TADA2L Blocking Peptide
- Known as:
- TADA2L Blocking Peptide
- Catalog number:
- 33r-8153
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Fitzgerald industries international
- Gene target:
- TADA2L Blocking Peptide
Related genes to: TADA2L Blocking Peptide
- Gene:
- TADA2A NIH gene
- Name:
- transcriptional adaptor 2A
- Previous symbol:
- TADA2L
- Synonyms:
- ADA2, hADA2, ADA2A
- Chromosome:
- 17q12
- Locus Type:
- gene with protein product
- Date approved:
- 1996-12-17
- Date modifiied:
- 2016-10-05
Related products to: TADA2L Blocking Peptide
Related articles to: TADA2L Blocking Peptide
- Marker-assisted selection has increasingly relied on single-nucleotide polymorphisms (SNPs) as robust genetic markers, particularly in livestock breeding programs. In pig farming, embryonic mortality significantly affects litter size, and SNPs in reference genes have been implicated as potential causal factors. We developed and optimized a tetra-primer amplification refractory mutation system (T-ARMS) PCR assay for rapid, cost-effective detection of SNPs in 3 candidate genes-, , -that are associated with embryonic lethality and reproductive performance. Primer sets were designed based on known mutation sites and validated using synthetic gene constructs and porcine genomic DNA from pigs of Duroc and Landrace breeds. Optimization of annealing temperatures and primer concentration ratios yielded distinct and reproducible allele-specific amplicon patterns that were corroborated by PCR-RFLP and Sanger sequencing. Our T-ARMS PCR protocol, which requires minimal equipment and reduces processing time to <3 h, had high specificity and efficiency in differentiating wild-type, heterozygous, and homozygous mutant genotypes in 20 Duroc and 20 Landrace pigs. Our Tetra-ARMS PCR assay is a robust and economically viable tool for SNP genotyping in pig breeding programs, potentially contributing to the reduction of embryonic lethality and the improvement of overall reproductive outcomes. - Source: PubMed
Publication date: 2026/01/22
Minh Nhut PhamNguyen Nghiep MaiBui Anh Phu Nam - Increasing the number of live-born piglets per litter is one of the major objectives of pig breeding programs. Single nucleotide polymorphisms (SNPs) in polynucleotide kinase-phosphatase (), RNA polymerase I subunit B (), ribosome biogenesis (), and transcriptional adaptors ADA2 ( genes are recessive lethal alleles that are associated with embryonic lethality, which affects the reproductive performance and animal welfare of pig populations). No assay is available to screen for the recessive haplotypes of these 4 SNPs in pig populations. We successfully developed and verified a rapid, simple, and cost-effective PCR-restriction-fragment length polymorphism (PCR-RFLP) assay for genotyping SNPs in , , genes in Landrace and genes in Duroc pigs in Vietnam. Our PCR-RFLP approach could potentially be applied in screening programs on a larger scale to improve the reproductive performance of commercial pig populations. - Source: PubMed
Publication date: 2025/08/07
Bui Anh Phu NamNguyen Nghiep MaiLe Quang - Dysregulation of circRNAs has been found to engage in the progression of many hematologic malignancies including acute myeloid leukemia (AML). In this study, we identified significantly downregulated circTADA2A, derived from linear transcriptional adaptor 2A (TADA2A) gene, in AML associated circRNAs microarrays using GEO2R tool. We aimed to elucidate the roles of circTADA2A in AML and the mechanisms involved. Quantitative reverse transcription PCR was used for verification of circTADA2A levels in AML specimens, and its diagnostic value and clinical significance were assessed. The effects of circTADA2A on proliferation and ferroptosis on THP-1 and HL-60 cells were carried out using cell counting kit-8, 5'-ethynyl-2'-deoxyuridine, Fe, lipid reactive oxygen species (ROS) and malondialdehyde (MDA) assays. Luciferase reporter, fluorescence in situ hybridization, RNA immunoprecipitation, and RNA pull-down assays were implemented to investigate the potential miRNAs that mediated circTADA2A functioning. We confirmed that circTADA2A levels were lowly expressed in plasma and bone marrow of AML patients, and associated with bone marrow blasts and cytogenetic risk. Plasma circTADA2A had a high sensitivity and specificity with an area under the curve value of 0.793 in differentiating AML patients from healthy individuals. THP-1 and HL-60 cells stably overexpressing circTADA2A exhibited reduced cell proliferation, and sensitized cell to ferroptosis by a ferroptosis inducer RSL3. Moreover, circTADA2A could counteracted Ferrostatin-1-induced inhibition of ferroptosis. Mechanistically, circTADA2A act as a sponge for miR-638, and upregulation of miR-638 expression could restore cellular phenotypes induced by circTADA2A. Our findings demonstrated that circTADA2A suppresses cell proliferation and promotes ferroptosis by sponging miR-638 during AML progression. - Source: PubMed
Publication date: 2025/07/03
Yuan YuanLi JiajiaWang MengJin YanXia Ruixiang - Prostate cancer (PCa) belongs to a prevailing neoplasm globally. Circular RNAs (circRNAs) are critical regulators in various tumors, but the role of circRNAs in PCa is obscure. In this research, a circRNA derived from the TADA2A gene (hsa_circ_0006220) was high-expressed in PCa tissues along with cell lines. Elevated Circ-0006220 expression was also related to PCa poor prognosis. Besides, circ-0006220 accelerated PCa cells malignant behaviors in vitro; it also promoted PCa tumor growth together with metastasis in vivo. Moreover, circ-0006220 competed with the Cell Division Cycle Associated 7 (CDCA7) for binding to miR-520f-3p. Circ-0006220 sponged miR-520f-3p to regulate CDCA7 expression, thereby promoting PCa cell proliferation, migration, invasion, along with metastasis. All above data suggested that circ-0006220 may be a worthy target for PCa therapeutics. - Source: PubMed
Publication date: 2024/07/28
Wan ZijinLiu Gang - Eggshell translucency is a widespread issue in the field of egg quality. Previous research has established that the heritability of eggshell translucency is relatively low or moderate. Scientists have also successfully identified SNP loci related to eggshell translucency on different chromosomes by using gene chips and single-variant GWAS. However, the specific impact of single or multiple genes on the trait of eggshell translucency remains unknown. In an effort to investigate this, we examined 170 SNPs associated with eggshell translucency obtained by our research group. We selected 966 half-sibling laying hens from 2 generations in 3 pure lines: Dwarf Layer-White, Rhode Island Red-White Strain, and Rhode Island Red. Eggs were collected from each hen over a period of 5 consecutive days, and eggshell translucency was measured using a grading method in which the hens were divided into 2 groups: an opaque group and a translucent group. We collected blood samples from the laying hens and extracted DNA. Time of flight mass spectrometry (TOF-MS) was used for genotyping to identify SNP loci that influence the trait of eggshell translucency. The results of our analysis revealed that using TOF-MS in 3 chicken strains, we were able to eliminate loci with low gene polymorphism, genetic effect contribution less than 1%, and deviation from Hardy-Weinberg equilibrium. Ultimately, 5 SNPs (Affx-50362599, rs15050262, rs312943734, rs316121113, and rs317389181) were identified on chromosomes 1, 5, and 19. Additionally, nine candidate genes (DCN, BTG1, ZFP92, POU2F1, NUCB2, FTL, GGNBP2, ACACA, and TADA2A) were found to be associated with these SNPs. No linkage disequilibrium relationship was observed between the 2 pairs of SNP loci on chromosomes 1 and 19. Based on previous studies on the formation mechanism of eggshell translucency, we hypothesize that NUCB2, FTL, and ACACA genes may be affecting the eggshell structure through different mechanisms, such as increase the water permeability or make thin of eggshell membrane, which promote moisture or part of other egg contents and ultimately lead to the formation of eggshell translucency. These findings validate and identify five SNP loci that regulate the translucency trait, and provide molecular markers for breeding non-translucent populations. Furthermore, this study serves as a reference for further investigation of the genetic regulatory mechanisms underlying eggshell translucency. - Source: PubMed
Publication date: 2024/06/19
Liu Geng-YunChen Xiang-YuLiu Xue-LuZhou Rong-YanZhao Xiao-YuXu Li-JunNing Zhong-HuaWang De-He