Ask about this productRelated genes to: ADSL Blocking Peptide
- Gene:
- ADSL NIH gene
- Name:
- adenylosuccinate lyase
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 22q13.1
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2014-11-18
Related products to: ADSL Blocking Peptide
Related articles to: ADSL Blocking Peptide
- Toxoplasma gondii is an infectious disease that infects nearly one third of the world's population and endangers the health of immunocompromised people, pregnant women, and livestock. There are no existing drugs able to treat the infection and prevent tissue cysts from developing. Therefore, the creation of a safe vaccine should be considered as a matter of great importance. While many of the vaccines have not proven successful in the past, recently created live-attenuated vaccines (LAVs) using CRISPR-Cas9 gene editing technology were able to outperform the previous ones. The current review aims to highlight the recent progress in genetically engineered LAVs against T. gondii. In particular, the knockout strains affecting metabolic genes (ompdc, uprt, and adsl), virulence genes (rop18), and host-parasite interactions (gra5, gra72, had2a, and cdpk3) will be mentioned. LAVs described above demonstrate high attenuating effects and ability to protect mice by inducing immunity on the basis of specific IgG (IgG2a), IFN-γ, IL-12, and CD4, CD8 T cells. Furthermore, vaccination provides protection from a lethal infection caused by types I, II, and Chinese 1 strains of T. gondii as well as preventing development of tissue cysts in chronic infection. RHΔompdcΔuprt is an example of an LAV that can be used to reduce oocyst shedding in cats and promote the One Health concept. In general terms, genetically engineered LAVs can effectively deal with toxoplasmosis infection with better attenuation immunogenicity balance than other vaccines. Nonetheless, several problems need consideration. - Source: PubMed
Publication date: 2026/06/07
Sang XiaoyuZhang HuaZhang YutongJin YantingSun FangqiFeng YingAl-Olayan EbtesamYang NaEl-Ashram Saeed - Glioblastoma (GBM) remains a lethal brain tumor with limited prognostic tools. Metabolic reprogramming, particularly in understudied pathways like propionate metabolism, may offer new biomarkers. Here, we identified a novel prognostic signature based on seven propionate metabolism-related genes (SLC9A1, ELANE, ACADS, SOAT2, MYD88, ADSL, and BMP2) from the TCGA-GBM cohort. A risk scoring model was constructed via LASSO Cox regression effectively stratified patients into high- and low-risk groups with significant survival differences, which was also validated in independent GEO datasets. Multiomics analysis revealed that the high-risk group was associated with an immunosuppressive microenvironment, characterized by increased immune checkpoint expression and distinct immune cell infiltration. Mutational profiling showed a strong association with key driver alterations, including enrichment of RB1 mutations in high-risk and IDH1 mutations in low-risk patients. Single-cell RNA-seq (scRNA-seq) analysis confirmed the specific enrichment of signature genes within malignant cells, and coexpression network analysis (hdWGCNA) further linked the high-risk phenotype to transcriptional modules. In conclusion, we established and validated a robust metabolic gene signature that not only predicts prognosis but also delineates a high-risk GBM subtype defined by integrated metabolic, immunogenomic, and transcriptional features, providing new insights into the determinants of GBM aggressiveness. - Source: PubMed
Publication date: 2026/05/16
Li MengtongLiu JiayiLiu ZichenLiu XiaLun Peng - Leishmania donovani ADSL is a tetrameric protein involved in the purine metabolism where the active site is formed from residues belonging to three different subunits. In this study, the amino acid residues- E334, N335 and R370 of LdADSL were studied for their contribution to the enzyme catalysis and inter-subunit binding. Mutating these residues resulted in reduced activity, stability and affinity towards the substrate, SAMP/AMP as shown by enzyme kinetics, fluorescence spectroscopy and thermal stability studies. MD simulation studies also supported this. Structural analysis points to disrupted interactions with the catalytic base, H196, reduced hydrogen bonds and electrostatic interactions between the substrate and the enzyme, C3-loop conformational changes and altered conformation of active site residues as reasons for reduced activity and stability. Incubation of pairs of the mutant enzymes restored partly the functional active site by subunit complementation. Our study highlighted the critical role of inter-subunit residues in maintaining both structural stability and proper active-site architecture in enzymes whose functional catalytic site is formed at interfaces of different subunits. This knowledge can be used to design more specific anti-leishmanials by targeting the inter-subunit interface of LdADSL. - Source: PubMed
Publication date: 2026/05/15
Jigneshkumar A MochiJaykumar JaniAnju Pappachan - As a precursor to creatine, guanidinoacetic acid (GAA) is widely recognized to enhance growth performance and flesh quality of animals, but the underlying molecular mechanism remains unclear. This study evaluated the effects of dietary GAA supplementation on growth performance, textural properties, and flavor constituents in gibel carp CAS V (, CAS V). A total of 300 healthy gibel carp (5.01 ± 0.13 g) at 42 d of age were assigned randomly to 12 tanks (3 replicates per group, 25 fish per replicate). Fish were fed with a basal diet supplemented with graded levels of GAA (0.00, 0.03%, 0.06%, and 0.12%) for 10 weeks. Results demonstrated that GAA supplementation significantly enhanced the specific growth rate (SGR) and crude protein content in gibel carp ( < 0.05). It also enhanced muscle physicochemical attributes by increasing water holding capacity and hardness, along with boosting glycogen content and free glutamate level ( < 0.05). Mechanistically, GAA promoted myofiber development by upregulating protein and gene expression of myogenic regulatory factors (MRFs), leading to increase myofiber density and a higher frequency of myofibers with diameters between 20 and 40 μm ( < 0.05). Furthermore, GAA facilitated collagen synthesis to improve muscle hardness by activating the transforming growth factor-beta 1 (TGF-β1)/mothers against decapentaplegic homolog (SMADs) signaling pathway, upregulating transcript levels of , , , and ( < 0.05). Additionally, GAA increased inosine monophosphate (IMP) content in muscle ( = 0.015), which was associated with enhanced expression of AMPD1 protein and upregulation of the , , and genes ( < 0.05). In conclusion, dietary GAA supplementation enhanced flesh quality of gibel carp via improving growth performance, nutrient deposition, texture characteristics, and flavor components. - Source: PubMed
Publication date: 2026/04/03
Wang YuZhang YanWu LiyunLi ChaoyueChen QiaozhenHan DongLiu HaokunZhang ZhiminXie ShouqiJin Junyan - Cochlear implantation is a common treatment for adults with single-sided deafness (SSD), but patient-reported benefits vary. The relationships among tinnitus burden, perceived hearing ability, psychological distress, disease-specific health-related quality of life, and whether early postoperative outcomes predict later results are not well understood. - Source: PubMed
Publication date: 2026/04/15
Schrader Jasper Karl FriedrichGröschel MoritzSzczepek Agnieszka JOlze Heidi