Ask about this productRelated genes to: PRKAA1 Blocking Peptide
- Gene:
- PRKAA1 NIH gene
- Name:
- protein kinase AMP-activated catalytic subunit alpha 1
- Previous symbol:
- -
- Synonyms:
- AMPKa1
- Chromosome:
- 5p13.1
- Locus Type:
- gene with protein product
- Date approved:
- 1997-05-09
- Date modifiied:
- 2016-01-27
Related products to: PRKAA1 Blocking Peptide
Related articles to: PRKAA1 Blocking Peptide
- Primary Ovarian Insufficiency (POI) is a highly heterogeneous condition characterized by the cessation of ovarian function before age 40. While genetic factors play a substantial role, the contribution of structural variants remains incompletely mapped. We conducted a systematic review and in silico genomic re-analysis of published copy number variations (CNVs) in individuals with POI. Following PRISMA guidelines, we aggregated 382 CNVs from 25 studies, standardized genomic coordinates, and filtered variants against population databases. Pathogenicity was re-evaluated using ACMG/ClinGen guidelines, yielding 42 pathogenic/likely pathogenic variants and 25 large CNVs (>3.5 Mb). Consistent with previous findings, the X chromosome exhibited the highest CNV burden, emphasizing its central role in structural genomic instability and POI pathogenesis. Beyond canonical POI-associated genes, gene ontology and GTEx expression profiling identified several biologically plausible, highly ovary-expressed candidate genes within disrupted loci-notably ATF3, GAS5, PPP4R1, and PRKAA1. Despite their established roles in cellular stress responses, DNA repair, and meiotic progression, these genes remain absent from most commercial POI diagnostic panels. This comprehensive re-analysis highlights the complex structural genomic landscape of POI and suggests that expanding current clinical testing panels to include these under-recognized genes could improve diagnostic yields for genetically unexplained cases. - Source: PubMed
Publication date: 2026/05/13
Hossein Garakani MelikaKakavand KianoushHajiesmaeili YasamanHaratian KavehMoradi Mahtab ZZarei Moradi ShabnamMohseni Meybodi Anahita - Hyperlipidaemia (HLP) arises from impaired lipid homeostasis in the setting of chronic low-grade inflammation, yet mechanistic comparisons between the edible medicinal fungi Cordyceps militaris and Ophiocordyceps sinensis remain scarce. Here, we combined chemical profiling with target/pathway prioritisation and structure-based modelling to define shared and species-specific lipid-regulatory features of these two fungi, followed by in vivo validation of cordycepin, a representative component of C. militaris, in high-fat diet (HFD)-fed mice. Integrated network analysis identified 72 common HLP-related targets, supporting convergence on inflammation-metabolism crosstalk. C. militaris displayed a more concentrated signature, characterised by GRIK family and proteasome-associated hubs and prominent enrichment of AMPK-related signalling. In contrast, O. sinensis was preferentially associated with upstream regulatory networks including insulin signalling, PI3K-Akt, MAPK and HIF-1. Molecular dynamics simulations showed relatively stable behaviour for the GRIK5-cordycepin and HCAR2-nicotinic acid complexes, whereas ERBB2-cerevisterol exhibited larger conformational fluctuation. MM-PBSA calculations further provided quantitative support for ligand-target association in the selected representative complexes. HPLC confirmed cordycepin as a characteristic component of C. militaris. In vivo, cordycepin improved fasting glucose and circulating lipid profiles, alleviated hepatic steatosis-like changes, and was accompanied by increased hepatic Prkaa1 and decreased Srebf1 expression. Collectively, these findings provide comparative computational insights into the hypolipidaemic potential of C. militaris and O. sinensis, while supporting cordycepin as a bioactive constituent of C. militaris associated with transcriptional changes related to AMPK/SREBP-1c signalling. The predicted regulatory features of O. sinensis still require direct experimental validation. - Source: PubMed
Publication date: 2026/05/09
Shi HuaijieZhang GuoyingLing Jianya - Secondary bile acids (SBAs) are increasingly recognized as hormone-like signals in vertebrates, yet their regulatory potential in fish adipose tissue remains largely unexplored. This study evaluated the acute effects of SBAs (500 µM lithocholic acid -LCA-, 1500 µM deoxycholic acid -DCA-, and their taurine conjugates: 1000 µM T-LCA and 600 µM T-DCA) on the transcriptomic profile, glucose and lipid homeostasis, and bile acid (BA)-related pathways in rainbow trout white adipose tissue (WAT) six hours after intragastric administration. The main results showed that LCA emerged as the main metabolic modulator, enhancing glycolytic entry points via increased hexokinase and pyruvate kinase activities, modulating energy-sensing genes prkaa1/2 (Ampk) and mtor (mTOR), and promoting coordinated adjustments in lipid metabolism, including upregulation of acly and hadh (Hoad), increased Acly activity, and reduced NEFA levels suggesting a potential enhancement of metabolic flexibility and intracellular lipid turnover. In contrast, DCA induced a more restricted response, upregulating mRNA abundance of genes encoding the BA transporters Ostα1 and Ostβ, the receptor FXRα-1, and Cyp8b1 isoforms, suggesting a targeted role in local BA sensing and signaling. Taurine-conjugated SBAs generally produced attenuated effects, with T-DCA tending to promote lipid retention and T-LCA evoking minimal changes. Overall, this study provides the first evidence of BA-related elements in rainbow trout WAT and suggests that SBAs can potentially modulate adipose tissue function in a manner dependent on BA identity, laying the foundation for future studies on their physiological and nutritional roles. - Source: PubMed
Publication date: 2026/05/09
Pérez-Tierra GabrielCalo JessicaBlanco Ayelén MSoengas José L - AMP-activated protein kinase (AMPK) serves as a crucial energy sensor, maintaining organismal energy homeostasis through the regulation of diverse metabolic pathways. However, the association between the AMPK signaling pathway and hypoxia adaptation in yak lung tissue has not yet been elucidated. Consequently, this study focused on the lung tissues of yaks and cattle residing at the same altitude (2600 m). Morphological analysis demonstrated that, compared to cattle, yak lung tissue possessed significantly thicker alveolar septa (P < 0.05), a greater abundance of elastic fibers (P < 0.05), and a reduced blood-air barrier thickness (P < 0.05), suggesting substantial structural adaptations in the yak lung under identical altitudinal conditions. RNA-seq analysis identified 3684 genes with significant differential expression between yaks and cattle. KEGG pathway enrichment analysis showed significant enrichment for the AMPK signaling pathway under the "Environmental Information Processing" category, and Gene Set Enrichment Analysis (GSEA) further confirmed the activation of the AMPK signaling pathway in yak lung tissue. Despite qRT-PCR indicating reduced mRNA levels of key AMPK pathway genes (PRKAA1, PRKAA2, PRKAB1, PRKAG2) in yak lung tissue, Western blot analysis demonstrated a marked upregulation in the relative abundance of phosphorylated AMPK (P-AMPK α1 + α2), implying potential activation of the AMPK signaling pathway via phosphorylation in yak lung tissue. Further analysis of downstream gene expression within the AMPK signaling pathway indicated significant downregulation of genes associated with glucose metabolism (PCK2, G6PC1), lipid metabolism (FASN, ACACA), protein metabolism (MAPKAPK5, MTOR), cell proliferation and apoptosis (RPTOR, MTOR), and autophagy (TXNIP, NLRP3) in yak lung tissue. These findings suggest that, relative to cattle, the yak lung may adapt to hypoxic conditions by minimizing energy expenditure, suppressing aberrant cell proliferation, mitigating oxidative stress, and reducing inflammatory responses. In summary, the activation of the AMPK signaling pathway in yak lung tissue may play a crucial role in hypoxic adaptation by enhancing oxygen utilization and energy supply capacity. - Source: PubMed
Publication date: 2026/04/04
Zhang XunDing WeiqinWang HuizhenLi JingyiChen JiaruiWei Qing - The present study explored the effects of dietary supplementation with hot water extract of Juncao-substrate Ganoderma lucidum residue (HWE-JGLR) on growth performance, carcass traits, meat quality, and antioxidant capacity of Liancheng white ducks. A total of 288 one-day-old male Liancheng white ducks were randomly allocated into 4 groups with 6 replicates of 12 ducks each. The control group was fed a corn-soybean meal basal diet, while the test groups were fed a basal diet supplemented with either 0.25%, 0.5%, or 1% HWE-JGLR, designated as HJ-I, HJ-Ⅱ, and HJ-Ⅲ groups, respectively, for 63 days. No significant differences were observed in growth performance or carcass traits among the groups. Regarding meat quality, the primary beneficial effect was the mitigation of pH decline in breast muscles (P < 0.05); no significant effects were observed in shear force, drip loss, or meat color of both breast and leg muscles. In breast muscle, the expression levels of genes CPT-1 and PRKAA1 were elevated by HWE-JGLR supplementation (P < 0.05). In contrast, a marked suppression was observed in the expression of SREBP-1C (P < 0.05). Additionally, compared with the control group, the serum T-SOD and GSH-Px activities in ducks fed HJ-Ⅱ and HJ-Ⅲ diets were found to be increased (P < 0.05). Besides, ducks fed HJ-I and HJ-Ⅲ diets reduced serum MDA concentration (P < 0.05). In liver and breast muscle, HJ-Ⅱ and HJ-Ⅲ groups increased the expression of CAT, SOD, TXN, GCLC, NRF2 and NQO1 (P < 0.05). Meanwhile, the activity of liver GSH-Px and breast muscle CAT was also enhanced (P < 0.05). In summary, diet supplemented with HWE-JGLR has been shown to improve the meat quality of breast muscle and suppress the expression levels of fat-related genes. Meanwhile, HWE-JGLR promoted the antioxidant capacity of Liancheng through a coordinated increase in antioxidant enzyme activities and upregulation of associated gene expression. Based on the results of various measurement indicators, it is appropriate to add 0.5% HWE-JGLR in feed. - Source: PubMed
Publication date: 2026/03/18
Cai Zai-XingLv Hai-XuanYang YunGu Xiao-MingLiu Xiao-PingJin LingGao Yu-Yun