANCE: This study provides the comprehensive S-acylation proteomic atlas of the porcine epididymis and its exosomes, revealing how this reversible lipid modification spatiotemporally regulates exosome-mediated protein trafficking to support sperm maturation. We demonstrate that S-acylation governs key pathways including vesicle transport, immune regulation, and metabolic signaling in the epididymal microenvironment. Crucially, we identify a cohort of caput-enriched S-acylated proteins that are packaged into exosomes and transported distally to the cauda region, suggesting a previously unrecognized mechanism for long-distance intercellular communication. These findings establish S-acylation as a central regulator of epididymal function and offer molecular insights into post-testicular sperm maturation. The identified S-acylated proteins and associated pathways may serve as diagnostic biomarkers or therapeutic targets for male infertility, particularly in cases of defective sperm functionalization. - Source: PubMed
Publication date: 2026/03/19
Cao HeranLiu XiaohuaLi LongLiu ShujuanGao HuihuiDong QianNie HuaLi YanGong YeJin TianqiWang YangQin WeibingDong Wuzi - Male infertility represents a major global health challenge. Heat shock protein A1A (HSPA1A), a stress-inducible molecular chaperone, shows potential importance in spermatogenesis, though its precise mechanistic role remains undefined. - Source: PubMed
Publication date: 2026/01/17
Yi PengPeng BoCao YingFu Xianghong - Bactrian camels show strict seasonal estrus, yet its regulatory mechanisms remain largely unknown. This process involves multi-gene regulation, where ncRNAs (miRNAs, lncRNAs/circRNAs) critically regulate cellular functions. These ncRNAs interact to form competitive endogenous RNA (ceRNA) networks that influence the the cell cycle, proliferation, and apoptosis. In this study, transcriptional sequencing of Bactrian camel testicles identified and validated key seasonal estrus-associated DEGs, lncRNAs, and miRNAs via seq-RNA. Among the identified genes, BAG5, which is associated with testicular development and apoptosis, exhibited significantly higher mRNA and protein levels during anestrus, as determined by qRT-PCR and Western blot. Dual luciferase assays confirmed BAG5 and lncRNA MSTRG.118323.5 as miR-200-y targets. In vitro experiments with miR-200-y mimics and inhibitors confirmed their negative regulation of both BAG5 and lncRNA expression, validated by qPCR (transcripts) and Western blot/immunofluorescence (proteins). Further analysis revealed that BAG5 modulates the PI3K/AKT/mTOR pathway, influencing apoptosis-related proteins. Flow cytometry results indicated that MSTRG.118323.5 binds competitively to miR-200-y, thereby enhancing BAG5' s anti-apoptotic effects. This study reveals a regulatory network involving lncRNA-miRNA-BAG5 in testicular apoptosis and provides novel insights into the molecular mechanisms underlying seasonal estrus and testicular development in Bactrian camels. These findings contribute to a deeper understanding of reproductive regulation in desert-adapted species. - Source: PubMed
Publication date: 2025/09/11
Nan JinghongLiu YuanyuanWang RuiqingYan QiuZhang YongWang QiZhao Xingxu - With the continuous emergence of new technologies in omics, the integrative analysis of multi-omics data has become a new direction to explore life mechanisms. The Bcl-2 associated athanogene (BAG) family consists of co-chaperones involved in various cellular processes, including stress signaling, cell cycle regulation, and tumorigenesis. BAG5, a unique member of this family, contains multiple BAG domains, yet its role in non-small cell lung cancer (NSCLC) remains largely unexplored. - Source: PubMed
Publication date: 2025/07/25
Huang Jing-ShanWang Jia-MeiYuan YeZhang TingLi Bai-QiangZhao Fu-YingHao LiangYu Zhan-WuWang Hua-Qin