Ask about this productRelated genes to: RPL10A Blocking Peptide
- Gene:
- RPL10A NIH gene
- Name:
- ribosomal protein L10a
- Previous symbol:
- NEDD6
- Synonyms:
- Csa-19, L10A
- Chromosome:
- 6p21.31
- Locus Type:
- gene with protein product
- Date approved:
- 1994-01-21
- Date modifiied:
- 2015-09-07
Related products to: RPL10A Blocking Peptide
Related articles to: RPL10A Blocking Peptide
- Astrocytes play essential roles in maintaining brain homeostasis and in contributing to synaptic functions, but, in response to injury, infection, or disease, astrocytes can downregulate their homeostatic and physiological functions while increasing neuroinflammatory responses. The central amygdala (CeA) is important for stress responsivity and the development of alcohol (ethanol) dependence. Using a multi-omics approach in Aldh1l1-EGFP/Rpl10a mice and the chronic intermittent ethanol two-bottle choice (CIE-2BC) model, we have characterized the translational response of CeA astrocytes, as well as the proteomic and phosphoproteomic changes in ethanol dependent, non-dependent, and naïve mice. We identified astrocyte-specific alterations in neuroimmune functions and antioxidant/oxidative stress pathways in ethanol dependent mice as well as cytoskeletal plasticity related pathways in non-dependent mice. Proteomic analysis showed down-regulation of astrocyte physiological functions in dependent animals while phosphoproteomic analysis identified pathways associated with cytoskeleton remodeling in both dependent and non-dependent mice. Reconstructions of astrocyte morphologies demonstrated increased CeA astrocyte complexity in dependent and non-dependent groups compared to naïve mice. The astrocyte-specific activation of neuroimmune and antioxidant pathways, down-regulation of homeostatic functions, alteration in protein phosphorylation-mediated cytoskeleton remodeling, and increased astrocyte morphological complexity demonstrate that ethanol dependence induces astrocyte reactivity in the CeA consistent with both adaptive and maladaptive changes. These findings highlight the role of CeA astrocytes in the progression from alcohol intake to dependence and represent a first step toward identifying astrocyte-specific therapeutic strategies to treat Alcohol Use Disorder (AUD) aimed at potentiating reactive astrocyte adaptive changes and inhibiting maladaptive responses. - Source: PubMed
Publication date: 2026/04/06
Hashimoto Joel GGonzalez Angela EGorham NatalieBarbour ZoeRoberts Amanda JDay Le ZNedelescu HerminaHeal MaciDavis Brett ACarbone LuciaJacobs JonRoberto MarisaGuizzetti Marina - Pronuclear Envelope Breakdown (PNEB) failure is a critical factor contributing to the early developmental arrest of intracytoplasmic sperm injection (ICSI) embryos; however, its molecular mechanism remains inadequately understood. This study aimed to elucidate the core regulatory network underlying PNEB failure occurrence and its impact on embryonic development. Single-cell sequencing was used to identify differentially expressed genes (DEGs) between PNEB‑type two pronuclei (2PN) zygotes and 3PN control zygotes, and weighted gene coexpression network analysis (WGCNA) was performed to identify module genes associated with PNEB failure. The least absolute shrinkage and selection operator was applied to model disease types and screen core genes, thereby establishing a multiomics integration strategy. Reactive oxygen species (ROS) and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide (JC-1) fluorescence staining were conducted to analyze oxidative stress and mitochondrial function. A total of 1294 DEGs were identified, including genes involved in the oxidative phosphorylation pathway. Mitochondrially encoded reduced nicotinamide adenine dinucleotide dehydrogenase 1 (MT-ND1) and mitochondrially encoded cytochrome c oxidase III (MT-CO3) were significantly upregulated, whereas genes associated with the DNA repair pathway were downregulated. WGCNA revealed a light-green module strongly associated with PNEB failure (r = 0.89, P = 1.2e - 5). Hub genes, ribosomal protein L10a (RPL10A) and ribosomal protein L38 (RPL38), within this module were implicated in ribosome biogenesis. The PPI network confirmed functional interactions between MT-ND1 and RPL10A, suggesting that dysregulated mitochondrial function and ribosomal assembly are central to PNEB failure. ROS and JC-1 fluorescence staining showed a significant decrease in the JC-1 red/green fluorescence intensity ratio in the PNEB failure group (p < 0.05), while ROS levels were significantly elevated (p < 0.01). This study reveals that mitochondrial metabolic dysfunction and ribosomal assembly abnormalities contribute to PNEB failure, thereby disrupting nuclear envelope stability. Furthermore, it identifies the MT-ND1-RPL10A/RPL38 axis as a potential novel molecular marker for assessing ICSI embryo quality. - Source: PubMed
Wang ChaoyingFang JunnanYang GuangJiang RanJin HaixiaSong WenyanShi SenlinZhai JunWang HuihuiZhang TongweiYao Guidong - Xizang sheep are vital economic livestock in plateau regions. Traditional surgical castration often induces stress and infection. Although immunization presents an alternative method, the physiological mechanisms underlying its effects in Xizang sheep remain unclear. Therefore, this study integrated serum immune-antioxidant indicators with hypothalamus-pituitary transcriptomics to investigate molecular mechanisms of GnRH immunization. Results indicated that serum IgA, IgG, IgM, SOD, and GSH in the immunization (IM) group were significantly higher than in control (CON) and surgical castration (SN) groups, while IL-6 and TNF-α were significantly reduced ( < 0.05). RNA-seq analysis revealed that hypothalamic , , , , and were significantly upregulated in IM group, whereas and were significantly downregulated, with notable enrichment in prion disease, oxidative phosphorylation, and thermogenesis pathways. Pituitary was upregulated while , , , , and were significantly downregulated, showing enrichment in myofibril, contractile fiber, sarcomere, and cytosolic ribosome pathways. Association analysis revealed significant positive correlations between IgG and pituitary , , , as well as hypothalamic , , and . In summary, GnRH immunization outperforms surgical castration by modulating hypothalamic-pituitary genes and enhancing immunity and antioxidants in plateau Xizang sheep, achieving integrated neuroendocrine-immune regulation for healthy husbandry of plateau Xizang sheep. - Source: PubMed
Publication date: 2026/02/19
Song TianzengMustafa Shehr BanoLi HaiyanZhang XiaomingWang GaofuZhang TingtingChen XiaoyingCui JianzhaoZhang MingZeng XianyinLiu GuiqiongXian LiliJiayang ZhuomaZhao WangshengJiang Xunping - Alcohol Use Disorder is a leading preventable cause of morbidity and mortality, yet knowledge of mechanisms driving ethanol-related neuroplasticity remains incomplete. While research has traditionally focused on neuronal signaling, emerging evidence implicates astrocytes in addiction-related adaptations. Here, we investigated the astrocyte-specific molecular consequences of chronic ethanol consumption in the prefrontal cortex and nucleus accumbens, two brain regions critical for executive control and reward processing. Using Translating Ribosome Affinity Purification RNA-seq and bulk RNA-seq in Aldh1l1-EGFP/Rpl10a mice, expressing an EGFP tag on astrocyte ribosomes, we identified hundreds of differentially translated astrocytic genes following chronic continuous two-bottle choice ethanol drinking. Sex-specific analyses revealed a higher number of astrocytic changes in the female PFC and male NAc. Pathway enrichment highlighted extracellular matrix remodeling, synaptic signaling, mitochondrial function, and immune-related pathways. Analyses of individual drinking levels further demonstrated distinct correlations between ethanol intake and astrocytic translation. The major components of the brain extracellular matrix are chondroitin sulfate proteoglycans, produced primarily by astrocytes and covalently bound to chondroitin sulfate glycosaminoglycan chains. Complementary mass spectrometry/liquid chromatography analyses of chondroitin sulfate, heparan sulfate, and hyaluronic acid glycosaminoglycan disaccharides revealed ethanol-induced alterations in chondroitin sulfate glycosaminoglycan sulfation patterns, with additional baseline differences identified between selectively bred high- and low-ethanol preference lines. Together, these findings indicate that astrocytes undergo profound sex- and region-specific adaptations to chronic ethanol, implicating extracellular matrix and glycosaminoglycan remodeling as key risk-factors for and mediators of chronic ethanol-related neuroplasticity. - Source: PubMed
Publication date: 2026/02/17
Hashimoto Joel GOzburn Angela RReed CherylErk JasonSong YuefanYang JiyuanXia KeZhang FumingYu YunFei Suzanne SGao LinaLinhardt Robert JPhillips Tamara JGuizzetti Marina - Zebrafish are a powerful model for investigating vascular and lymphatic biology due to their genetic tractability and optical transparency. While translating ribosome affinity purification (TRAP) has been widely applied in other systems, its application in zebrafish has remained limited. Here, we present an optimized TRAP protocol for isolating ribosome-associated mRNAs from endothelial cells in vivo, without the need for cell dissociation or sorting. Using a novel transgenic zebrafish line, which expresses HA-tagged Rpl10a under the promoter, we enriched actively translating endothelial transcripts. Differential expression analysis revealed robust upregulation of vascular and lymphatic genes including , and . This approach captures the endothelial cell translatome with high specificity and offers a robust platform for investigating the molecular mechanisms of endothelial biology under genetic, environmental, or toxicological perturbations. Key features • Permits in vivo isolation of the endothelial ribosome without cell sorting. • Optimized TRAP protocol to analyze lymphatic and venous endothelial gene expression in zebrafish. • Utilizes a stable transgenic zebrafish line. • Compatible with real-time quantitative PCR (qPCR) and next-generation sequencing (RNA-seq). - Source: PubMed
Publication date: 2025/12/05
Olayinka OlamideZarinebaf LeiaJung Hyun Min