Ask about this productRelated genes to: RHCE Blocking Peptide
- Gene:
- RHCE NIH gene
- Name:
- Rh blood group CcEe antigens
- Previous symbol:
- RH
- Synonyms:
- CD240CE
- Chromosome:
- 1p36.11
- Locus Type:
- gene with protein product
- Date approved:
- 1993-10-22
- Date modifiied:
- 2019-04-23
Related products to: RHCE Blocking Peptide
Related articles to: RHCE Blocking Peptide
- The Rh blood group system includes several compound antigens, among which the f antigen (ISBT 004006) is expressed when the c and e antigens occur in cis position on the same RhCE protein. Anti-f is a rare alloantibody but is clinically significant due to its potential to cause hemolytic transfusion reactions and hemolytic disease of the newborn. We report a rare case of anti-f with coexisting anti-c identified during pre-transfusion testing in a 40-year-old female admitted for elective hysterectomy. Her blood group was O Rh D positive and her antibody screen was positive with a negative autocontrol and direct antiglobulin test. Extended phenotyping revealed CCee, K-negative phenotype. Antibody identification using an 11-cell panel suggested anti-c and anti-f, which was further confirmed by allogeneic adsorption studies using Rh-phenotyped donor cells. During cross-matching, only R1R1 units were found to be compatible. This case highlights the importance of extended Rh phenotyping, adsorption studies, and careful selection of compatible donor units in resolving rare Rh antibody combinations to ensure safe transfusions to patients. - Source: PubMed
Publication date: 2026/05/28
Kakkar BrindaBaldota RutujaGokhale AshaKetkar SanjivChavan MinakshiKeripale Amruta - For testing ocular irritation, 3D corneal models mimicking the corneal epithelium are considered reliable eye irritation tests and are detailed in regulatory guideline OECD Test Guideline (TG) 492. The aim of the present study was to develop and validate a Reconstructed human Cornea-like Epithelium (RhCE) irritation test method for ophthalmic medical devices according to OECD TG 492. Immortalized Human Primary Corneal Epithelium Cells (IM-HCEpiCs) were cultured on microporous inserts and exposed to an Air-Liquid Interface (ALI). Morphology was examined using standard (immuno-) histological techniques. Viability was quantified with MTT assay. Barrier integrity and function were monitored by trans-epithelial electrical resistance (TEER) and determination of IC using MTT assay. Reproducibility was evaluated by calculating the inter-batch coefficient of variation (CV %) of the absorbance values of negative control-treated RhCE model replicates by MTT assay. Technical proficiency was verified using reference chemicals. Irritancy of ophthalmic medical devices was assessed. IM-HCEpiCs developed an epithelium-like barrier under the ALI. TEER increased after ALI introduction, and the obtained IC value showed concordance with the guideline's reference ranges. The developed RhCE test method demonstrated technical proficiency and correctly identified medical devices as non-irritants. A novel RhCE model was developed and validated according to OECD TG 492. - Source: PubMed
Publication date: 2026/05/11
Rawat PayalRodella UmbertoD'Agostino StefaniaRagazzi EugenioRossi OriettaGatto ClaudioGiurgola LauraD'Amato Tóthová Jana - Accurate typing of the D antigen is critical to provide guidance for blood transfusion and safe management of Rh-incompatible pregnancies. RHD genotyping assays have been widely adopted to improve D variant detection, especially for common RHD alleles with missense variants and RHD-CE-D hybrids. Few structural variants of the RHD gene are known to involve large fragments of non-RH sequences, despite causing phenotype-genotype discrepancies. - Source: PubMed
Publication date: 2026/05/12
Wen JizhiHe FeiVeldhuisen BarberaMo ChunyanWei LingJia Shuangshuangvan der Schoot C EllenFlegel Willy AlbertJi Yanli - Serological classification of RhD-negative and weak D is often insufficient in admixed populations, where diverse RHD variants have clinical implications. In Brazil's highly admixed population, RhD interpretation and donor-recipient matching are particularly challenging. We used Duffy phenotyping to support ancestry inference and contextualize Rh variants. Understanding RHD alleles and their associations with RhCE and Duffy is essential for transfusion safety. Here, we characterize RHD variants in Brazilian donors and relate them to RhCE and Duffy phenotypes across regions. - Source: PubMed
Publication date: 2026/05/08
Silva Thamy C SDezan Marcia RCosta SidneiaGirardo Beatriz CCruz Bruno RZiza KarenLanghi Dante MBordin José OCastilho LilianDinardo Carla L - Detecting Rh variants in transfusion recipients is essential to prevent alloimmunization. This study compared the serological reactivity of various monoclonal antibodies using two techniques (gel column agglutination and microplate technology at room temperature) in relation to genetically confirmed Rh variants. - Source: PubMed
Publication date: 2026/05/08
Lauwers MaïlisAkiki PhilippeBensaid SamyBakrim SaadMonfort MélanieEl Kenz Hanane