PLAC1L Blocking Peptide
- Known as:
- PLAC1L Blocking Peptide
- Catalog number:
- 33r-10184
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- PLAC1L Blocking Peptide
Related genes to: PLAC1L Blocking Peptide
- Gene:
- OOSP2 NIH gene
- Name:
- oocyte secreted protein 2
- Previous symbol:
- TMEM122, PLAC1L
- Synonyms:
- FLJ36198, OOSP2A
- Chromosome:
- 11q12.1
- Locus Type:
- gene with protein product
- Date approved:
- 2006-02-02
- Date modifiied:
- 2018-06-07
Related products to: PLAC1L Blocking Peptide
Related articles to: PLAC1L Blocking Peptide
- Ovarian aging is characterized by a progressive decline in oocyte quality and quantity with age. Icariin (ICA), a flavonoid compound derived from species, has demonstrated potential as an agent for ovarian restoration. In this study, a subcutaneous implantation system using gelatin methacryloyl (GelMA) hydrogel embedded with ICA was developed to restore ovarian function in aged female mice. Mice were assigned to receive subcutaneous implantation of GelMA alone (GelMA group), GelMA containing ICA (GelMA/ICA group), or a sham operation. Ovarian morphology, serum hormone levels, follicle counts across developmental stages, and reproductive outcomes were evaluated. fertilization (IVF) and embryo culture assays were performed to assess oocyte developmental potential, while a 10 day natural mating trial was conducted to determine fertility restoration. RNA sequencing (RNA-seq) and RT-qPCR were performed to elucidate the underlying molecular mechanisms. Results showed that GelMA/ICA treatment significantly increased ovarian index (0.19±0.01 vs. 0.13±0.01, <0.0001) and follicle numbers at all developmental stages, including primordial (383.33±151.65 vs. 107.14±32.26, <0.0001), primary (203.33±83.22 vs. 91.43±27.04, =0.003), and secondary follicles (154.17±52.00 vs. 59.28±20.50, =0.029) compared to the sham controls. Hormonal analyses revealed a significant reduction in serum follicle-stimulating hormone (FSH, 11.97±3.53 vs. 53.10±17.89 ng/mL, =0.0008), accompanied by elevated anti-Müllerian hormone (AMH, 22.97±2.26 vs. 5.54±1.56 ng/mL, <0.0001) and estradiol (E , 315.30±37.62 vs. 168.5±14.78 pg/mL, <0.0001). Oocyte yield and developmental potential improved significantly, as reflected by the increased number of superovulated MII oocytes (17.83±5.15 vs. 4.83±4.79, =0.0002), and higher proportions of two-cell (85.90%±6.16% vs. 50.00%±10.00%, =0.0009), four-cell (81.67%±9.76% vs. 50.00%±10.00%, =0.0061), and blastocyst stage embryos (64.25%±10.55% vs. 23.33%±15.28%, =0.0067). Live birth numbers were significantly increased following GelMA/ICA treatment (6.90±3.21 vs. 1.72±2.05, =0.0001). Transcriptomic analysis revealed up-regulation of genes associated with cytoskeletal organization ( , ), lipid storage ( , ), oocyte maturation ( ), and cytokine secretion ( ). Collectively, these findings suggest that GelMA/ICA hydrogels effectively reverse key hallmarks of ovarian aging and restore reproductive function in aged mice, offering a promising platform for fertility preservation and a novel therapeutic for future investigations into ovarian aging. - Source: PubMed
Mao Jia-LianWu Xiang-YiLi Ling-XiLi NingWang Ya-XuanJiang Zhi-WeiLiu Chuan-MingZhang Hui-DanZhou Ji-DongZhang YangChen LiYan Gui-JunSun Hai-XiangLi Yi-FanDing Li-Jun - Bovine spastic paresis (BSP) is a progressive neuromuscular disease of unknown origin that causes persistent stiffness of the hind limbs. The symptoms are similar to those of human motor neuron diseases such as primary (PLS) or amyotrophic lateral sclerosis (ALS). BSP occurs worldwide in cattle production with an estimated prevalence of <1%. For Germany, this means that around 20,000 Holstein cattle are affected. BSP is generally considered a hereditary disease, but there is no prevention through breeding programs. As a result, BSP not only affects animal welfare but also leads to economic losses in milk and beef production. Here, we used transcriptomics to analyse the brainstem, spinal cord and affected gastrocnemius muscle tissue of eight animals affected by BSP and eight control animals from slaughterhouses to gain new insights into the molecular mechanisms underlying BSP. We found that the expression of several genes was significantly different in animals affected by BSP compared to control animals. Specific genes for inhibitory neurons were downregulated in the brainstems of the affected animals, namely CCK (cholecystokinin), NPY (neuropeptide Y), and SST (somatostatin). These inhibitory neurotransmitters influence cerebral movement control, among other processes. Furthermore, OOSP2 (oocyte secreted protein 2) was found to be significantly upregulated in the affected animals in all tissues. This expression could best be explained by the presence of T-follicular-helper cells which, through interleukin 21, can trigger a TH-2-dominated immune response and lead to autoimmune encephalitis. Further cases were sampled for confirmation and we detected cell infiltrates of activated microglia and T-cells in the brainstem using immunohistochemistry. Microglial foci were significantly more abundant in animals affected by BSP than control animals. We conclude that BSP is caused by an autoimmune reaction directed against inhibitory interneurons in the brainstem and is due to a combination of genetics and environmental influences. This may result in lost controlling influence on the upper motor neurons via extrapyramidal pathways and therefore triggers the specific symptoms of motor neuron disease. - Source: PubMed
Publication date: 2025/05/29
Krull FrederikHosseini ShahrbanouBleyer MartinaBrenig Bertram - The combined use of transcriptome and translatome as indicators of gene expression profiles is usually more accurate than the use of transcriptomes alone, especially in cell types governed by translational regulation, such as mammalian oocytes. Here, we developed a dual-omics methodology that includes both transcriptome and translatome sequencing (T&T-seq) of single-cell oocyte samples, and we used it to characterize the transcriptomes and translatomes during mouse and human oocyte maturation. T&T-seq analysis revealed distinct translational expression patterns between mouse and human oocytes and delineated a sequential gene expression regulation from the cytoplasm to the nucleus during human oocyte maturation. By these means, we also identified a functional role of OOSP2 inducing factor in human oocyte maturation, as human recombinant OOSP2 induced in vitro maturation of human oocytes, which was blocked by anti-OOSP2. Single-oocyte T&T-seq analyses further elucidated that OOSP2 induces specific signaling pathways, including small GTPases, through translational regulation. - Source: PubMed
Publication date: 2022/08/30
Hu WenqiZeng HaitaoShi YananZhou ChuanchuanHuang JianaJia LeiXu SiqiFeng XiaoyuZeng YanyanXiong TuanlinHuang WenzeSun PengChang YajieLi TingtingFang CongWu KeliangCai LingboNi WuhuaLi YanYang ZhiyongZhang Qiangfeng CliffChian RiChengChen ZijiangLiang XiaoyanKee Kehkooi - The occupant's posture can be changeable to an inadvertent or unintentional out-of-seat position (OOSP) depend on their convenience. Understanding for OOSP has been demanded, but it is not sufficient; especially when AEB is activated. The aim of the current study was to characterize the motion responses of an occupant in various OOSPs when AEB is activated and to identify if there were any additional risks of injury or discomfort to the occupant. The normal seat position (NSP) and three OOSPs were defined to compare the difference of human responses, and six healthy males were participated. Particularly, the maximum rotation angles of the neck in OOSP2 and OOSP3 differed significantly around 1.3 ± 0.3 and 1.4 ± 0.2 times higher respectively than from in the NSP (p < 0.05). Occupants assuming OOSP3 exhibited motion characteristics were not restrained effectively and characterized a hovering and falling upper body and a slipping pelvis. This study has identified, for the first time, a potential risk of injury or discomfort when AEB is activated while an occupant is in an OOSP. This study may serve as fundamental data for the development of safety system that can improve restraint and counteract any deterioration in occupant safety. - Source: PubMed
Publication date: 2021/06/10
Kang MyeongkwanLee InjuJung JisooKim SeonglaeCho YoungkuenKim HyungjooLim Dohyung - There are over 200 genes that are predicted to be solely expressed in the oocyte and ovary, and thousands more that have expression patterns in the female reproductive tract. Unfortunately, many of their physiological functions, such as their roles in oogenesis or fertilization, have yet to be elucidated. Previous knockout (KO) mice studies have proven that many of the genes that were once thought to be essential for fertility are dispensable in vivo. Therefore, it is extremely important to confirm the roles of all genes before spending immense time studying them in vitro. To do this, our laboratory analyzes the functions of ovary and oocyte-enriched genes in vivo through generating CRISPR/Cas9 KO mice and examining their fertility. In this study, we have knocked out three family genes (, , and ) that have expression patterns linked to the female reproductive system and found that the triple KO (TKO) mutant mice generated exhibited decreased prolificacy but were not infertile; thus, these genes may potentially be dispensable for fertility. We also generated and KO mice and found these genes are individually dispensable for female fertility. KO mice with no phenotypic data are seldom published, but we believe that this information must be shared to prevent unnecessary experimentation by other laboratories. - Source: PubMed
Publication date: 2020/03/28
Abbasi FerheenKodani MayoEmori ChihiroKiyozumi DaijiMori MasashiFujihara YoshitakaIkawa Masahito