Ask about this productRelated genes to: DPY19L1 Blocking Peptide
- Gene:
- DPY19L1 NIH gene
- Name:
- dpy-19 like C-mannosyltransferase 1
- Previous symbol:
- -
- Synonyms:
- KIAA0877
- Chromosome:
- 7p14.2
- Locus Type:
- gene with protein product
- Date approved:
- 2005-07-19
- Date modifiied:
- 2017-08-04
Related products to: DPY19L1 Blocking Peptide
Related articles to: DPY19L1 Blocking Peptide
- C-Mannosyl tryptophan (C-Man-Trp), a unique monomeric glycosyl amino acid, is up-regulated in the blood of ovarian cancer patients; however, the underlying mechanisms remain unclear. In the present study, C-Man-Trp production and its dynamics were investigated in female B6C3F1 mice transplanted with mouse ovarian cancer OV2944-HM-1 (HM-1) cells. After transplantation, C-Man-Trp levels increased in the plasma, urine, ascites, peritoneal exudate cells (PECs), and tumor masses of mice. Furthermore, changes in the transcriptional expression of C-Man-Trp metabolism-related genes, C-mannosyltransferases (Dpy19l1 and Dpy19l3), and thrombospondin type I repeat superfamily genes (Thbs1, Spon1, and cellular communication network factor 1) were noted in tumor-associated cells and tissues. A cell-sorting analysis revealed that PECs mainly comprised myeloid-derived immune cells, such as macrophages and myeloid-derived suppressor cells, in addition to a small population of HM-1 tumor cells. C-Man-Trp levels were high in the macrophage fraction, but lower in the myeloid-derived suppressor cell fraction. C-Man-Trp was also produced in the ex vivo culture medium of macrophages isolated from PECs. Under macrophage depletion using clodronate liposomes, the ovarian cancer-stimulated up-regulation of C-Man-Trp was significantly suppressed in the plasma, ascites, PECs, and tumor masses of HM-1 cell-transplanted mice. C-Man-Trp levels in the plasma and peritoneal cavity cells of normal healthy mice were also suppressed by clodronate liposomes, whereas the expression of C-Man-Trp metabolism-related genes showed different changes from those in mice transplanted with HM-1 cells. Collectively, these results demonstrate that tumor-stimulated macrophages play a pivotal role in the dynamics of C-Man-Trp in mice with ovarian cancer. - Source: PubMed
Publication date: 2026/03/09
Inai YokoMinakata ShihoTsujimoto KayaManabe ShinoIwahashi NaoyukiKamijo RyotaNakadaira YumaNishikawa KeisukeHashizume TomohiroIno KazuhikoIhara Yoshito - Artificial and natural selection for important economic traits and genetic adaptation of the populations to specific environments have led to the changes on the sheep genome. Recent advances in genome sequencing methods have made it possible to use comparative genomics tools to identify genes under selection for traits of economic interest in domestic animals. - Source: PubMed
Publication date: 2022/01/11
Rezvannejad ElhamAsadollahpour Nanaei HojjatEsmailizadeh Ali - Epigenetic mechanisms may contribute to idiopathic scoliosis (IS). We identified 8 monozygotic twin pairs with IS, 6 discordant (Cobb angle difference > 10°) and 2 concordant (Cobb angle difference ≤ 2°). Genome-wide methylation in blood was measured with the Infinium HumanMethylation EPIC Beadchip. We tested for differences in methylation and methylation variability between discordant twins and tested the association between methylation and curve severity in all twins. Differentially methylated region (DMR) analyses identified gene promoter regions. Methylation at cg12959265 (chr. 7 ) was less variable in cases (false discovery rate (FDR) = 0.0791). We identified four probes (false discovery rate, FDR < 0.10); cg02477677 (chr. 17, gene), cg12922161 (chr. 2 gene), cg08826461 (chr. 2), and cg16382077 (chr. 7) associated with curve severity. We identified 57 DMRs where hyper- or hypo-methylation was consistent across the region and 28 DMRs with a consistent association with curve severity. Among DMRs, 21 were correlated with bone methylation. Prioritization of regions based on methylation concordance in bone identified promoter regions for (WNT signaling), (regulator of bone and energy homeostasis), and others predicted to be relevant for bone formation/remodeling. These regions may aid in understanding the complex interplay between genetics, environment, and IS. - Source: PubMed
Publication date: 2021/07/30
Carry Patrick MTerhune Elizabeth ATrahan George DVanderlinden Lauren AWethey Cambria IEbrahimi ParvanehMcGuigan FionaÅkesson KristinaHadley-Miller Nancy - C-mannosylation is a modification of tryptophan residues with a single mannose and can affect protein folding, secretion, and/or function. To date, only a few proteins have been demonstrated to be C-mannosylated, and studies that globally assess protein C-mannosylation are scarce. To interrogate the C-mannosylome of human induced pluripotent stem cells, we compared the secretomes of CRISPR-Cas9 mutants lacking either the C-mannosyltransferase DPY19L1 or DPY19L3 to WT human induced pluripotent stem cells using MS-based quantitative proteomics. The secretion of numerous proteins was reduced in these mutants, including that of A Disintegrin And Metalloproteinase with ThromboSpondin Motifs 16 (ADAMTS16), an extracellular protease that was previously reported to be essential for optic fissure fusion in zebrafish eye development. To test the functional relevance of this observation, we targeted dpy19l1 or dpy19l3 in embryos of the Japanese rice fish medaka (Oryzias latipes) by CRISPR-Cas9. We observed that targeting of dpy19l3 partially caused defects in optic fissure fusion, called coloboma. We further showed in a cellular model that DPY19L1 and DPY19L3 mediate C-mannosylation of a recombinantly expressed thrombospondin type 1 repeat of ADAMTS16 and thereby support its secretion. Taken together, our findings imply that DPY19L3-mediated C-mannosylation is involved in eye development by assisting secretion of the extracellular protease ADAMTS16. - Source: PubMed
Publication date: 2021/05/08
Cirksena KarstenHütte Hermann JShcherbakova AleksandraThumberger ThomasSakson RomanWeiss StefanJensen Lars RiffFriedrich AlinaTodt DanielKuss Andreas WRuppert ThomasWittbrodt JoachimBakker HansBuettner Falk F R - Lung adenocarcinoma (LUAD) is the most common histological subtype of lung cancer worldwide. Until now, the molecular mechanisms underlying LUAD progression have not been fully explained. This study aimed to construct a competing endogenous RNA (ceRNA) network to predict the progression in LUAD. - Source: PubMed
Yang DanHe YangWu BoLiu RuxiWang NanWang TietingLuo YannanLi YundaLiu Yang