Ask about this productRelated genes to: RSAD2 Blocking Peptide
- Gene:
- RSAD2 NIH gene
- Name:
- radical S-adenosyl methionine domain containing 2
- Previous symbol:
- -
- Synonyms:
- cig5, viperin, vig1
- Chromosome:
- 2p25.2
- Locus Type:
- gene with protein product
- Date approved:
- 2004-11-08
- Date modifiied:
- 2014-11-19
Related products to: RSAD2 Blocking Peptide
Related articles to: RSAD2 Blocking Peptide
- Prostate adenocarcinoma is characterized by substantial inter-patient heterogeneity, limiting the clinical reliability of conventional diagnostic tools, including prostate-specific antigen testing. This limitation underscores the need for robust molecular biomarkers that may complement conventional diagnostic tools, highlighting the urgent need for biomarkers capable of enhancing diagnostic accuracy and enabling more precise risk stratification. In the present study, transcriptomic data from The Cancer Genome Atlas (TCGA) were analyzed using an integrative bioinformatics and machine learning pipeline., The proposed workflow was designed as a stepwise and reproducible biomarker prioritization framework in which differential expression analysis, functional enrichment, protein-protein interaction (PPI) based network interpretation, graph-convolutional feature selection, and hybrid ensemble machine learning were sequentially integrated. Differential gene expression analysis was combined with pathway enrichment (Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome), protein-protein interaction network construction, and graph-convolutional feature selection. Multiple machine learning algorithms, including Random Forest, Gradient Boosting Machine, Support Vector Classifier, Artificial Neural Network, and AdaBoost, were systematically evaluated. A hybrid ensemble model integrating Gradient Boosting Machine and Random Forest (GBM+RF) was subsequently developed. Model performance was assessed using accuracy, sensitivity, specificity, and area under the Receiver Operating Characteristic (ROC) and externally validated using the independent GSE14206 dataset. The analysis revealed a coordinated molecular pattern characterized by dysregulated cell cycle activity and enhanced interferon-mediated immune signaling. Protein-protein interaction analysis identified and as highly connected network hub genes within immune-related and cell-cycle-associated modules. Among the evaluated models, the hybrid GBM+RF framework achieved the highest predictive performance on the TCGA dataset, with AUC: 0.9526; Accuracy: 97.49%. External validation using the GSE14206 dataset confirmed the robustness of this model (AUC: 0.9156; Accuracy: 91.53%). These findings support a broader multi-gene candidate signature in prostate adenocarcinoma, in which machine learning prioritized genes such as , , , , , , , and , while and provided complementary network-level biological relevance. The proposed framework provides a robust and transferable strategy for biomarker discovery and precision oncology. - Source: PubMed
Publication date: 2026/07/25
Kurt Hasan AnılKılıçarslan SabireÇiçekliyurt Meliha MerveKılıçarslan Serhat - Type IV interferon (IFN), a recently identified class of IFN, exhibits broad-spectrum antiviral and antibacterial functions across species. In this study, the type IV IFN gene, IFN-υ, and its cognate receptor subunits (IFN-υR1 and IL10RB) were functionally characterized in an avian species, the rock pigeon (Columba livia). Phylogenetic analysis showed that rock pigeon IFN-υ, IFN-υR1, and IL10RB were clustered closely with their respective orthologs in other vertebrates. Expression profiling revealed the constitutive transcription of these genes across multiple tissues. Furthermore, the expression of these genes was significantly upregulated upon stimulation with the viral double-stranded RNA (dsRNA) mimic, poly(I:C). The recombinant rock pigeon IFN-υ activated the canonical Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway, inducing STAT1 phosphorylation and the consequent expression of key interferon-stimulated genes (ISGs) such as OAS1, PKR, and RSAD2. Using overexpression and knockdown assays, the biological activity of IFN-υ was found to signal through a heterodimeric receptor complex comprising IFN-υR1 and IL10RB. Importantly, two conserved tyrosine residues (Tyr and Tyr) within the intracellular domain of IFN-υR1 were found essential for IFN-υ-mediated JAK-STAT pathway activation, ISG induction, and antiviral activity. These findings elucidate the conserved functional architecture and signaling mechanism of type IV IFN in vertebrates. - Source: PubMed
Publication date: 2026/08/12
Pang An NingLi Jia YiChen Shan NanWu Xiang YangLi NanLiu Lan HaoNie P - Epstein-Barr Virus (EBV) infection and reactivation in B-lymphocytes are tightly regulated by host antiviral response genes. In the present study, we identify Interferon Stimulated Genes (ISGs) RSAD2 (radical S-adenosyl methionine domain-containing 2) and CMPK2 (Cytidine/Uridine Monophosphate Kinase 2) as key modulators of EBV expression and cellular response during EBV infection and reactivation. EBV primary infection and reactivation lead to a coordinated upregulation of RSAD2 and CMPK2. Depletion of RSAD2 reduced cell viability and limited EBV reactivation, while depletion of CMPK2 led to reactivation of EBV lytic gene expression during latency. Despite distinct subcellular localizations, RSAD2 at the endoplasmic reticulum (ER) and CMPK2 in the mitochondria, transcriptomic analysis revealed that both genes functionally converge and exhibit overlapping roles in driving shared immunometabolic pathways, specifically Interferon (IFN) signaling, MAPK signaling, oxidative phosphorylation, mitochondrial function, eukaryotic translation, and ATF-4-associated unfolded protein response (UPR). We show that RSAD2 and CMPK2 knockdown affects IRAK1-TRAF6-TAK1 expression levels, and RSAD2 overexpression downregulates NF-κB signaling by EBV membrane associated oncoprotein LMP1. Depletion of RSAD2 and CMPK2 had significant effects on global metabolites consistent with a remodeling of nucleotide metabolism, glycolysis, fatty acid biosynthesis and degradation of superoxides. EBV reactivation induced formation of antiviral ribonucleotide ddhCTP which was strictly dependent on RSAD2. These observations demonstrate that RSAD2 and CMPK2 function in a coordinated ER-Mitochondria-Interferon signaling axis that shapes EBV reactivation and host immune control, including a novel layer of immunometabolic regulation modulating viral latency and reactivation. - Source: PubMed
Publication date: 2026/08/03
Zankharia Urvi SGlass Adam MZhang WujuanZhu QingMachhar Janvhi SureshYe YingKarisetty Bhanu ChandraWickramasinghe JayamannaKossenkov AndrewMadzo JozefChung Sun SookSoldan Samantha SLamontagne Richard JasonWood James MHarris Lawrence DGrove Tyler LJacobson StevenGoldman Aaron RLiang ChengyuLieberman Paul M - Embryo implantation is a complex biological process whose success depends on synchrony between a competent embryo and a receptive endometrium. In this study, we investigated the regulatory role of Colony-Stimulating Factor 1 (CSF1) in modulating endometrial function during early pregnancy in goats. CSF1 expression was significantly upregulated during early gestation. Functionally, CSF1 overexpression in goat endometrial epithelial cells (gEECs) markedly enhanced the expression of epithelial-mesenchymal transition (EMT) markers, including Vimentin and N-cadherin. In hormone-treated gEECs, CSF1 regulated RSAD2 expression but did not significantly affect ISG15 or CXCL10. Conversely, CSF1 silencing attenuated these effects. Transcriptomic analysis implicated the P38-MAPK signaling pathway as a downstream mediator of CSF1-induced endometrial receptivity. Mechanistically, CSF1 increased P38 phosphorylation and partial EMT marker expression. Pharmacological activation of P38 using HY-B1204 rescued the downregulation of N-cadherin induced by CSF1 silencing, although Vimentin expression was not significantly restored. Collectively, these findings demonstrate that CSF1 is associated with activation of the P38-MAPK pathway, thereby regulating molecular characteristics of endometrial receptivity during early implantation in goats and providing new insights into CSF1-mediated mechanisms in ruminant reproduction. - Source: PubMed
Publication date: 2026/07/24
Yang SongjianTang QianHaoLiao YuHaoFang XingQiangZhao LeZhao Yongju - To evaluate the relationship between IFN-related gene (IRG) expression and disease activity, organ involvement and serological subgroups in JDM and to assess its potential as a biomarker for disease monitoring and treatment response. - Source: PubMed
Codes-Méndez HelenaCuyx SenneSyntakas Aris EBarmpakou AfroditiMoraitis ElenaCompeyrot-Lacassagne SandrineAl-Obaidi MuthanaPapadopoulou Charalampia