LOC201725 Blocking Peptide
- Known as:
- LOC201725 Blocking Peptide
- Catalog number:
- 33r-9811
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- LOC201725 Blocking Peptide
Related genes to: LOC201725 Blocking Peptide
- Gene:
- C4orf46 NIH gene
- Name:
- chromosome 4 open reading frame 46
- Previous symbol:
- -
- Synonyms:
- LOC201725, RCDG1
- Chromosome:
- 4q32.1
- Locus Type:
- gene with protein product
- Date approved:
- 2008-07-14
- Date modifiied:
- 2016-11-22
Related products to: LOC201725 Blocking Peptide
Related articles to: LOC201725 Blocking Peptide
- Several genes have been reported to be involved in spermatogenesis but their functional importance in male fertility is yet needed to be elucidated. Therefore, in current research, we focused to explore the in vivo role of evolutionary conserved and testis-specifically expressed, C4orf46, gene in male mouse fertility and spermatogenesis. The expression profile of C4orf46 is specific to testes and expressed in testes from 7 days of postpartum to onward. Thus, we generated the C4orf46 knockout mice by utilizing CRISPR/Cas9 genome editing technology and examined gene function in spermatogenesis and fertility. Surprisingly, C4orf46 knockout mice were completely fertile, displayed normal testes morphology, however, higher sperm contents were observed in knockout mice compared to wild type (WT) littermates. Subsequently, intact testis histology and architecture of seminiferous tubules were observed in C4orf46 knockout and WT mice. Similarly, sperm morphology and swimming velocity of C4orf46 knockout mice were comparable with the WT littermates. Furthermore, all type of germ cells ranging from spermatogonia to mature spermatozoa were observed in the testes and epididymis sections of C4orf46 knockout mice suggesting that disruption of C4orf46 did not impact spermatogenesis. Moreover, meiotic prophase I progression was normal, and each type of cell population was comparable between knockout and WT mice. Overall, finding from this research indicates that C4orf46 is not an essential gene for fertility in mice. This study will help researchers to avoid the repetition and duplication of efforts, and to explore the genes that are indispensable for spermatogenesis and male fertility. - Source: PubMed
Publication date: 2021/06/02
Shah BasitKhan RanjhaShah WasimAftab AyeshaKhan MananDil SobiaShi Qinghua - Recently identified molecular tumor markers have numerous potential applications in the diagnosis, therapy and prognostic prediction of renal cell carcinoma (RCC). Through bioinformatics‑based screening approaches together with validation of western blot and immunohistochemical data, the present study identified a novel renal cancer‑associated gene, preliminarily named Renal Cancer Differentiation Gene 1 (RCDG1), originally known as chromosome 4 open reading frame 46 (C4orf46). RCDG1 expression was evaluated by western blot analysis of RCC and adjacent normal tissues, renal cancer cell lines and normal kidney HEK293T cells. Additionally, RCDG1 expression was assessed in 124 RCC paraffin sections, including 92 paired adjacent normal tissues, by immunohistochemistry. The results showed that RCDG1 was significantly downregulated in RCC tissues as compared with normal adjacent tissues (P<0.001), and the expression of RCDG1 in clear cell (cc) RCC tissues was significantly lower as compared with that of non‑ccRCC tissues (P=0.005). Furthermore, statistical analysis revealed RCDG1 expression was negatively correlated with the Fuhrman grade in ccRCC (P=0.008). A reduction in RCDG1 expression may be associated with the oncogenesis of RCC and the differentiation of ccRCC. Further studies may provide more information about the function of RCDG1 gene in RCC. - Source: PubMed
Publication date: 2014/07/16
Yu ZuhuNi LiangchaoChen DuqunSu ZhengmingYu WenshuiZhang QiangWang YadongLi CailingGui YaotingLai Yongqing