Ask about this productRelated genes to: MRTO4 Blocking Peptide
- Gene:
- MRTO4 NIH gene
- Name:
- MRT4 homolog, ribosome maturation factor
- Previous symbol:
- C1orf33
- Synonyms:
- dJ657E11.4, MRT4
- Chromosome:
- 1p36.13
- Locus Type:
- gene with protein product
- Date approved:
- 2002-05-23
- Date modifiied:
- 2019-02-01
Related products to: MRTO4 Blocking Peptide
Related articles to: MRTO4 Blocking Peptide
- The present study investigated the protective effects of polysaccharides (IOPs), artemisinin, and dihydroartemisinin (DHA) on Neospora caninum-induced damage of the reproductive system in male BALB/c mice. This study conducted in vivo resistance experiments against Neosporidium using three drugs: polysaccharides from Betula brownii, artemisinin, and dihydroartemisinin. On the basis of establishing a male mouse animal model of new sporidiosis, after gavage administration, the reproductive organ index of male mice was measured at 7d, 14d, 21d, 35d, and 42d, respectively. HE staining and transmission electron microscopy were used to observe the pathological changes of testicular and epididymal tissues. The improved Pap staining method was used to analyze sperm quality, flow cytometry was used to detect apoptosis of spermatogenic cells, MDA and ACP activities were measured, and ELISA was used to detect immunoglobulin IgG1, IgG2a, IgE, and cytokine IFN-γ, IL-4, IL-6, TNF-α. The qPCR method was used to detect the expression of apoptotic genes Bax, Bcl-2, Caspase-3, P53, as well as sperm related genes C-kit, Plzf, Sycp3, Stra8, Dnajb13, Mrto4, and Ipo11, as well as the levels of NO and AsAb. The results showed that IOPs and DHA exhibited significant anti-neosporal activity. Compared to infected mice, IOPs-treated mice showed significantly increased sperm density ( < 0.05) and sperm motility ( < 0.05), while DHA-treated mice exhibited a remarkably reduced sperm deformity rate ( < 0.05). Compared with the model group, the sperm motility in the ART-treated mice was significantl upregulated ( < 0.01). Histopathological analysis revealed that all three treatments ameliorated testicular and epididymal tissue damage, reduced mitochondrial vacuolization, and improved organ indices. Biochemical assays showed a reduced level of malondialdehyde and a high level of acid phosphatase activity in the testicular tissue of treated mice. Immunological assays confirmed decreased levels of immunoglobulin G1 (IgG1), IgG2a, interleukin (IL)-4, IL-6, tumor necrosis-α factor nitric oxide, and anti-sperm antibodies in the treatment groups. Gene expression analysis indicated that IOPs significantly downregulated the expression of caspase-3, p53, and Dnajb13 and upregulated expression of SYCP3 and Stra8. There was no significant difference in the ART group ( < 0.05). DHA markedly reduced Dnajb13 expression but enhanced SYCP3, Stra8, and Ipo11 expression. These findings suggest that ART has no obvious therapeutic effect on male mice infected with Neospora caninum, IOPs and DHA can effectively mitigate N. caninum induced Spermatogenesis block, spermatozoon maturation impairment, and spermatozoon structural defects in male mice antioxidant, immunomodulatory, and antiapoptotic effects and could be considered promising candidates for anti-neosporal therapy. - Source: PubMed
Publication date: 2026/02/20
Zhao JianhaoZhao FanglinLiang YangWang ZhenyuMin PengfeiJin YixuanLi LuMa ZhenWang YangZhang XinHan SiyuanJia Lijun - Bladder cancer (BC) is the predominant malignant tumor in the urinary system globally, with its intricate molecular features greatly influencing patient outcomes and treatment response.Identifying novel biomarkers and therapeutic targets is essential for improving patient management. - Source: PubMed
Publication date: 2025/12/09
Xiao LeiWang YuanXiong YujieZhuang ZhijieHu CongXiao Zhiliang - Mesenchymal stem cell (MSC)-based therapies exhibit beneficial effects on various forms of tissue damage, including ionizing radiation-induced lesions. However, whether ionizing radiation affects the functions of human umbilical cord mesenchymal stem cells (hucMSCs) remains unclear. This study aimed to investigate the effect and possible mechanisms of ionizing radiation on the proliferation and differentiation of hucMSCs. - Source: PubMed
Publication date: 2025/04/28
Han DongmeiDing LiZheng XiaoliLi ShengYan HongminLiu JingWang Hengxiang - Colorectal cancer refers to malignant tumors occurring in the walls of the colon or rectum. The roles of WD Repeat Domain 12 (WDR12) and mitochondrial ribosome-associated tumor suppressor 4 (MRTO4) genes in colorectal cancer remain unclear. The colorectal cancer dataset GSE113513 configuration file was downloaded from the gene expression omnibus database generated from GPL15207. Differentially expressed genes screening, functional enrichment analysis, gene set enrichment analysis, Weighted Gene Co-expression Network Analysis, construction and analysis of protein-protein interaction networks, survival analysis, and gene expression heatmap plotting were conducted. Comparative toxicogenomics database analysis was performed to find diseases most relevant to core genes. TargetScan was used to screen miRNAs regulating core genes. A total of 3106 differentially expressed genes were identified. According to gene ontology analysis, they mainly enriched in organic acid metabolic processes, condensed chromosome kinetochore, oxidoreductase activity, and cell cycle. In Kyoto encyclopedia of genes and genomes analysis, they primarily concentrated in the cell cycle, TGF-β signaling pathway, Jak-STAT signaling pathway, PI3K-Akt signaling pathway, Ras signaling pathway, TNF signaling pathway, p53 signaling pathway, NF-kB signaling pathway, and WNT signaling pathway. Weighted Gene Co-expression Network Analysis with a soft thresholding power set to 12 generated 29 modules. The protein-protein interaction network identified 6 core genes (DDX27, NAT10, WDR12, DKC1, MRTO4, and NOP56). Survival analysis showed core genes (POSTN, MYH11, LUM, COL6A3, and COL4A1) as risk factors. Gene expression heatmap revealed high expression of core genes (WDR12 and MRTO4) in colorectal samples. Comparative toxicogenomics database analysis linked core genes (WDR12 and MRTO4) with local tumor infiltration, bowel obstruction, abdominal pain, and colorectal neoplasms. WDR12 and MRTO4 genes are highly expressed in colorectal cancer, potentially influencing its progression. - Source: PubMed
Luo Huanping - Long noncoding RNA (lncRNA) plays a critical role in cancer cell proliferation, invasion, metastasis, and chemoresistance. The current study introduces novel lncRNAs in colorectal cancer (CRC) through bioinformatics analysis. GSE134834 CRC-related microarray of Gene Expression Omnibus (GEO) was analyzed to identify differentially expressed genes (DEGs) in CRC samples against normal samples. Analysis revealed 6763 DEGs ( < 0.05 and |log fold change (FC)| ≥ 0.5) that include differentially expressed mRNA (DEmRNA) and differentially expressed long noncoding RNA (DElncRNA). Novel lncRNAs were identified, and to better understand the biological function of the identified lncRNAs, gene modules were constructed using weighted gene coexpression network analysis (WGCNA), and finally, two modules for lncRNAs were obtained. The coexpression modules with these lncRNAs were subjected to enrichment analysis in FunRich software to predict their functions through their coexpressed genes. Gene ontology results of modules related to novel lncRNA revealed they significantly enriched the cellular pathways regulation in cancer. The protein-protein interaction (PPI) network of novel lncRNAs-related modules was constructed using Search Tool for the Retrieval of Interacting Genes (STRING) and visualized using the Cytoscape software. Hub genes were screened from the PPI network by the CytoHubba plug-in of Cytoscape. The hub genes were , , , , , , , , , and for the lightpink4 module and , (ribosomal protein S23), (ribosomal L1 domain containing 1), , , , , (nucleolar protein 6), , and (ribosomal RNA processing 12 homolog) for the pink module. The expression levels of the top DEmRNA and module hub genes in CRC were validated using the Gene Expression Profiling Interactive Analysis (GEPIA) database. Generally, our findings offer crucial insight into the hub genes and novel lncRNAs in the development of CRC by bioinformatics analysis, information that may prove useful in the identification of new biomarkers and treatment targets in CRC; however, more experimental investigation is required to validate the findings of the present study. - Source: PubMed
Publication date: 2025/01/29
Heidari RaziehAssadollahi VahidehMarashi Seyedeh NegarElahian FatemehMirzaei Seyed Abbas