Ask about this productRelated genes to: BAG5 Blocking Peptide
- Gene:
- BAG5 NIH gene
- Name:
- BCL2 associated athanogene 5
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 14q32.33
- Locus Type:
- gene with protein product
- Date approved:
- 1999-04-23
- Date modifiied:
- 2016-10-05
Related products to: BAG5 Blocking Peptide
Related articles to: BAG5 Blocking Peptide
- Avian influenza A virus (IAV) is a major global health threat. Not only does it cause significant economic losses to the agricultural industry, but it also presents a risk of spillover into the human population. However, several obstacles must be overcome for a successful cross-species transmission event to occur. One of these obstacles is the innate immunity of the host. While the innate immune response to IAV in humans has been well-studied, the innate immune response to IAV in birds is not as well understood. Here, we explore the interferon-induced immune response by identifying interferon-stimulated genes (ISGs) in chicken, quail, and duck cells, and find that they are largely species-specific. Using this data, we performed a CRISPR knockout ISG screen against two strains of IAV, the mouse-adapted PR8, and the low pathogenicity avian IAV isolate WF10. We find that for PR8, IFITM3 is the dominant restrictor of viral replication with several additional hits with modest effects, consistent with previous work in mammals. For avian WF10, IFITM3 was also a hit however there were two additional hits absent for PR8, the HSP70 co-chaperone protein BAG5 and deubiquitinase OTUD4. Overall, these findings confirm the divergence of interferon-mediated immunity between species and establish a screening system that can be used to identify ISGs important for viral restriction in avian species. - Source: PubMed
Publication date: 2026/07/24
Barkhymer Alison JBecker Jordan TShepherd Frances KMickelson Clayton KPross Lauren MSalnikov MarkLanglois Ryan A - Colorectal cancer (CRC) ranks among the most prevalent malignancies globally, and radiotherapy remains a critical treatment modality. However, its efficacy is frequently compromised by acquired radioresistance. The endoplasmic reticulum chaperone protein BIP plays a pivotal role in regulating radioresistance by coordinating the balance between protective autophagy and apoptosis, though the regulatory roles of circular RNAs (circRNAs) in this process remain poorly understood. - Source: PubMed
Publication date: 2026/06/21
Chen WangshengZhang XuZhao ZhengfeiXin WangLi JianxinChe Xiangming - The integrated stress response (ISR) can cause high-grade serous ovarian cancer (HGSOC) cells to arrest in G1 phase, significantly suppressing their hyperproliferation. Nevertheless, the specific molecular mechanisms of the ISR in HGSOC remain unclear. - Source: PubMed
Publication date: 2026/05/05
Li QianKong FanqiangCui PenghuaGao XiujuanZhuang XinrongZhang ZhongkaiTian TianZhang Guixiang - BackgroundPhysical exercise shows neuroprotective and anti-inflammatory effects in Alzheimer's disease (AD) models, but whether it modulates neuroinflammation through regulation of peripheral T-cell activity is still unresolved.ObjectiveThe present study aimed to explore the mechanisms by which aerobic exercise regulates peripheral T cell-mediated immune responses and their potential contribution to neuroinflammation in AD.MethodsMale wild-type mice and APP/PS1 transgenic mice were divided into four groups: wild-type sedentary mice (WT-SE) group, wild-type exercise group (WT-EX), APP/PS1 transgenic AD sedentary mice (AD-SE) group, and APP/PS1 transgenic AD exercise mice (AD-EX) group. The sedentary groups received no exercise training, while the exercise groups underwent a 3-month treadmill aerobic exercise intervention. At the end of the intervention, T lymphocytes were isolated from spleens. Label-free proteomics combined with LC-MS/MS was used to identify differentially expressed proteins (DEPs) and perform functional and pathway enrichment analyses. Differentially expressed protein-coding genes were validated at the mRNA level using RT-qPCR.ResultsA total of 3399 proteins were quantified across the four groups. Applying a threshold of |log fold change| > 0.67 and < 0.05, 913 DEPs were identified. These DEPs were significantly enriched in biological processes including immune system processes, protein-containing complexes, and structural molecule activity, as well as signaling pathways including AD, TGF-β, and apoptosis.ConclusionsOverall, HSP90AB1, HSP90AA1, BAG5, DNAJC8, CTSD, and ANXA1 may play a role in peripheral T-cell immune dysregulation in AD, with potential implications for central neuroinflammation. Furthermore, the beneficial effects of aerobic exercise on AD-related peripheral immune alterations, and its potential modulation of neuroinflammation, may be associated with expression changes in DEPs including Ppp2r1b, Pde2a, Casp8, Apaf-1, Dnajb11, and Dnajc13. - Source: PubMed
Publication date: 2026/03/30
Ye XingHu KaiLiu Renyi - The epididymis orchestrates sperm maturation through microenvironmental regulation and epididymosome-mediated cargo delivery. Despite emerging evidence implicating protein S-acylation in vesicular trafficking, its compartment-specific dynamics and functional implications in epididymal physiology remain poorly characterized. Here, we employed acyl-biotin exchange-based 4D proteomics to decode the S-acylation proteomic of porcine caput/cauda epididymidis and their exosomes. Comparative analysis identified 2780 and 2084 S-acylated proteins in caput and cauda tissues, respectively, with 317 upregulated and 579 downregulated S-acylated proteins in cauda versus caput. Functional enrichment revealed S-acylation-dependent regulation of signal transduction, vesicle trafficking, and immune pathways, particularly through lysosomal activity, AMPK signaling, and glutathione metabolism. Exosomal profiling demonstrated conserved S-acylated protein signatures between caput and cauda derived exosomes, with 114 S-acylated proteins shared among caput tissue and both exosomal populations, implicating long-distance transport of caput-specific cargoes. Validation identified 5 caput-enriched S-acylated proteins, including evolutionarily conserved OCLN, CDH1, PDZK1, BAG5, and SCRN1, which were detected in S-acylated forms within caput-derived exosomes and cauda exosomes, but absent in cauda tissue. This study reveals a potential role of S-acylation in mediating exosomal cargo trafficking during porcine epididymis. Our findings advance understanding of post-testicular sperm functionalization and highlight S-acylation as a potential therapeutic target for male infertility. SIGNIFICANCE: This study provides the comprehensive S-acylation proteomic atlas of the porcine epididymis and its exosomes, revealing how this reversible lipid modification spatiotemporally regulates exosome-mediated protein trafficking to support sperm maturation. We demonstrate that S-acylation governs key pathways including vesicle transport, immune regulation, and metabolic signaling in the epididymal microenvironment. Crucially, we identify a cohort of caput-enriched S-acylated proteins that are packaged into exosomes and transported distally to the cauda region, suggesting a previously unrecognized mechanism for long-distance intercellular communication. These findings establish S-acylation as a central regulator of epididymal function and offer molecular insights into post-testicular sperm maturation. The identified S-acylated proteins and associated pathways may serve as diagnostic biomarkers or therapeutic targets for male infertility, particularly in cases of defective sperm functionalization. - Source: PubMed
Publication date: 2026/03/19
Cao HeranLiu XiaohuaLi LongLiu ShujuanGao HuihuiDong QianNie HuaLi YanGong YeJin TianqiWang YangQin WeibingDong Wuzi