Ask about this productRelated genes to: LRP2BP Blocking Peptide
- Gene:
- LRP2BP NIH gene
- Name:
- LRP2 binding protein
- Previous symbol:
- -
- Synonyms:
- DKFZp761O0113
- Chromosome:
- 4q35.1
- Locus Type:
- gene with protein product
- Date approved:
- 2005-02-23
- Date modifiied:
- 2014-11-18
Related products to: LRP2BP Blocking Peptide
Related articles to: LRP2BP Blocking Peptide
- Diabetic nephropathy (DN) is a major complication of diabetes and a leading cause of renal failure. While valsartan has been shown to alleviate DN clinically, its antifibrotic mechanisms require further investigation. This study used a transcriptomics-driven approach, integrating in vitro, Machine Learning, molecular docking, dynamics simulations and RT-qCPR to identify key antifibrotic targets. In vitro experiments demonstrated that valsartan combats fibrosis by reversing the mRNA expression levels of fibrosis markers. PCA, t-SNE and UMAP analyses suggest the effectiveness of valsartan in modifying gene expression patterns related to fibrosis. Differential expression analysis identified key fibrosis-related genes, while WGCNA highlighted DN-associated genes in human kidney samples, with 33 potential antifibrotic targets emerging from their intersection. To enhance the accuracy of key targets selection, multiple Machine Learning algorithms-LASSO, SVM-RFE, and XGBoost-were employed, refining the potential antifibrotic targets. Molecular docking and dynamics simulations confirmed strong interactions between valsartan and targets, with RT-qPCR validating their expression reversal. GSEA indicated involvement in RAS, AGE-RAGE, TGF-beta, and PI3K-Akt pathways, affecting oxidative phosphorylation and mitochondrial regulation. These findings provide insight into therapeutic mechanisms of valsartan and demonstrate the potential of transcriptomics-driven approaches in developing targeted DN treatments. - Source: PubMed
Publication date: 2025/01/13
Wang ZewenYuan AnleiLiu ChaoqunLiu YanxiaQiao LianshengXu ZhenzhenBi ShijieTian JiayeYu BinLin ZhaozhouDu JingZhang Yanling - To investigate the role of pyroptosis in diabetic nephropathy (DN) and identify potential biomarkers for diagnosis. - Source: PubMed
Publication date: 2024/07/19
Wang QiuliZhou YanZheng NanJiang FengJuan Chenxia - Wagyu is recognized for producing marbled beef with high nutritional value and flavor. Reportedly, Wagyu has been widely used to improve the meat quality of local breeds around the world. However, studies on the genetic mechanism of meat quality in Wagyu at the whole-genome level are rarely reported. Here, whole-genome sequencing data of 11 Wagyu and 115 other individuals were used to explore the genomic variations and genes under selection pressure in Wagyu. A total of 31 349 non-synonymous variants and 53 102 synonymous variants were identified in Wagyu. The population structure analysis showed that Wagyu had the closest genetic relationship with Mishima-Ushi cattle and was apparently separated from other cattle breeds. Then, composite likelihood ratio (CLR), integrated haplotype score, fixation index and cross-population composite likelihood ratio (XP-CLR) tests were performed to identify the candidate genes under positive selection in Wagyu. In total, 770 regions containing 312 genes were identified by at least three methods. Among them, 97 regions containing 27 genes were detected by all four methods. We specifically illustrate a list of interesting genes, including LRP2BP, GAA, CACNG6, CXADR, GPCPD1, KLF2, KLF13, SOX5, MYBPC1, SLC25A10, ATP8A1 and MYH15, which are associated with lipid metabolism, fat deposition, muscle development, bone development, feed intake and growth traits in Wagyu. This is the first study to explore the genomic variations and selection signatures of Wagyu at the whole-genome level. These results will provide significant help to beef cattle improvement and breeding. - Source: PubMed
Publication date: 2023/10/04
Shi LuluHu MingyueLai WeiningYi WenfengLiu ZhengxiSun HaoLi FengYan Shouqing - Alternative polyadenylation (APA) generates different 3'-untranslated regions (3'UTRs) to regulate gene expression and localization, and affects a variety of biological processes. Here, we characterized the 3'UTR dynamics during the oocyte-to-zygote transition by analysing our previously reported porcine single-cell RNA-seq (scRNA-seq) datasets (in vitro matured metaphase II (MII) oocytes, in vitro fertilized zygotes (IVF1) and parthenogenetically activated 1-cell embryos (PA1)). After IVF1 versus MII comparison, dynamic analyses of APA from RNA-seq (DaPars) method identified 139 mRNAs with significantly different 3'UTRs (p . ≤ .05), mainly enriched in cell cycle, regulation of cyclin-dependent protein kinase activity, histone modification, mRNA surveillance, and regulation of actin cytoskeleton. For PA1 versus MII comparison, 105 mRNAs with significantly different 3'UTRs (p . ≤ .05) were identified to be mainly enriched in intracellular transport, mitotic spindle organization, cell cycle, pyruvate metabolism and glycolysis/gluconeogenesis. Furthermore, there were 7 mRNAs with more significant 3'UTR differences (|△PDUI| ≥ 0.45 and |log [PDUI ratio]| ≥ 0.59) respectively in IVF1 versus MII (Lrp2bp, Mtfr2, Nhlrc2, Psip1, Smu1, Ssr1 and Wtap) and PA1 versus MII (Asf1b, Dimt1, Nap1l1, Ncoa4, Nudt21, Pnn and Rpl15) comparisons. Integrative genomics viewer analysis further identified that 3'UTRs of Psip1, Smu1, Ssr1 and Wtap had more than 140 nt average length changes, whereas those of Dimt1, Nap1l1 and Rpl15 were shortened with more than 460 nt. Regulatory elements (PAS, CPE, microRNA binding sites and m A sites) in 3'UTRs of different lengths were predicted. Our findings provide useful information to further investigate the molecular mechanism of 3'UTR in regulating the oocyte-to-zygote transition of pig embryos. - Source: PubMed
Publication date: 2023/02/17
Zhao HanWu Zi-WeiZhang RongWang YiDu Zhi-QiangYang Cai-Xia - Genetic factors play a key role in the pathogenesis of autoimmune diseases, whereas the disease-causing variants remain largely unknown. Herein, we performed an exome-wide association study of systemic sclerosis in a Han Chinese population. In the discovery stage, 527 patients with systemic sclerosis and 5,024 controls were recruited and genotyped. In the validation study, an independent sample set of 479 patients and 1,096 controls were examined. In total, we found that four independent signals reached genome-wide significance. Among them, rs7574865 (P = 3.87 × 10) located within signal transducer and activator of transcription 4 gene was identified previously using samples of European ancestry. Additionally, another signal including three SNPs in linkage disequilibrium might be unreported susceptibility loci located in the epidermis differentiation complex region. Furthermore, two SNPs located within exon 3 of IGHM (rs45471499, P = 1.15 × 10) and upstream of LRP2BP (rs4317244, P = 4.17 × 10) were found. Moreover, rs4317244 was identified as an expression quantitative trait locus for LRP2BP that regulates tight junctions, cell cycle, and apoptosis in endothelial cell lines. Collectively, our results revealed three signals associated with systemic sclerosis in Han Chinese and suggested the importance of LRP2BP in systemic sclerosis pathogenesis. Given the limited sample size and discrepancies between previous results and our study, further studies in multiethnic populations are required for verification. - Source: PubMed
Publication date: 2020/10/15
Pu WeilinWu WenyuLiu QingmeiMa YanyunTu WenzhenZuo XianboGuo GangJiang ShuaiZhao YinhuanZuo XiaoxiaWang QingwenYang LiXiao RongChu HaiyanWang LeiSun LiangdanCui JiminYu LingLi HuiyunLi YishaShi YaqianZhang JiaqianZhang HaishunLiang MinruiChen DongdongDing YueChen XiangxiangChen YuanyuanZhang RuiZhao HanLi YuanQi QingBai PengZhao LiangReveille John DMayes Maureen DJin LiLee Eun BongZhang XuejunXu JinhuaZhang ZhengZhou XiaodongZou HejianWang Jiucun