Ask about this productRelated genes to: SLC27A4 Blocking Peptide
- Gene:
- SLC27A4 NIH gene
- Name:
- solute carrier family 27 member 4
- Previous symbol:
- -
- Synonyms:
- FATP4, ACSVL4
- Chromosome:
- 9q34.11
- Locus Type:
- gene with protein product
- Date approved:
- 1999-08-20
- Date modifiied:
- 2016-10-05
Related products to: SLC27A4 Blocking Peptide
Related articles to: SLC27A4 Blocking Peptide
- Thyroid hormones are central regulators of metabolic homeostasis and developmental programming. The active hormone triiodothyronine (T3) modulates transcription through nuclear receptors that recruit epigenetic cofactors to remodel chromatin and regulate metabolic gene networks. Although thyroid hormone signaling is known to influence lipid metabolism, whether it coordinates lipid droplet turnover with autophagy-related pathways during early embryonic development remains largely unknown. Here, transcriptomic profiling revealed distinct metabolic signatures between and embryos, with marked differences in fatty acid metabolism. Supplementation with 50 nM T3 enhanced blastocyst formation, particularly when applied from the 4-cell to blastocyst stages, coinciding with elevated thyroid hormone receptor expression. T3 induced robust lipid droplet remodeling, characterized by reduced droplet size, together with increased lipid-mitochondria colocalization and activation of lysosomal and mitochondrial pathways, consistent with enhanced lipid catabolism and organelle coupling. Mechanistically, inhibition of the histone acetyltransferase KAT2B/PCAF abolished T3-mediated developmental gains, reduced H3K9ac and H3K27ac, and resulted in nonselective autophagic stress rather than lipophagy. By contrast, T3 required KAT2B to stimulate cytosolic lipolysis, channel fatty acids into mitochondria, and enhance mitochondrial membrane potential. T3 also upregulated prostaglandin biosynthesis genes and improved outgrowth performance. These findings identify a thyroid hormone-KAT2B epigenetic axis that coordinates lipid droplet remodeling through lipolytic and lipophagic pathways, linking endocrine signaling to organelle crosstalk and mitochondrial activation during early embryogenesis. ART: assisted reproductive technology; ATG5: autophagy related 5; BSA: bovine serum albumin; BSCL2/SEIPIN: BSCL2 lipid droplet biogenesis associated, seipin; CARM1: coactivator associated arginine methyltransferase 1; COCs: cumulus-oocyte complexes; CPT1A: carnitine palmitoyltransferase 1A; CPT2: carnitine palmitoyltransferase 2; CREBBP/CBP: CREB binding protein; DEGs: differentially expressed genes; DGAT1: diacylglycerol O-acyltransferase 1; EP300: E1A binding protein p300; ER: endoplasmic reticulum; H3K9ac: histone H3 acetyl-Lys9; H3K27ac: histone H3 acetyl-Lys27; HCS: high-content screening; HDACs: histone deacetylases; IVC: in vitro culture; IVF: in vitro-fertilized embryos; IVM: in vitro maturation; IVO: in vivo embryos; KAT2A/GCN5: K(lysine) acetyltransferase 2A; KAT2B/PCAF: K(lysine) acetyltransferase 2B; KAT2Bi (Ki): KAT2B inhibition; KD: knockdown; LC3: microtubule associated protein 1 light chain 3; LDs: lipid droplets; LIPE/HSL: lipase E, hormone sensitive type; MGLL: monoglyceride lipase; MMP: mitochondrial membrane potential; mtDNA: mitochondrial DNA; MT-ND1: mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1; PA: parthenogenetically activated; PG: prostaglandin; PLA2G4A: phospholipase A2 group IVA; PLIN2: perilipin 2; PLIN3: perilipin 3; PLIN5: perilipin 5; PNPLA2/ATGL: patatin like phospholipase domain containing 2; PPARD/PPARδ: peroxisome proliferator activated receptor delta; PPARs: peroxisome proliferator activated receptors; PRMT1: protein arginine methyltransferase 1; PTGS1: prostaglandin-endoperoxide synthase 1; PTGS2: prostaglandin-endoperoxide synthase 2; PVA: polyvinyl alcohol; RT: room temperature; RT-qPCR: reverse transcription-quantitative polymerase chain reaction; RXR: retinoid X receptor; SIRT1: sirtuin 1; SLC25A20/CACT: solute carrier family 25 member 20; SLC27A4/FATP4: solute carrier family 27 member 4; SUV39H1: SUV39H1 histone lysine methyltransferase; T3: triiodothyronine; T4: thyroxine; TAGs: triacylglycerols; TEM: transmission electron microscopy; THR: thyroid hormone receptor; THRA: thyroid hormone receptor alpha; THRA-i: thyroid hormone receptor antagonist; THRB: thyroid hormone receptor beta; THs: thyroid hormones; ZGA: zygotic genome activation. - Source: PubMed
Publication date: 2026/09/24
Lee Song-HeeZhan Cheng-LinCui Xiang-Shun - Renal cell carcinoma (RCC) is the most common malignancy of the urinary system, characterized by high incidence, mortality, and resistance to therapy. Its molecular heterogeneity presents challenges for effective precision treatment. RCC is highly heterogeneous, yet treatment guidelines rely predominantly on kidney renal clear cell carcinoma (KIRC) studies, neglecting other molecular subtypes, which limits therapy personalization for non-KIRC patients. This study aimed to explore the role of small ubiquitin-like modifier (SUMOylation)-associated genes in the progression and prognosis of RCC and its subtypes. We identified 298 SUMOylation-associated differentially expressed genes (DEGs), including 151 RCC-specific genes after excluding expression changes attributable to RCC subtype-specific variation. Ten core genes (, , , , , , , , , and ) were identified, with expression not only discriminates tumor from normal tissue but also separates KIRC from KICH/KIRP, proposing as a potential second-step biomarker for KIRC identification on top of traditional histology. Inter-subtype RCC heterogeneity represented a key factor limiting predictive performance of the six prognostic signature genes (, , , , and ). Its 5-year AUC exceeded 0.7 for every individual RCC subtype in the TCGA training cohort, with pooled 5-year AUCs of 0.61 (TCGA training cohort) and 0.67 (independent PCAWG validation cohort). The prognostic risk model demonstrated strong predictive performance, with a C-index of 0.791 before calibration and 0.774 after calibration. Importantly, the C-index remained above 0.75 throughout the 60-month follow-up period, indicating stable and robust long-term prognostic accuracy. High-risk patients exhibited greater immune cell infiltration, indicating potential for immunotherapy. Following secondary screening, three RCC cell lines (BFTC909, CAKI1, and CAL54) and five target genes (, , , and ) were identified as optimal candidates for subsequent mechanistic investigations. This study uncovers the prognostic and functional relevance of SUMOylation in RCC and offers a novel framework for biomarker development, therapeutic targeting, and immunotherapeutic stratification. - Source: PubMed
Publication date: 2026/07/23
Zhang XiaoboLi ZhimingLin RuoxinYang SupingSun XiaohuiChen Shicheng - Milk oligosaccharides (MOs) play a crucial role in the development of the epithelial barrier function in newborn mammals. - Source: PubMed
Publication date: 2026/08/18
Xie KunhongXu YedanYu BingHuang ZhiqingLuo YuhengZheng PingMao XiangbingYu JieLuo JunqiuYan HuiHe Jun - - Source: PubMed
Akal CanHaskoloğlu Zehra Şuleİslamoğlu CandanSüleyman MerveAcar Mustafa OğuzKutlay Nüket YürürDoğu Esin Figenİkincioğulları Kamile Aydan - This experiment was conducted to investigate the effects of mulberry leaf powder (MLP) on alleviating fatty liver hemorrhagic syndrome (FLHS) in laying hens. A total of 180 healthy 33-week-old Hy-Line Gray laying hens were randomly assigned to 3 groups, with 6 replicates per group and 10 chickens per replicate, and fed a basal diet (CON), high-energy-low-protein (HELP) diet (FLHS), and HELP + 5% MLP diet (MLP), respectively. The experiment lasted 56 days. Results showed that the HELP diet successfully induced FLHS, characterized by an increased liver index, hepatic steatosis, and oxidative stress (P < 0.05). MLP significantly alleviated FLHS-induced hepatic steatosis, and enhanced antioxidant capacity (superoxide dismutase, catalase, glutathione peroxidase, total antioxidant capacity, and glutathione) in serum and liver (P < 0.05). Transcriptomic analysis identified 102 differentially expressed genes, whose expression was increased by FLHS but reversed by MLP, including SREBF1, SLC27A4, PLIN2, ACOT13, APOA4, GLRX, and MSRB1, which mainly participate in lipid droplet dynamics and redox homeostasis. qRT-PCR confirmed that upregulated expression of lipogenic genes (such as FASN, ACACA, SCD, and ELOVL6) and the downregulated expression of oxidative stress-responsive genes (SOD1 and GPX1) in the FLHS group were normalized by MLP (P < 0.05), accompanied by increased expression of PPARα, CPT1A, and CD36 (P<0.05). 16S rRNA sequencing revealed that MLP reduced the abundance of Desulfovibrio, Rikenellaceae_RC9_gut_group, Intestinimonas, and Shuttleworthia, and enriched Faecalibacterium and Bifidobacterium in the cecum of FLHS laying hens (P < 0.05). Furthermore, MLP increased acetate, propionate, and butyrate levels, and decreased isobutyrate and isovalerate levels (P < 0.05). Correlation analysis showed that cecal acetate, propionate, butyrate, Faecalibacterium and Bifidobacterium were positively correlated with hepatic antioxidant enzyme activities (P < 0.05), and negatively correlated with hepatic lipogenic gene expression, hepatic lipid deposition and oxidative stress markers (P < 0.05). These findings indicated that MLP alleviated hepatic lipid metabolism disorders and oxidative stress in FLHS laying hens, which might be achieved by modulating gut microbiota structure and short-chain fatty acid production. - Source: PubMed
Publication date: 2026/07/16
Xuan LingLiu HongliZhang HengGao QingtaoShang YanLiu XuelanYan PeipeiShi TianhongFu Chunyan