Ask about this productRelated genes to: ST6GALNAC6 Blocking Peptide
- Gene:
- ST6GALNAC6 NIH gene
- Name:
- ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
- Previous symbol:
- SIAT7F
- Synonyms:
- ST6GALNACVI
- Chromosome:
- 9q34.11
- Locus Type:
- gene with protein product
- Date approved:
- 2004-05-11
- Date modifiied:
- 2016-10-05
Related products to: ST6GALNAC6 Blocking Peptide
Related articles to: ST6GALNAC6 Blocking Peptide
- Ulcerative colitis (UC) involves chronic intestinal inflammation, epithelial barrier disruption, and microbial dysbiosis. Theabrownin (TB), a major bioactive compound derived from Pu-erh tea, has demonstrated anti-inflammatory properties, yet its role in intestinal barrier protection and underlying mechanisms remain unclear. In this study, TB (1 g per kg BW) was administered to dextran sulfate sodium salt (DSS)-induced colitis mice, leading to a marked alleviation in body weight loss and colon injury. TB significantly enhanced goblet cell function and mucus layer integrity, thereby supporting microbial homeostasis and epithelial defense. 16S rDNA sequencing revealed that TB restored microbial balance by enriching Prevotellaceae_UCG-001 while suppressing Eubacterium and Parasutterella. Mechanistically, TB upregulated ST6GalNAc6, reduced sialyl-Tn (STn) expression, and normalized MUC2 sialylation; inhibition of ST6GalNAc6 with 3Fax-Neu5Ac abrogated these protective effects. Collectively, these findings demonstrate that TB enhances intestinal barrier integrity and microbiota balance through modulation of the ST6GalNAc6-STn-MUC2 axis, highlighting its potential as a functional food ingredient for gut health management. - Source: PubMed
Publication date: 2026/04/27
Xie KeqinQin YumeiLi LuluZhu QingYe TongWang WenzhuHan JianzhongGu Yanpei - Successful pregnancy requires precise immune interactions between fetal extravillous trophoblasts (EVT) and maternal decidual immune cells at the maternal-fetal interface. Glycosylation, particularly terminal sialylation, is emerging as a key modulator of these interactions; however, its functional role in regulating the EVT-immune crosstalk remains poorly defined. Here, we aimed to identify a critical sialic acid-Siglec-7-IL-8-STAT3 signaling axis that promotes EVT invasiveness and is disrupted during recurrent pregnancy loss (RPL). Using primary human tissues and organ-on-chip models, we demonstrate that EVTs from patients with RPL exhibit reduced sialylation, coinciding with an increased proportion of Siglec-7⁺ decidual natural killer (dNK) cells. Mechanistically, sialylated glycoproteins on EVT surfaces engage Siglec-7, stimulating IL-8 secretion by dNK cells, which, in turn, activates STAT3 in EVTs to enhance migration and invasion. Restoration of EVT sialylation re-engages Siglec-7, rescues IL-8-STAT3 signaling, and restores invasive capacity. Our findings reveal that defective EVT sialylation disrupts a key immunological checkpoint that normally promotes EVT invasion and potentially contributes to RPL. This work provides direct mechanistic evidence that specific glycan-encoded immune signals at the maternal-fetal interface are critical for healthy pregnancy outcomes and suggests that modulating sialylation may offer a therapeutic strategy for RPL. Proposed model of sialic acid-Siglec-7-mediated regulation of EVT invasion through the ST6GALNAC6-sialic acid-Siglec-7-IL-8-STAT3 signaling axis. Schematic representation of the working model: enhanced sialylation of EVT membrane glycoproteins-driven by ST6GALNAC6-facilitates recognition by Siglec-7 expressed on dNK cells. This interaction promotes the activation of the IL-8-STAT3 signaling pathway, which supports EVT cell migration and invasion. Disruption of sialylation or Siglec-7 engagement impairs this pathway and reduces EVT invasiveness, potentially contributing to the pathogenesis of RPL. Figure created with BioRender.com ( https://BioRender.com/dxxt5az ). - Source: PubMed
Publication date: 2026/03/02
Zhang LinyuFeng YingWu PengChen LiuyanJiang NanMa XueMa QianhongLu Hao-JieXiao XueMa Fang - The immunosuppressive tumor microenvironment reduces immune response effectiveness in stromal-rich tumors, including consensus molecular subtype 4 colorectal cancer (CRC). Mesenchymal stromal cells (MSCs), precursors to cancer-associated fibroblasts (CAFs), promote cancer progression by suppressing anti-tumor immune responses. Hypersialylation of glycans on tumors engages Siglec receptors on immune cells, driving immune dysfunction, but its role in stromal-mediated suppression of innate immunity remains unclear. - Source: PubMed
Publication date: 2025/10/19
O'Neill AoiseZakaria NorashikinBull CourtneyEgan HannahCorry Shania MLeonard Niamh AO'Meara ClodaghHoward LindaWalsh AnastasijaReidy EileenChe JennyPeng LiCao LizhiEgan Laurence JRitter ThomasSheehan MargaretCanney AoifeCulligan KevinHogan Aisling MHynes Sean ODunne Philip DO'Dwyer MichaelTreacy OliverRyan Aideen E - The two sialyltransferases in the ST6GALNAC subfamily (EC 2.4.99.-; CAZy family GT29), ST6GalNAc5 and ST6GalNAc6, catalyze the formation of the linkage from the sialic acid moiety to the C6 position of N-acetylgalactosamine (GalNAc) as well as to N-acetylglucosamine (GlcNAc), and are known as α-2,6-sialyltransferases. This activity is interesting for the synthesis of the disialylated oligosaccharide disialyllacto-N-tetraose (DSLNT). Human sialyltransferases ST6GalNAc5 and ST6GalNAc6 produced in HEK293 cells are commercially available at a smaller scale. In this study, we demonstrated that ST6GalNAc5 and ST6GalNAc6 can be functionally expressed in Pichia pastoris X-33. The level of ST6GalNAc5 and ST6GalNAc6 expression and activity largely depended on the type of construct, as well as on expression conditions, namely temperature, methanol feeding regime, and supplements. Insertion of a (GGGS)₂ linker peptide between the gene and the α secretion factor improved the secretion of active enzyme in P. pastoris X-33. The use of media supplemented with MgCl and Casamino acids led to increased cell growth and, importantly, enhanced ST6GalNAc5 and ST6GalNAc6 production. Under optimized conditions, the P. pastoris X-33 strain could secrete up to 10 mg of active sialyltransferase protein per liter of culture. Compared to their wild-type counterparts, mutants of ST6GalNAc5 and ST6GalNAc6 devoid of N-glycosylation sites exhibited reduced enzymatic activity and stability. Apart from contributing to successful P. pastoris expression, our findings also contribute to a deeper understanding of the role of N-glycosylation in the activity and stability of sialyltransferases. KEY POINTS: • Expression of functional human ST6GalNAc5 and ST6GalNAc6 in Pichia pastoris • Mutants devoid of N-glycosylations lack activity • Media supplementation with MgCl2 and Casamino acids improves expression. - Source: PubMed
Publication date: 2025/10/15
Krasnoselska GannaLengyel MartonMatwiejuk MartinVuillemin MarleneMolnar-Gabor DoraMeyer Anne SZeuner Birgitte - Sialic acids (Sias) are ubiquitously expressed on all types of glycans, typically as terminating residues. They usually link to galactose, N-acetylgalactosamine, or other Sia residues, forming ligands of many glycan-binding proteins. An atypical linkage to the C6 of N-acetylglucosamine (GlcNAc) has been identified in human milk oligosaccharides (HMOs, e.g., DSLNT) and tumor-associated glycoconjugates. Herein, describe the systematic synthesis of these HMOs in an enzymatic modular manner. The synthetic strategy relies on a novel activity of ST6GalNAc6 for efficient construction of the Neu5Acα2-6GlcNAc linkage, and another 12 specific enzyme modules for sequential HMO assembly. The structures enabled comprehensive exploration of their structure-function relationships using glycan microarrays, revealing broad yet distinct recognition by Siglecs of the atypical Neu5Acα2-6GlcNAc motif. The work provides tools and new insight for the functional study and potential applications of Siglecs and HMOs. - Source: PubMed
Publication date: 2024/10/24
Bao ShuminShen TangliangShabahang Mohammad HosseinBai GuitaoLi Lei